Evaluation of the biological properties of soluble chitosan and chitosan microspheres

Carreño-Gómez, Begoña; Duncan, Ruth

doi:10.1016/s0378-5173(96)04847-8

Cited by 284 publications

(129 citation statements)

References 11 publications

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“…20,35 The present study found that an ultrapure chitosan, UPC, was essentially nontoxic, while the corresponding chitosan produced in our laboratory C(15;190), had a low but detectable in vitro toxicity at high doses. This suggests that the toxicity most likely is due to impurities in the chitosans.…”

Section: After 24 H the Membranes Of Endosomal Compartments Filledmentioning

confidence: 51%

Chitosan as a nonviral gene delivery system. Structure–property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo

et al. 2001

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Chitosan is a natural cationic linear polymer that has recently emerged as an alternative nonviral gene delivery system. We have established the relationships between the structure and the properties of chitosan-pDNA polyplexes in vitro. Further, we have compared polyplexes of ultrapure chitosan (UPC) of preferred molecular structure with those of optimised polyethylenimine (PEI) polyplexes in vitro and after intratracheal administration to mice in vivo. Chitosans in which over two out of three monomer units carried a primary amino group formed stable colloidal polyplexes with pDNA. Optimized UPC and PEI polyplexes protected the pDNA from serum degradation to approximately the same degree, and they gave a comparable maximal transgene expression in 293 cells. In contrast to PEI, UPC was non toxic at escalating doses. After intratracheal administration,

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Section: After 24 H the Membranes Of Endosomal Compartments Filledmentioning

confidence: 51%

Chitosan as a nonviral gene delivery system. Structure–property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo

et al. 2001

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“…Cytotoxicity against Caco-2 and U937 cells was similar to concentrations found for 43 kDa pDMAEMA against human brain microvascular endothelial cells 66 . Cytotoxicity against the human cell lines was also similar to the antimicrobial polymer, chitosan, against B16F10 murine melanoma cell line and the cationic reference polymer, poly(L-lysine) 69 . Against Caco-2 cells none of the analogues produced significantly different cytotoxicity concentrations than the unconjugated polymer, except for the hostasol-tagged polymer after 24 hours.…”

Section: Discussionmentioning

confidence: 69%

Antibacterial Effects of Poly(2-(dimethylamino ethyl)methacrylate) against Selected Gram-Positive and Gram-Negative Bacteria

et al. 2009

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Antimicrobial coatings can reduce the occurrence of medical device-related bacterial infections. Poly(2-(dimethylamino ethyl)methacrylate) (pDMAEMA) is one such polymer that is being researched in this regard. The aims of this study were to (1) elucidate pDMAEMA’s antimicrobial activity against a range of Gram-positive and Gram-negative bacteria and (2) to investigate its antimicrobial mode of action. The methods used include determination of minimum inhibitory concentration (MIC) values against various bacteria and the effect of pH and temperature on antimicrobial activity. The ability of pDMAEMA to permeabilise bacterial membranes was determined using the dyes 1-N-phenyl-naphthylamine and calcein-AM. Flow cytometry was used to investigate pDMAEMA’s capacity to be internalized by bacteria and to determine effects on bacterial cell cycling. pDMAEMA was bacteriostatic against Gram-negative bacteria with MIC values between 0.1−1 mg/mL. MIC values against Gram-positive bacteria were variable. pDMAEMA was active against Gram-positive bacteria around its pK a and at lower pH values, while it was active against Gram-negative bacteria around its pK a and at higher pH values. pDMAEMA inhibited bacterial growth by binding to the outside of the bacteria, permeabilizing the outer membrane and disrupting the cytoplasmic membrane. By incorporating pDMAEMA with erythromycin, it was found that the efficacy of the latter was increased against Gram-negative bacteria. Together, the results illustrate that pDMAEMA acts in a similar fashion to other cationic biocides.

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“…In vivo chronic toxicity studies reported by Carreño-Gómez and Duncan (Carreño-Gómez & Duncan, 1997) have shown that intravenously administered chitosan (4.5 mg/kg/day for 11 days) to rabbits did not lead to any abnormal changes, while doses of 50 mg/kg/day induced death after the third day of exposure to chitosan. However, oral administration of chitosan at doses of 700-800 mg/kg/day to rabbits or hens during 34 weeks produced little toxic effects (e.g., loss of appetite in hens).…”

Section: In Vivo Toxicity Of Chitosanmentioning

confidence: 92%

“…Scarce data are available for different salts of chitosan such as hydroglutamate, glycol, hydrochloride and hydrolactate. All of these compounds were shown to have some cytotoxicity against B16F10 (murine melanoma) cells, with the highest toxicity being exerted by chitosan hydrochloride salt (IC 50 = 0.21 ± 0.04 mg/mL, MW > 100 kDa) (Carreño-Gómez & Duncan, 1997). Ribeiro et al, 2009 studied the in vitro cytotoxicity of chitosan hydrogels when tested with dermal fibroblasts obtained from rat skin.…”

Section: In Vitro Toxicity Of Chitosanmentioning

confidence: 99%

Chitosan as a starting material for wound healing applications

Patrulea

Ostafe

Borchard

et al. 2015

European Journal of Pharmaceutics and Biopharmaceutics

491

215

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Abstract:Chitosan and its derivatives have attracted great attention due to their properties beneficial for application to wound healing. The main focus of the present review is to summarize studies involving chitosan and its derivatives, especially N,N,N-trimethylchitosan (TMC), N,O-carboxymethyl-chitosan (CMC) and O-carboxymethyl-N,N,Ntrimethyl-chitosan (CMTMC), used to accelerate wound healing. Moreover, formulation strategies for chitosan and its derivatives, as well as their in vitro, in vivo and clinical applications in wound healing are described.

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Evaluation of the biological properties of soluble chitosan and chitosan microspheres

Cited by 284 publications

References 11 publications

Chitosan as a nonviral gene delivery system. Structure–property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo

Chitosan as a nonviral gene delivery system. Structure–property relationships and characteristics compared with polyethylenimine in vitro and after lung administration in vivo

Antibacterial Effects of Poly(2-(dimethylamino ethyl)methacrylate) against Selected Gram-Positive and Gram-Negative Bacteria

Chitosan as a starting material for wound healing applications

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