Evaluation of the combination of the NucliSENS easyMAG® and the EasyQ® applications for the detection of Mycoplasma pneumoniae and Chlamydia pneumoniae in respiratory tract specimens

Abstract

Mycoplasma pneumoniae and Chlamydia pneumoniae are respiratory tract pathogens frequently involved in community-acquired pneumonia, but are fastidious microorganisms. Their direct detection mainly requires molecular amplification techniques. A nucleic acid extraction system, NucliSENS easyMAG, and a real-time nucleic acid sequence-based amplification (NASBA) technique, NucliSENS EasyQ, were recently developed by bioMérieux to detect both bacteria. The aim of our study was to compare the easyMAG/EasyQ combination with our in-house combination, MagNA Pure extraction (Roche) and real-time polymerase chain reaction (PCR), to detect both bacteria in respiratory tract specimens. The analytical specificities of both combinations were similar. A higher analytical sensitivity was found for C. pneumoniae using the easyMAG/EasyQ combination, since the easyMAG/EasyQ system detected nucleic acid extracts 10(6) times more diluted than the in-house combination. Both combinations were equivalent when detecting M. pneumoniae in positive respiratory tract samples. Finally, the easyMAG/EasyQ combination is a potential useful tool for the detection of both bacteria regarding sensitivity, specificity, monitoring, and standardization of the procedure.

Characterization of Pontibacter altruii , sp. nov., isolated from a human blood culture

“…Total DNA was extracted from the isolate using a generic lysis and silica adsorption-based separation protocol (NucliSENS ® EasyMAG ® , bioMérieux; [4], [5]). A portion of the 16S ribosomal RNA gene was amplified with primers 27-F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492-R (5′-ACGGCTACCTTGTTACGACTT-3′) [6] using Roche Life Science Expand High Fidelity plus reagents according to the manufacturer's recommendations (Roche, Basel, Switzerland).…”

Advances in nanomaterials and their applications in point of care (POC) devices for the diagnosis of infectious diseases

“…AuNPs have also been integrated into the design of disposable biosensors. They were used together with screen-printed electrodes for the diagnosis of respiratory pathogens such as Mycoplasma pneumonia, Streptococcus pneumonia, and Chlamydophila pneumonia (Bessede et al, 2010).…”

Pooled analysis of nuclear acid sequence‐based amplification for rapid diagnosis of Mycoplasma pneumoniae infection

“…One article with incomplete original data and one article with data published repeatedly were further excluded after reading the full text. Finally, six articles [30][31][32][33][34][35] meeting the inclusion criteria were included in this meta-analysis.…”