“…Analysis of variance (ANOVA and MANOVA) has been used to investigate the influence of location on forms of metals in roadside soil (Nowak, 1995). Multiple regression analysis has proved valuable in processing sequential extraction data to obtain information on plant availability of trace metals in soils (Qian et al, 1996; 280 Speciation in compartments of the environment Li et al, 1995b (modified Tessier) SRM 2711 d Fe, K, Mn, Ni, P, Pb, Sr, Ti, V, Zn 5-stage MAG-1 a , LKSD-4 b , TILL-2 b , As, Be, Ca, Cd, Ce, Hall et al, 1996 SO-1 Zhang et al, 1998) whilst, in the aquatic environment, chemometric methods have provided insight into sources of heavy metal contamination (Borovec, 1996;Tsai et al, 1998). Chemometrics can also be used to overcome some of the intrinsic deficiencies of sequential extraction, such as non-specificity.…”