Evaluation of the cytotoxic and spasmolytic activities of Solanum asperum Rich. (Solanaceae).

Abstract

Studies were conducted to determine the haemolytic activity of the methanol extract obtained from roots of Solanum asperum (SAr-MeOH) on rat erythrocytes and its spasmolytic activity on guineapig trachea and ileum. Blood samples were collected from rats through cardiac puncture to obtain erythrocyte suspensions for the evaluation of cytotoxic potential of SAr-MeOH by haemolysis. The relaxation of the guineapig trachea was determined by calculating the percentage of reverse carbachol-induced initial contraction after the addition of SAr-MeOH. Inhibition of response to submaximal carbachol in the guineapig ileum was assessed by comparing the responses before (control) and after addition of SAr-MeOH. Results showed that the SAr-MeOH extract showed low damage to the rat erythrocytes and the concentration tested on guineapig ileum had no toxic effect. This suggested that SAr-MeOH had low or no toxicity when tested in vivo. It was also shown that the SAr-MeOH extract had secondary metabolites with low toxicity and a potential spasmolytic activity on guineapig ileum. On the other hand, the extract did not show any effect on guineapig trachea.

Toxicological, Antidiarrheal and Spasmolytic Activities of Solanum paniculatum

“…Because some Solanum species have cytotoxic effects, such as Solanum asperum Rich. and S. asterophorum, and present moderate hemolytic activity in rat erythrocytes [27,28], we initially decided to observe the cytotoxic potential and possible acute toxicity of SP-EtOH AP using in vitro and in vivo models, respectively. In the cytotoxicity assay, SP-EtOH AP (125, 250, 500, and 750 µg/ mL, n = 3) did not induce rat erythrocytes lysis (l " Fig.…”