Evaluation of the detection of melanin by the Fontana-Masson silver stain in tissue with a wide range of organisms including Cryptococcus

Bishop, Justin A.; Nelson, Ann Marie; Merz, William G.; Askin, Frederic B.; Riedel, Stefan

doi:10.1016/j.humpath.2011.07.021

Cited by 28 publications

(16 citation statements)

References 21 publications

(23 reference statements)

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“…Lungs and subcutaneous melanoma tumors obtained from mice with melanoma were fixed in 4% paraformaldehyde (Electron Microscopy Sciences) and conventionally processed for histology using Fontana-Masson 18 and Hematoxylin-Eosin 19 staining. For immunohistochemistry and immunofluorescence techniques the following antibodies were used: Ki-67 (clone SP6 e Biocare Medical), Beta-catenin (rabbit polyclonal e EMD Millipore), active caspase-3 (rabbit polyclonal e Biocare Medical), P-Selectin (goat polyclonal e Santa Cruz Biotechnology), and a biotinylated probe for Hyaluronic Acid.…”

Section: Histologymentioning

confidence: 99%

“…Probes were a kind gift of Doctor Helena Nader (Universidade Federal de São Paulo, São Paulo, Brasil), who produces in strict accordance with the previously described method. 18 Nonspecific biding was blocked by incubation with 1% bovine serum albumin (BSA) in PBS for 10 min prior to antibodies staining. Incubations were conducted overnight at 4 C. Immunohistochemistry revelations were performed with the phosphatase/fast red system (catalog number MRCT523G, H, L e Biocare Medical) or the 3,3 00 -diaminobenzidine tetrahydrochloride (DAB) Substrate Kit (BD PharmigenÔ) and further stained with hematoxylin or fast green.…”

Section: Histologymentioning

confidence: 99%

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Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

et al. 2016

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Altogether these data suggest M1 is an efficient candidate for melanoma therapy to be considered for future clinic studies as this study is the first supporting the idea that melanoma patients may benefit with the treatment. The treatment with M1 provides advantages considering the highly-diluted properties and a cost effective alternative to costly chemotherapeutic approaches with, if any, lower toxicity.

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Section: Histologymentioning

confidence: 99%

Section: Histologymentioning

confidence: 99%

Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

et al. 2016

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“…Fungal detection is almost the same as that of bacteria—they require their own special staining, media and biochemical reactions [154,155,156,157,158]. Fungal cultures are also time consuming and laborious [159].…”

Section: Dep Applications In Biomedical Sciencesmentioning

confidence: 99%

Dielectrophoresis for Biomedical Sciences Applications: A Review

Rahman

Ibrahim

Yafouz

2017

Sensors

174

155

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Dielectrophoresis (DEP) is a label-free, accurate, fast, low-cost diagnostic technique that uses the principles of polarization and the motion of bioparticles in applied electric fields. This technique has been proven to be beneficial in various fields, including environmental research, polymer research, biosensors, microfluidics, medicine and diagnostics. Biomedical science research is one of the major research areas that could potentially benefit from DEP technology for diverse applications. Nevertheless, many medical science research investigations have yet to benefit from the possibilities offered by DEP. This paper critically reviews the fundamentals, recent progress, current challenges, future directions and potential applications of research investigations in the medical sciences utilizing DEP technique. This review will also act as a guide and reference for medical researchers and scientists to explore and utilize the DEP technique in their research fields.

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“…Unlike other largely used staining, Masson-Fontana is not a routine technique and it is more focused on the specific detection of melanin in malignant melanomas as well as in some benign tumors derived from melanocytes (136). Furthermore, many years ago it was reported the atypical use of Fontana-Masson as a relatively specific stain for the diagnostic of infectious caused by certain Cryptococcus, a procedure that is also currently applied (137)(138).…”

Section: Pigments Identificationmentioning

confidence: 99%

Dyes and Stains: from molecular structure to histological application

2014

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In the present review, the chemistry of dyes as well as the interaction mechanisms between tissue and dye has been detailed, and also some of the key factors affecting the selectivity of dyes by certain cellular structures have been mentioned. Moreover, due to the relevance that histological stains have acquired in biomedical research, some of the most common stains have been described, pointing out previous and current applications in basic and applied research.

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Evaluation of the detection of melanin by the Fontana-Masson silver stain in tissue with a wide range of organisms including Cryptococcus

Cited by 28 publications

References 21 publications

Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

Dielectrophoresis for Biomedical Sciences Applications: A Review

Dyes and Stains: from molecular structure to histological application

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