Evaluation of the Direct and Enrichment Iso 10272 Methods for the Detection of Campylobacter Spp. in Chicken Meat

Benetti, Thalyta Marina; Abrahão, Wanda Moscalewski; Ferro, ID; Macedo, Renata Ernlund Freitas de; Oliveira, Tcrm

doi:10.1590/1516-635x1801023-028

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Furthermore, due to the difficulty of isolating these bacteria in addition to their nutritional and environmental requirements (3), a lot of studies indicate that false-negative results have been observed during the detection of Campylobacter spp. in food, notably in poultry meat (3,4) and human fecal specimens with rates ranging from 24% to 40% (5,6). These alarming observations indicate that the use of appropriate enrichment broths, selective agar media, and specific conditions that allow regular growth are important for the recovery of C. spp.…”

Section: Introductionmentioning

confidence: 99%

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Campylobacter spp. at different stages of the poultry slaughtering line in Algeria: Evaluation of direct and indirect modified ISO 10272:2017 detection methods and characterization of the isolates

2023

ARI

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This study aimed to evaluate the effectiveness of direct and indirect modified ISO 10272-1:2017 methods for detecting Campylobacter spp. in 10 sites of a poultry slaughterhouse and investigate the relationship between poultry intestinal carriage and carcasses, as well as surfaces contamination during different slaughter steps (scalding, defeathering, evisceration, and rinsing). Antibiotic resistance profiles of the isolates were also determined against 12 antibiotics. A total of 165 intestinal (feces and ceca) and non-intestinal (neck skins and surfaces) samples were collected from 10 different sampling sites before, during, and after the slaughtering of six flocks of broiler chickens. After the isolation and phenotypic identification of the isolates, an antibiotic susceptibility study was performed using the agar diffusion method. Thermotolerant bacteria of the genus Campylobacter (TC) were isolated with a prevalence of 47.04% (127/270), and 39.05% (82/210) of the TC isolates were detected in non-intestinal samples. Moreover, 76.19% (80/105) of these microorganisms were detected by a direct isolation method for a sensitivity of 97.56%, while only 1.90% (2/105) of the samples contained TC by an indirect isolation method for a sensitivity of 2.44%. The samples of intestinal origin were positive for TC with a rate of 75.00% (45/60). C. jejuni (76.38%; 97/127) was the most isolated bacterial species. Furthermore, 98.43% (125/127) of the TC isolates were multidrug-resistant and 69.29% (88/127) showed simultaneous resistance to ciprofloxacin and erythromycin. Direct isolation seems to be the best method for the detection of C. spp. A serious public health problem of multidrug-resistant C. spp. isolates with critical resistance profiles can be transmitted to broiler carcasses before, during, and after the evisceration step.

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Section: Introductionmentioning

confidence: 99%

“…These alarming observations indicate that the use of appropriate enrichment broths, selective agar media, and specific conditions that allow regular growth are important for the recovery of C. spp. from poultry meat (3,4) and other types of samples.…”

Section: Introductionmentioning

confidence: 99%

Campylobacter spp. at different stages of the poultry slaughtering line in Algeria: Evaluation of direct and indirect modified ISO 10272:2017 detection methods and characterization of the isolates

2023

ARI

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Current methodologies and future direction of Campylobacter isolation and detection from food matrices, clinical samples, and the agricultural environment

Harrison

Balan²,

Hiett³

et al. 2022

Journal of Microbiological Methods

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Occurrence and diversity of Campylobacter species in diarrheic children and their exposure environments in Ethiopia

Belina,

Gobena,

Kebede

et al. 2024

PLOS Glob Public Health

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Campylobacter is a major zoonotic foodborne pathogen that poses a significant public health threat, particularly among children and immunocompromised individuals. However, data on the occurrence and sources of Campylobacter infection remain scarce in Ethiopia. This study assessed the occurrence, diversity, and relationships between Campylobacter from diarrheic children and potential exposure sources using whole-genome sequencing. Through case-based tracing, animal, food, and environmental samples were collected from Harar town and Kersa district between November 2021 and January 2023. Campylobacter was identified using selective media, and DNA was extracted and sequenced with the Illumina NextSeq 550 instrument. Sequence reads were analysed using bioinformatics tools. The overall Campylobacter prevalence in the exposure sources was 5.5%, with 6.0% in urban and 5.0% in rural settings. Campylobacter detection was 1.8 times more likely in household samples (8.7%; OR = 1.8; 95% CI: 0.7–4.5) than in samples from marketplaces. The occurrence of Campylobacter in food was 4.2%, with no significant differences across the meat, milk, and other food categories. The likelihood of Campylobacter contamination in the environment was 5.8 times higher in the presence of poultry (17.7%; OR = 5.8; CI: 1.1–30.6) compared to shoats. Sequence analysis identified a low Campylobacter spp. diversity comprising only C. jejuni and C. coli, which were characterized by 8 distinct sequence types (STs). Phylogenetically, the majority of the sequenced case isolates were clustered with isolates from either caretakers, environmental exposures, or both. In conclusion, Campylobacter was detected in various exposure sources of diarrheic children, and its occurrence did not differ significantly between Kersa and Harar or among food items. The majority of isolates shared MLST profiles and clustered together, demonstrating the involvement of multiple vectors in the transmission of the pathogen. Genome-based integrated studies supported by an attribution model are recommended to determine the relative contribution of each source.

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Evaluation of the Direct and Enrichment Iso 10272 Methods for the Detection of Campylobacter Spp. in Chicken Meat

Cited by 3 publications

References 18 publications

Campylobacter spp. at different stages of the poultry slaughtering line in Algeria: Evaluation of direct and indirect modified ISO 10272:2017 detection methods and characterization of the isolates

Campylobacter spp. at different stages of the poultry slaughtering line in Algeria: Evaluation of direct and indirect modified ISO 10272:2017 detection methods and characterization of the isolates

Current methodologies and future direction of Campylobacter isolation and detection from food matrices, clinical samples, and the agricultural environment

Occurrence and diversity of Campylobacter species in diarrheic children and their exposure environments in Ethiopia

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