Evaluation of the effect of terpenoid quinones on Trichophyton mentagrophytes by solution and vapor contact

The vapor activity of oregano, perilla, tea tree, lavender, clove, and geranium oils against a Trichophyton mentagrophytes in a closed box

“…The detailed procedure for the inocula and the box vapor assay, using agar plugs containing fungal cells grown on the surface has been described previously. 5 The number of fungal cells was estimated from the colony size of agar plugs exposed to the oil vapor in a closed box followed by incubation at 27°C for 5 days in a fresh medium. The colony size showed a linear correlation with the number of conidia between 10 3 and 10 6 .…”

Antimycotic potential of a diterpenoid taxoquinone against <i>Candida</i> species isolated from <i>Metasequoia glyptostroboides</i>

“…A diterpenoid taxoquinone exhibited a wide range of anticandidal activity against the tested isolates of Candida species. Several terpenoid compounds have been shown to exert inhibitory effect against the fungal pathogens of Candida species (Inouye et al, 2006;Picman et al, 1990;Rao et al, 2010). Previous studies suggest that various terpenoid compounds of plant origin can be available for trials to control severe fungal infections caused by Candida species.…”

Vapor activity of 72 essential oils against a Trichophyton mentagrophytes

“…12 That is, 30 µl of a 1%, 5%, or undiluted essential oil or pure compound dissolved in ethyl acetate was put on a disc, which was placed at the center of double-layered agar medium in a petri dish. The dish was sealed with vinyl tape and incubated at 27°C for 4 days.…”

“…In order to advance our project for practical application, we screened the vapor activity of 72 essential oils against a T. mentagrophytes, using the box vapor assay reported in a previous study. 12 The vapor activity was compared with the oils' contact activity, determined by an agar diffusion assay.…”

“…12 The box vapor assay against T. mentagrophytes TIMM2789 was carried out according to the procedure reported previously. 12 Three agar plugs implanted with a T. mentagrophytes at 10 7 cells/ml were placed, with the mycelial side on the top, on a petri dish, and placed in an airtight box (1.3-l air capacity). A twofold dilution series of an essential oil or pure compound dissolved in ethyl acetate was soaked uniformly on a filter paper (150 mm in diameter) and placed on the top of the box.…”