Evaluation of the effect of topical and systemic ozone application in periodontitis: an experimental study in rats

Abstract

Objective:The goal of the present study was to determine the effect of systemic and topical ozone application on alveolar bone loss (ABL) by evaluating the effect of Hypoxia-inducible factor −1 alpha (HIF-1-α) and receptor activator of NF-kB ligand (RANKL)-positive cells on histopathological and immunohistochemical changes in a rat periodontitis model.Methodology:Thirty male Wistar rats were divided into three groups: 1) Group C (control group); 2) Group SO (systemic ozone group) and 3) Group TO (topical ozone group). Experimental periodontitis was induced with a 3/0 silk suture placed at the mandibular left first molars of rats, and the suture was removed 14 days later. Ozone gas was injected intraperitoneally (0.7 mg/kg) in SO group. Topical ozone application protocol was performed using an ozone generator at 80% concentration (4th grade) 90- degree probe for the duration of 30 s. Both ozone applications were carried out for two weeks at intervals of two days. Histomorphometric and immunohistochemical analysis were performed.Results:ABL was significantly lower in Group SO compared to Group C (p: 0.0052). HIF-1α- positive cells were significantly lower in Group TO than in Group C (p: 0.0043). RANKL-positive cells were significantly lower in Group SO and in Group TO compared to the control group (p: 0.0033, p: 0.0075, respectively).Conclusion:Both ozone applications decreased RANKL-positive cell counts, TO application decreased HIF-1-α positive cells counts, and SO application was found to be more effective in reducing ABL compared to control group.

Ozônio como coadjuvante na terapia periodontal cirúrgica e não cirúrgica: revisão integrativa da literatura

“…Atualmente, o ozônio tem sido utilizado na indústria alimentícia e na Odontologia como um antisséptico (Leewananthawet et al, 2019). Ele tem um potencial anti-hipóxico, analgésico, é um modulador imunológico (Saglam et al, 2020), além de apresentar um grande poder de oxidação e antimicrobiano por isso, pode ser utilizado como um desinfetante em aplicações odontológicas. Dos estudos selecionados Rapone et al (2020), Alsherif, Eltokhey e Taiema (2020), Leewananthawet et al (2019) e Isler et al (2018) relatam uma forte atividade antimicrobiana do ozônio contra microrganismos periodontais patogênicos anaeróbios, portanto pode ser uma ótima ferramenta complementar à terapia periodontal não cirúrgica em pacientes com periodontite crônica.…”

“…O gás permite que o oxigênio possa se mover para dentro dos tecidos com mais facilidade, aumentando assim a quantidade de 2,3-difosfoglicerato e eritrócitos que também são responsáveis por um aumento nos radicais livres de oxigênio levando a uma mudança na atividade do sistema imunológico e nos níveis de enzimas antioxidantes (Saglam et al,2020), além de benéficos nas células e componentes do sistema imunológico humoral, estimulando a proliferação de células imunocompetentes e síntese de imunoglobulinas. (Dengizek et al, 2018).…”

“…(Dengizek et al, 2018). A terapia com ozônio é capaz de melhorar a oxigenação do tecido afetado e de reduzir o processo inflamatório, portanto, age de forma positiva em algumas doenças infecciosas (Saglam et al, 2020). Além disso, a ozonioterapia pode promover hemostasia, inibir proliferação bacteriana, aumentar liberação de fatores de crescimento e regular positivamente as enzimas antioxidantes celulares (Isler et al, 2018).…”

Periodontal Destruction and Regeneration in Experimental Models: Combined Research Approaches

“…It was concluded, that the adjunctive use of photodynamic therapy in combination with scaling and root planing showed the best therapeutic results in the treatment of periodontal disease in rats after 3 and 15 days [13]. The positive effects of systemic and local ozone application on alveolar bone loss in a Wistar rat periodontitis model and Singlet phototherapy in the treatment of periodontal diseases in an experimental study were determined by Saglam E. et al (2019) and Bazikyan E. A. et al (2018) [27,30]. Radiation therapy and protective melatonin administration produced noticeable biochemical and histochemical effects on periodontal tissues in rats with experimental periodontitis in Kose O. et al (2017) study.…”

“…The results obtained were the subject of histomorphomorphological/-metrical [10, 13, 14, 17, 19, 20, 21-25, 27-29, 30], immunohisto (-cyto)chemical [15,16,10,20,7,28,21,29,6,30,5,19] and histopathological [2,4,5,9,31,22] analysis, micro-computed tomography [9,15,16,18,19,21,24,28]. The laboratory methods involved were real-time quantitative reverse-transcription polymerase chain reaction [11,15,16,21] enzyme-linked immunosorbent assay [16,17], scanning [3], and transmission electron microscopy [32], polarizing light microscopy [29], confocal microscopy and spectrophotometry [15], radiographic and biomechanical analysis [18], descriptive histology and computer-assisted image analysis [12].…”