Evaluation of the effect of temperature and time of incubation on complete blood count (CBC) tests

Obeidi, Narges; Safavi, Eisa; Emami, Habib

doi:10.5897/ajb11.2012

Cited by 3 publications

(3 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Banyak penelitian yang telah dilakukan dalam penundaan pemeriksaan kadar hemoglobin yaitu Evaluasi pengaruh suhu dan waktu inkubasi pada tes hitung darah lengkap (CBC) [14]. Perbedaan darah K3EDTA yang segera diperiksa dan ditunda 2,5 jam pada suhu kamar terhadap kadar hemoglobin dipuskesmas suranenggala [3].…”

Section: Pendahuluanunclassified

“…Penelitian ini sejalan dengan penelitian ini, obeidi (2012) juga menyatakan bahwa Tidak ada perbedaan antara kadar hemoglobin segera diperiksa dan penundaan 24 jam pada suhu ruang [14]. Menurut Rautaray (2017) juga menyatakan bahwa tidak adanya perbedaan kadar hemoglobin segera diperiksa dan penundaan 24 jam pada suhu ruang dan menurut Chairunnisa (2018) menyatakan bahwa tidak terdapat perbedaan kadar hemoglobin segera diperiksa dan penundaan 6 jam pada suhu ruang [18].…”

Section: Pembahasanunclassified

See 1 more Smart Citation

The Difference In Hemoglobin Levels Is Checked Immediately And A Delay Of 2.5 Hours At Room Temperature

Sari

Karlina

Bastian

2023

j jhast

View full text Add to dashboard Cite

Hemoglobin is a protein molecule in red blood cells that functions as a means of transporting oxygen (O2) from the lungs to tissues throughout the body and taking carbon dioxide (CO2) from the tissue below to the lungs to be released into the free air. This study aims to determine the effect between blood immediately examined and a delay of 2.5 hours at room temperature on hemoglobin levels conducted at IKesT Muhammadiyah Palembang. The type of research used is cross sectional with intact group comparison research design. The sample consisted of 30 samples immediately examined and 30 samples with a delay of 2.5 hours taken from 30 respondents. The study was conducted starting from patient preparation, taking examination materials, processing examination materials, analysis and research results. The average hemoglobin level immediately examined was 12.8 g/dL while the hemoglobin level with a delay of 2.5 hours was 12.7 g / dL, the results of the wilxocon test showed that the significant value was p=0.342. The p value obtained is p>0.05. The results can be concluded that there is no difference in hemoglobin levels immediately examined and a delay of 2.5 hours at room temperature.

show abstract

Section: Pendahuluanunclassified

Section: Pembahasanunclassified

The Difference In Hemoglobin Levels Is Checked Immediately And A Delay Of 2.5 Hours At Room Temperature

Sari

Karlina

Bastian

2023

j jhast

View full text Add to dashboard Cite

show abstract

“…Banyak penelitian yang telah dilakukan dalam penundaan pemeriksaan kadar hemoglobin yaitu (Obeidi et al, 2012),evaluasi pengaruh suhu dan waktu inkubasi pada tes hitung darah lengkap (CBC). (Muslim, 2015b)), dalam penelitiannya menunjukan hasil ada pengaruh bermakna penundaan waktu 1 jam, 2 jam dan 3 jam darah K2EDTA dan Na2EDTA terhadap kadar hemoglobin metode Cyanmethemoglobin.…”

unclassified

Perbedaan Hasil Pemeriksaan Kadar Hemoglobin Sampel Darah Vena Remaja Segera Diperiksa dan Ditunda dengan Metode Sahli

Riki Rinaldi,

Silvia Indra,

Tasya Nadira Annisa

2023

JTKB

View full text Add to dashboard Cite

Darah merupakan komponen penting bagi tubuh yang terdiri dari eritrosit, leukosit, trombosit yang dapat digunakan sebagai diagnosis suatu penyakit. Salah satu pemeriksaan laboratorium yang menggunakan darah adalah hemoglobin. Sampel darah yang akan diperiksa harus dilakukan pengecekan terlebih dahulu, karena darah yang disimpan terlalu lama dapat menyebabkan eritrosit pecah dan menyebabkan hemoglobin berada di plasma. Pemeriksaan hemoglobin ada dua cara yaitu manual dan otomatis. Cara manual bisa menggunakan metode sahli. Tujuan penelitian ini adalah untuk mengetahui hasil kadar hemoglobin pada sampel darah vena remaja yang segera diperiksa dan ditunda selama 60 menit, 120 menit pada suhu ruang dengan metode sahli. Penelitian ini menggunakan jenis penelitian eksperimen dengan pendekatan kuantitatif dan uji statistic One Way Anova dengan menggunakan sampel sebanyak 15 remaja. Setiap pemeriksaan dilakukan sebanyak enam kali ulangan. Hasil penelitian ini menunjukkan bahwa adanya perbedaan pemeriksaan segera dan ditunda (60 menit, 120 menit) dikarenakan terjadiadanyapenurunankadar hemoglobin sebanyak 1gr/dL setiap 60 menit dan pada hasi lone way anova menunjukkan hasil penilaian yang signifikan yaitu dengan p 0,00027. Nilai rata-rata hasilkadar hemoglobin menunjukkan pada segera 12,43 gr/dL, ditunda 60 menit 11,48 gr/dL, dan ditunda 120 menit 10,35 gr/dL. Nilai rata-rata kadar hemoglobin remaja laki-laki pada segera (1 3,6 gr/dl), ditunda 60 menit (12,6gr/dL),dan ditunda 120 menit s (11,3 gr/dL). Nilai rata-rata kadar hemoglobin remaja perempuan pada pemeriksaan segera (11,8 gr/dL), ditunda 60 menit (10,9 gr/dL), ditunda 120 menit (9,8 gr/dL).

show abstract

Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

Unalli¹,

Özarda²

2021

J Med Biochemistry

View full text Add to dashboard Cite

Background: The complete blood count (CBC) with differential leukocyte count (DLC) is one of the most common tests requested by physicians. The results of this test are affected by storage temperature and time of incubation. This study was designed to evaluate the stability of hematologic parameters in blood specimens stored for 48 hours at three temperatures. Methods: K2 EDTA- blood was collected from 22 apparently healthy adults. The CBC was performed using Cell-Dyn 3700 hematology analyzer (Abbott Diagnostics, IL, USA) immediately; 0 Time Point and at 4, 8, 12, 16, 20, 24 and 48 h after storage at 4°C, 10°C or 23°C. Changes in values of CBC parameters from the 0 Time Point were determined and reported as % of the initial value. Statistical comparisons were made using the paired-t test and the repeated measures ANOVA. Results: RBC, PLT, Hb and MCH were found stable during 48 h storage at 4°C, 10°C or 23°C. HCT and MCV increased, while WBC decreased significantly at 48 h when stored at 23°C. LYM, NEU, EOS and BASO values showed significant differences after 12 h of storage at 23°C. WBC, HCT, MCV, MCHC, RDW, NEU, MONO, BASO and EOS were stable for 48 h if blood samples were stored at 4°C or 10°C. Conclusions: RBC, PLT, Hb and MCH are the only suitable parameters without refrigeration during 24 h storage. When CBC and DLC are performed, 4°C can be recommended as the most suitable storage temperature for 12 h storage.

show abstract

Evaluation of the effect of temperature and time of incubation on complete blood count (CBC) tests

Cited by 3 publications

References 11 publications

The Difference In Hemoglobin Levels Is Checked Immediately And A Delay Of 2.5 Hours At Room Temperature

The Difference In Hemoglobin Levels Is Checked Immediately And A Delay Of 2.5 Hours At Room Temperature

Perbedaan Hasil Pemeriksaan Kadar Hemoglobin Sampel Darah Vena Remaja Segera Diperiksa dan Ditunda dengan Metode Sahli

Stability of hematological analytes during 48 hours storage at three temperatures using Cell-Dyn hematology analyzer

Contact Info

Product

Resources

About