Evaluation of the effect of Withania somnifera root extracts on cell cycle and angiogenesis

Mathur, Rajani; Gupta, Suresh; Singh, Neeta; Mathur, Sandeep; Kochupillai, V.; Velpandian, Thirumurthy

doi:10.1016/j.jep.2005.11.020

Cited by 89 publications

(56 citation statements)

References 21 publications

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“…First, withanolides induce growth arrest and apoptosis in cancer cells (4,5). Second, withanolides can inhibit angiogenesis by suppressing endothelial cell proliferation (6). Third, withanolides can reduce cancer cell invasion and metastasis (7).…”

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confidence: 99%

Tubocapsanolide A Inhibits Transforming Growth Factor-β-activating Kinase 1 to Suppress NF-κB-induced CCR7

Pan

Chang

et al. 2009

Journal of Biological Chemistry

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Withanolides are steroidal lactones that were originally isolated from Withania somnifera, one of the most important herbs used as a traditional remedy for several illnesses in Asian countries (1). These compounds are biologically active and have been shown to inhibit the enzymatic activity of cyclooxygenase-2 and suppress inflammation (2). In addition, recent studies demonstrate that withanolides exhibit anti-cancer effect on human lung, colon, and breast cancer cells in vitro and exert immunopotentiating activity in vivo (3, 4). Several potential mechanisms have been implicated in the inhibition of tumorigenesis by withanolides. First, withanolides induce growth arrest and apoptosis in cancer cells (4, 5). Second, withanolides can inhibit angiogenesis by suppressing endothelial cell proliferation (6). Third, withanolides can reduce cancer cell invasion and metastasis (7). These data suggest that withanolides may be developed as a novel class of anti-cancer drugs.Lymph node invasion by cancer cells is an important step for tumor metastasis and is frequently correlated with early recurrence and poor prognosis. However, the underlying mechanism by which cancer cells metastasize into peripheral lymphatic capillaries is poorly defined. Recent studies indicate that the interaction between chemokine CCL21 and its cognate receptor CCR7 may play an important role in this process (8, 9). The hypothesis suggests that lymphatic endothelial cells (LECs) 2 express and release chemotactic factors such as CCL19 and CCL21 to direct cancer cells with high expression of chemokine receptor CCR7 to grow and migrate toward lymphatic capillaries (10 -12). Indeed, many highly metastatic cancer cells express large amount of CCR7 receptor (13,14), and CCR7 expression is associated with lymph node metastasis in breast, lung, gastric, esophageal cancer, and melanoma (15-19). It is possible that inhibition of CCR7 expression or block of CCL21-CCR7 interaction may cause reduction of lymph node invasion and tumor metastasis. However, the control of CCR7 gene transcription in cancer cells is largely unclear, and no natural products have been shown to regulate CCR7 expression.We have isolated a new bioactive withanolide tubocapsanolide A (Tubo A) from Tubocapsicum anomalum (20). Tubo A exhibited cytotoxic activity on various types of human cancer cells (20). In addition, our recent results demonstrated that Tubo A suppressed the transcription of Skp2 oncogene and up-regulated cyclin-dependent kinase inhibitory proteins p27

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confidence: 99%

Tubocapsanolide A Inhibits Transforming Growth Factor-β-activating Kinase 1 to Suppress NF-κB-induced CCR7

Pan

Chang

et al. 2009

Journal of Biological Chemistry

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“…The previous studies showed that the plant leaves contain calotropin and calotropegnin while the latex contain uzarigenin, and terpenol ester, in addition Calotropis procera contain cardiac glycosides and many other compounds that have different pharmacological activity including insecticidal, antifungal and anticancer (Ahmed et al, 2006), analgesic activity of dry latex (Saber et al, 1969), antifertility activity ethanolic extract of the roots (Saxena and Saxena, 1979) antitumor potential of the root extracts (Mathur et al, 2009), anthelmintic of the flowers (Larhsini et al, 1997). This study aims to test some medicinal potentials and biological activity of crude aqueous, chloroform and ethanolic extract of Calotropis procera leaves in addition to its latex.…”

Section: Discussionmentioning

confidence: 99%

Biochemical effects of Calotropis procera on hepatotoxicity

Alrheam

2015

Biomed Res Ther

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Abstract-Introduction:Calotropis procera commonly known as 'Sodom apple' is a 6-meter high shrub that belongs to the Asclepiadaceae plant family and is commonly found in West Africa and other tropical places. In Saudi Arabia the plant is commonly used in traditional medicine for the treatment of variety of diseases including fever, constipation, muscular spasm and joint pain. Aim: In the present study C. procera were investigated for the hepatoprotective activity. Material and Methods: Carbon tetrachloride is used to produce hepatotoxicity. Forty-two male albino rats, weighting 150-200 gm divided into seven groups, each consisted of 6 rats. Carbon tetrachloride 2 ml/kg was administered twice a week to all of the groups of animals except group I, which served as control and given the normal saline. Group II served as carbon tetrachloride control. Group III received Silymarin at 100 mg/kg/day dose, Group IV received aqueous leaves extracts C. procera 200 mg/kg, Group V received chloroform leaves extracts C. procera 200 mg/kg, Group VI received ethanol leaves extracts C. procera 200 mg/kg, Group VII received latex of C. procera 200 mg/kg. The effect of aqueous, chloroform, ethanol leaves extract and latex C. procera on biochemical parameters of liver was measured. Results: The results showed that the aqueous, chloroform, ethanol leaves extract and latex C. procera produced significant decrease in acid phosphatase, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, total protein, albumin and total bilirubin levels compared to the CCL 4 treated group II. Conclusion: Calotropis procera appears to have hepatoprotective activity and these may be due to enrich of the plant by phytoconstituents that activate and hence a pharmacological response of different parts of the body and this study need further studies to show the complete properties of the plant.

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“…Jung et al, 16 have studied the effect of medicinal plant extract on swimming capacity of spermatozoa of mice. Mathur et al, 4 have observed the effect of Withania somnifera root extracts on cell cycle and angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

“…The Indian System of Medicine, viz Ayurveda, Siddha, Unani and Homeopathic system predominantly use plant-based raw materials for most of their preparations and formulations. Ashwagandha (Withania somnifera) is a medicinal plant extensively used in Ayurveda as home remedy for various diseases 4 .…”

Section: Introductionmentioning

confidence: 99%

Untitled

2012

IJPSR

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Background/Aim: Endosulfan is a pesticide of organochlorine group uses by 55 % of farmers in Bihar. Present study aims to illustrate effect of Endosulfan on biochemical, hormonal and sub cellular anomalies of spermatozoa of mice and their restoration through root extract of Withania somnifera. Materials and Methods: Experimental mice were administered with endosulfan for eight weeks followed by eight weeks administration of Withania somnifera. Results: Endosulfan administered group show degenerated mitochondria and plasmamembrane. Degenerated microtubule were also observed with rudimentary central hub and dynein arm. While eight weeks Withania somnifera 1000 mg/kg/b.w/day administered group show greater degree of sub cellular restoration on mitochondria and nuclear membrane. Microtubules were almost normal in structure. Lipid peroxidation level were also restored toward normal after ashwagandha administration. Conclusion: These combined effect finally leading to restoration in structure of spermatozoa in mice. This is very effective in restoring male fertility by combating endosulfan toxicity.

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Evaluation of the effect of Withania somnifera root extracts on cell cycle and angiogenesis

Cited by 89 publications

References 21 publications

Tubocapsanolide A Inhibits Transforming Growth Factor-β-activating Kinase 1 to Suppress NF-κB-induced CCR7

Tubocapsanolide A Inhibits Transforming Growth Factor-β-activating Kinase 1 to Suppress NF-κB-induced CCR7

Biochemical effects of Calotropis procera on hepatotoxicity

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