Evaluation of the Effects of Water Disinfection By-products, Bromochloroacetic and Dibromoacetic Acids, on Frog Embryogenesis

Weber, Naomi; Higuchi, Ty; Tessari, John D.; Veeramachaneni, D. N. Rao

doi:10.1080/15287390490443713

Cited by 8 publications

(3 citation statements)

References 33 publications

(42 reference statements)

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“…For instance, the 96-h LC50 and EC50 for bromochloroacetic acid were between 10,000 and 12,000 ppm, while dibromoacetic acid did not produce any significant mortality or malformation even at 16,000 ppm during a FETAX (Weber et al, 2004). Similarly, Dawson et al (1989) observed terata in Xenopus laevis embryos after a 96-h exposure to 6-aminonicotinamide, isoniazid, or urethane, but not after exposure to nitrilotriacetate or cyclamate.…”

Section: Discussionmentioning

confidence: 82%

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Exposure to Low Concentrations of Di-n-butyl Phthalate During Embryogenesis Reduces Survivability and Impairs Development of Xenopus Laevis Frogs

Lee

Owens

Veeramachaneni

2005

Journal of Toxicology and Environmental Health, Part A

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Di-n-butyl phthalate (DBP) appears to be a ubiquitous environmental contaminant, as indicated by its presence in air, water, and soil worldwide (Giam et al., 1980; ATSDR, 2001; Peterson & Freeman, 1982) and the presence of its major metabolite, monobutyl phthalate (MBP), in random human urine samples (Blount et al., 2000). Studies indicate that exposure to a variety of endocrine-disrupting chemicals, such as DBP, may be partially responsible for reported global amphibian declines; if so, amphibians may serve as ecological harbingers for the future of human health. Therefore, this study was undertaken to investigate the effects of environmentally relevant concentrations of DBP on development in Xenopus laevis African clawed frogs. Developmental effects of DBP on Xenopus embryos were determined using the 96-h frog embryo teratogenesis assay-Xenopus (FETAX). Embryos (n = 300/group) were exposed from gastrulation (stage 8-11) through primary organogenesis (stage 46) to 0.1, 0.5, 1, 5, 10, or 15 ppm DBP dissolved in 0.01% dimethyl sulfoxide (DMSO), vehicle alone (0.01% DMSO; solvent control), or FETAX culture medium only (control; n = 600). At 96 h, mortalities for control, solvent control, and 0.1, 0.5, 1, 5, 10, and 15 ppm DBP were 5, 4, 6, 5, 5, 9, 18, and 52%, respectively; the incidence of developmental malformations in the surviving tadpoles was 7, 9, 15, 37, 51, 53, 90, and 100%. The average length of embryos was significantly lower in all DBP treatment groups. Thus, DBP significantly affected development of Xenopus embryos at low, environmentally relevant concentrations.

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Section: Discussionmentioning

confidence: 82%

“…Studies using these modifications to FETAX have been reported from our laboratory (Weber et al, 2004).…”

Section: Experimental Designmentioning

confidence: 98%

Exposure to Low Concentrations of Di-n-butyl Phthalate During Embryogenesis Reduces Survivability and Impairs Development of Xenopus Laevis Frogs

Lee

Owens

Veeramachaneni

2005

Journal of Toxicology and Environmental Health, Part A

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“…The hepatotoxicities of dibromoacetate (DBA), bromochloroacetate (BCA), and bromodichloroacetate (BDCA) were indicated in one study in mice, and in another with regard to their abilities to induce 8‐OH‐dG in hepatic nuclear DNA of mice . However, other studies mainly investigated the potentials of the compounds for the production of developmental toxicity, genotoxicity in Salmonella typhimurium strain TA100, and cell cytotoxicity and genotoxicity in Chinese Hamster Ovary (CHO) cells …”

Section: Introductionmentioning

confidence: 99%

Comparative toxicity studies on bromochloroacetate, dibromoacetate, and bromodichloroacetate in J774A.1 macrophages: Roles of superoxide anion and protein carbonyl compounds

Hassoun

Zeng

2018

J Biochem & Molecular Tox

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The brominated and mixed bromo-chloro-haloacetates, such as dibromoacetate (DBA), bromochloroacetate (BCA), and bromodichloroacetate (BDCA), are by-products of water chlorination and are found at lower levels than the fully chlorinated acetates in the drinking water. The toxicities of the compounds were assessed in J774A.1 cells and were found to induce concentration-dependent increases in cell death and superoxide anion and protein carbonyl compounds production. Compared to the previously tested concentrations of dichoroacetate (DCA) and trichloroacetate (TCA) in the same cell line, the tested haloacetates induced similar effects on cellular viability and superoxide anion production but at DBA and BCA concentrations that were approximately 40-160 times lower than those of DCA and TCA, and at BDCA concentrations that were 4-16 times lower than those of DCA and TCA. Also, production of super oxide anion, protein carbonyl compounds, and induction of phagocytic activation are suggested to play a role in their toxicity.

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Formation potentials of bromate and brominated disinfection by-products in bromide-containing water by ozonation

Lin

Chen

2014

Environ Sci Pollut Res

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The ozonation involved in drinking water treatment raises issues of water quality security when the raw water contains bromide (Br(-)). Br(-) ions may be converted to bromate (BrO3 (-)) during ozonation and some brominated disinfection by-products (Br-DBPs) in the following chlorination. In this study, the effects of ozone (O3) dosage, contact time, pH, and Br(-) and ammonia (NH3-N) concentrations on the formation of BrO3 (-) and Br-DBPs have been investigated. The results show that decreasing the initial Br(-) concentration is an effective means of controlling the formation of BrO3 (-). When the concentration of Br(-) was lower than 100 μg/L, by keeping the ratio of O3 dosage to dissolved organic carbon (DOC) concentration at less than 1, BrO3 (-) production was effectively suppressed. The concentration of BrO3 (-) steadily increased with increasing O3 dosage at high Br(-) concentration (>900 μg/L). Additionally, a longer ozonation time increased the concentrations of BrO3 (-) and total organic bromine (TOBr), while it had less impact on the formation potentials of brominated trihalomethanes (Br-THMFP) and haloacetic acids (Br-HAAFP). Higher pH value and the presence of ammonia may lead to an increase in the formation potential of BrO3 (-) and Br-DBPs.

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Evaluation of the Effects of Water Disinfection By-products, Bromochloroacetic and Dibromoacetic Acids, on Frog Embryogenesis

Cited by 8 publications

References 33 publications

Exposure to Low Concentrations of Di-n-butyl Phthalate During Embryogenesis Reduces Survivability and Impairs Development of Xenopus Laevis Frogs

Exposure to Low Concentrations of Di-n-butyl Phthalate During Embryogenesis Reduces Survivability and Impairs Development of Xenopus Laevis Frogs

Comparative toxicity studies on bromochloroacetate, dibromoacetate, and bromodichloroacetate in J774A.1 macrophages: Roles of superoxide anion and protein carbonyl compounds

Formation potentials of bromate and brominated disinfection by-products in bromide-containing water by ozonation

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