Evaluation of the Effects of Endodontic Materials on Fibroblast Viability and Cytokine Production

Gomes‐Filho, João Eduardo; Watanabe, Simone; Gomes, Alessandra Rodrigues; Faria, Max Douglas; Lodi, Carolina Simonetti; Oliveira, Sandra Helena Penha de

doi:10.1016/j.joen.2009.07.022

Cited by 46 publications

(58 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Calcium hydroxide sealers are generally known to have good cytocompatibility 19,20) . Regarding Sealapex, our results were not in agreement with those of previous studies, which showed that this material was noncytotoxic at 24 h after mixing 21) . In the present study, Sealapex exhibited a high cytotoxic effect on MG-63 cells and human gingival fibroblasts and retained its cytotoxicity throughout the observation period of 7 days.…”

Section: Discussioncontrasting

confidence: 99%

“…The higher cytotoxicity of Sealapex can be attributed to the difference in the volume of sealers and medium used. In this study, each well containing 0.1 cc set sealer received 200 µL of DMEM for preparation of the eluate of the sealer, whereas in the other published studies 20,21) the eluates of the sealers were prepared by placing the sealer material in a greater amount of culture medium. When preparing the eluates of the sealers, the sealers were mixed and allowed to set at 37°C in 100% relative humidity for 24 h. However, Sealapex did not set after 1 day, and it was reported that Sealapex sets in 2 to 3 weeks in 100% relative humidity 22) .…”

Section: Discussionmentioning

confidence: 99%

“…Sealers based on MTA have been reported to be biocompatible, to stimulate mineralization 21) , to encourage apatite-like crystalline deposits along the apical and middle thirds of the canal walls 26) . EndoSeal showed the most favorable effect on both types of cells compared to AH Plus and Sealapex possibly due to the superior biological properties of MTA.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim

Shin

2014

Dent. Mater. J.

View full text Add to dashboard Cite

The objective of this study was to assess the cytotoxicity of EndoSeal, a novel mineral trioxide aggregate-based root canal sealer in comparison with two commonly used sealers, AH Plus and Sealapex. For cytotoxicity assay, MG-63 cells and human gingival fibroblasts were incubated in culture medium containing eluates of each sealer at 1, 3, and 7 days. Cell metabolism was evaluated by the WST-1 assay. For cell adhesion assay, disc specimens were fabricated from EndoSeal and AH Plus. MG-63 cells and human gingival fibroblasts were seeded on the discs, and after 1 day and 7 days of incubation, cell morphology and cell adhesion were examined by SEM. The order of cytotoxicity of root canal sealers was as follows: EndoSeal < AH Plus < Sealapex. Both types of cells seeded on the EndoSeal specimens were much larger, flat with rough margins compared to those on the AH Plus specimens. These results suggest that EndoSeal has a satisfactory cytocompatibility.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Kim

Shin

2014

Dent. Mater. J.

View full text Add to dashboard Cite

show abstract

“…Endodontic treatment aimed to restore the normality of apical and periapical tissues [1][2][3][4] through a root canal system deep cleaning and disinfection, to control pathogenic microorganisms and the complete three-dimensional sealing using root canal sealers [5][6][7]. To achieve this goal, irrigation and aspiration of the root canal system, coupled with instrumentation, composes an important stage of radical endodontic treatment, the chemical mechanical preparation.…”

Section: Introductionmentioning

confidence: 99%

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

Martins¹

2017

J. Dent. Sci. Ther

View full text Add to dashboard Cite

The aim of this study was to evaluate the ethanolic extract of Psidium cattleianum biocompatibility by structural and functional cell viability maintenance. Cell viability was analyzed after 1 h, 3 h, 6 h, 10 h and 24 h of incubation by Exclusion Method with trypan blue and MTT assay, using human mononuclear cells (PBMC) and human periodontal ligament (PDL) cells in culture. Positive control used was RPMI cell culture medium and negative control was ethanol 80%. The obtained data were subjected to statistical analysis at a significance level of 5%. By trypan blue assay, ethanolic extract presented a homogeneous performance of PBMC from the 1 h, with average viability between 30 and 50% (p>0.05). By MTT assay, the results showed the efficacy of the ethanolic extract of Psidium cattleianum in maintaining the functional viability of the periodontal ligament cells to 10 h (p>0.05), presenting values greater than the RPMI control. In both methods, it can be observed that the extract properties can neutralize the cytotoxic effect of its diluent ethanol 80%, which showed average viability nearly zero (p <0.05). Thus, biocompatibility by structural and functional cell viability maintenance was considered acceptable to ethanolic extract of Psidium cattleianum and further studies should be performed to indicate its use as an alternative to irrigation solution traditionally used in everyday practice.

show abstract

“…Endodontic treatment expected to reestablish the typicality of apical and periapical tissues [1][2][3][4] through a root channel framework profound cleaning and cleansing, keeping in mind the end goal to control pathogenic microorganisms and the entire three-dimensional fixing utilizing root trench sealers [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

MTT Assay of Psidium cattleianum

Martinz¹,

John²,

Hidalgo³

2017

Periodon Prosthodon

View full text Add to dashboard Cite

The point of this review was to assess the ethanolic concentrate of Psidium cattleianum biocompatibility by basic and utilitarian cell reasonability support. Cell practicality was broke down after 1, 3, 6, 10 and 24 hours of hatching by Exclusion Method with trypan blue and MTT measure, utilizing human mononuclear cells [PBMC] and human periodontal tendon [PDL] cells in culture. Positive control utilized was RPMI cell culture medium and negative control was ethanol 80%. The got information were subjected to measurable examination at a criticalness level of 5%. By trypan blue examine, ethanolic remove displayed a homogeneous execution of PBMC from the 1 h, with normal reasonability in the vicinity of 30 and half (p>0.05). By MTT examine, the outcomes demonstrated the adequacy of the ethanolic concentrate of Psidium cattleianum in keeping up the utilitarian reasonability of the periodontal tendon cells to 10 h (p>0.05), displaying values more noteworthy than the RPMI control. In both techniques, it can be watched that the concentrate properties can kill the cytotoxic impact of its diluent ethanol 80%, which indicated normal practicality almost zero (p<0.05). The biocompatibility by auxiliary and useful cell feasibility support was viewed as satisfactory to ethanolic concentrate of Psidium cattleianum and further reviews ought to be performed to demonstrate its utilization as another option to water system arrangement generally utilized as a part of regular practice.

show abstract

Evaluation of the Effects of Endodontic Materials on Fibroblast Viability and Cytokine Production

Cited by 46 publications

References 35 publications

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Cytotoxicity of a novel mineral trioxide aggregated-based root canal sealer

Ethanolic Extract of Psidium cattleianum as Intracanal Irrigation: An In vitro Study of Cell Viability

MTT Assay of Psidium cattleianum

Contact Info

Product

Resources

About