Evaluation of the Efficiency of Combined and Separated Antioxidant Supplementation of Vitamin C and E on Semen Parameters in Streptozotocin-Induced Diabetic Male Wistar Rats

Abstract

Male reproductive functions could be affected at multiple levels due to dia-betes mellitus. Antioxidants vitamins have direct impact on improving male reproductive capacity. Our objective was to assess the efficiency of treat-ment with vitamin C and E in the case of whether diabetes mellitus is the cause of infertility or if infertile men suffer from diabetes by inducing diabe-tes on male Wistar rats. Adult male Wistar rats were divided into 5 groups of 6 animals each: a normoglycemic control. Other four groups were given a single dose of streptozotocin, and divided into: diabetic control, diabetic + 250 mg/kg/day of vitamin C, diabetic + 250 mg/kg/day of vitamin E, diabetic + 250 mg/kg/day of vitamin C + 250 mg/kg/day of vitamin E by gavage during 30 consecutive days. Animals were anesthetized, sacrificed to evaluate body and reproductive organ weights, plasma hormone levels, and semen quality. Compared to normoglycemic animals, diabetic rats showed reduced body weight (211.33 ± 8.70gr) and epididymis (1.35 ± 0.10 gr). The testis weight (3.10 ± 0.14 gr) and sperm count (2.72 ± 4.61 x 106) were maintained. A reduction of LH and testosterone levels (0.10 ± 0.00, and 0.25 ± 0.12) respec-tively. There was a significant increase (p<0.05) in the final body and epididy-mis weights, hormonal levels, and sperm count among diabetes vitamins treated groups as compared to the normoglycemic and hyperglycemic groups. Diabetes mellitus induces adverse effects on reproductive capacity. Antioxidant vitamins C and E improves sperm quality and increases hormonal levels.

Polyphenols contents and antioxidant potential of Nauclea latifolia Smith (Rubiaceae) acetonic fractions from Burkina Faso

“…This drug is available as a synthetic analgesic since the 1970s. It is also known to have a strong antibacterial property 16,18 . Accordingly, it could be interesting to continue the investigating of this plant to ascertain and validate its medicinal value with a view to discovering new leads for better and improved management of infectious disease conditions.…”

Effects of Vitamin C on reproductive parameters of rabbit bucks with experimentally induced metronidazole toxicity

“…This is similar to the finding of Farombi et al (2008) who observed improvement in the testicular and epididymal weight of rats affected by tetracyclineinduced reproductive toxicity. Similarly, Benabbou et al (2017) also found out that vitamin C was helpful in restoring decreased testicular weight in wistar rats. The result on sperm motility showed a significant (P ≤ 0.05) decrease in the percentage sperm motility in the metronidazole group which IV).…”

Protective effects of vitamin C on hormonal level and testicular histopathology of rabbit bucks with metronidazole-induced toxicity

“…Additionally, it has been observed to elevate blood testosterone levels (Fernandes et al, 2011;Sonmez et al, 2005). According to a study conducted by Benabbou et al (2017), the administration of Vitamin C has been found to have beneficial effects in mitigating the adverse effects of different drugs and substances on the reproductive system. Furthermore, it has been demonstrated in a study conducted by Sadeghzadeh et al (2018) that vitamin C has the ability to mitigate the detrimental impacts of dexamethasone on sperm motility, testosterone levels, and spermatogenesis indexes in mice.…”