Man has used medicinal plants for a long time. Among these Thymus vulgaris whose therapeutic virtues have been proven by many works, especially in the field of the fight against infectious diseases. The objective of this work is to evaluate the effect of essential oils, obtained by extraction from a medicinal plant Thymus vulgaris on the growth of Helicobecter Pylori. Various pro-cesses for extracting T. vulgaris essential oil from the Tessala region of SBA (Western Algerian) have been highlighted. Quantitative analysis of T. vulgaris essential oil after its hydrodistillation extraction showed a good yield of 3.2%.The study of the antibacterial effect of T. vulgaris essential oil against the H. pylori taken from gastric biopsies (cases of gastritis, ulcer, malignant tumor) within the Gastroenterology Department at the Hospital University Centre of SBA, was highlighted by the aromatogram test, the MIC and MBC test and the test of monitoring bacterial growth in the absence and presence of essential oil. The aromatogram test of H. pylori in the presence of T. vulgaris essential oil allowed us to test several inhibitory concentrations (10-1 to 10-5). The results of the aromatogram obtained showed a good activity of T. vulgaris essential oil in comparison with H. pylori. In all the three pathological cases (gastric malignant tumours, gastritis and ulcer). The results of the MIC and MBC tests allowed us to determine the minimum inhibitory concen-tration and that of bactericidal, whose highest recorded inhibition values of T. vulgaris essential oil in relation to H. pylori in all the three above stated pathological cases are (62.5, 125) μ.ml-1respectively.

Male reproductive functions could be affected at multiple levels due to dia-betes mellitus. Antioxidants vitamins have direct impact on improving male reproductive capacity. Our objective was to assess the efficiency of treat-ment with vitamin C and E in the case of whether diabetes mellitus is the cause of infertility or if infertile men suffer from diabetes by inducing diabe-tes on male Wistar rats. Adult male Wistar rats were divided into 5 groups of 6 animals each: a normoglycemic control. Other four groups were given a single dose of streptozotocin, and divided into: diabetic control, diabetic + 250 mg/kg/day of vitamin C, diabetic + 250 mg/kg/day of vitamin E, diabetic + 250 mg/kg/day of vitamin C + 250 mg/kg/day of vitamin E by gavage during 30 consecutive days. Animals were anesthetized, sacrificed to evaluate body and reproductive organ weights, plasma hormone levels, and semen quality. Compared to normoglycemic animals, diabetic rats showed reduced body weight (211.33 ± 8.70gr) and epididymis (1.35 ± 0.10 gr). The testis weight (3.10 ± 0.14 gr) and sperm count (2.72 ± 4.61 x 106) were maintained. A reduction of LH and testosterone levels (0.10 ± 0.00, and 0.25 ± 0.12) respec-tively. There was a significant increase (p<0.05) in the final body and epididy-mis weights, hormonal levels, and sperm count among diabetes vitamins treated groups as compared to the normoglycemic and hyperglycemic groups. Diabetes mellitus induces adverse effects on reproductive capacity. Antioxidant vitamins C and E improves sperm quality and increases hormonal levels.

Antibiotic resistance is currently a major public health problem facing hospitals around the world, another concern and that of the increased risks of additive synthetic food, added to foods that objective is to preserve foodstuffs. All these parameters are behind the urgent need for a natural antimicrobial agent. Rosmarinus officinalis L. is used in traditional medicine as an antispasmodic, ingredients in beauty products and so good in the preservation of food products. In this context, the objective of this study is to evaluate the antimicrobial activity of essential oils extracted from two species of the same genus of rosemary of wild origin "Rosmarinus eriocalyx" and "Rosmarinus officinalis" of a home garden in the Sidi Ali Benyoub region, against three pathogenic bacteria (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa). Our work is divided into two parts, the first consists of extracting a volume of essential oil from 100g of each plant species by the hydrodistillation method which is the most effective and the most useful one, the second part is based on the evaluation of the effect of these oil extracts on the growth of the three pathogenic bacteria "Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa" taken from a biological sample "the urine" in the presence of a urinary tract infection at the level of the Central Laboratory, bacteriology department of the SBA center, which is highlighted by the aromatogram tests and the monitoring of bacterial growth in the absence and in the presence of essential oils. The diffusion method on the agar medium made it possible to determine the diameters of the zones of inhibition. The results show that the quantitative analysis of the essential oils of Rosmarinus eriocalyx and Rosmarinus officinalis L gave good yields of 1% and 0.63%. Regarding the antibacterial activity of both oils against pathogenic bacteria, the results show us that Rosmarinus eriocalyx (wild) has a higher activity compared to "Rosmarinus officinalis L". We can see that these oils have antibacterial activity only against Pseudomonas aeruginosa but the other two bacteria "Staphylococcus aureus and Escherichia coli" can be resistant for these oil extracts.

The extensive use of antibacterial agents in human medication has led to the appearance of resistant microbial strains, hence the importance of directing research towards new molecules, where a good part of scientific research is currently directed towards the way of the use of active biological extracts of medicinal plants, in particular towards essential oils.In this context, the objective of this work is to evaluate the antibacterial activity of essential oils extracted from Mentha pulegium and Laurus Nobilis on some pathogenic bacterial species such as Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa. The extraction of essential oils from aromatic plants: Mentha pulegium, Laurus Nobilis is carried out by the hydrodistillation technique.The results reveal that Mentha pulegium and Laurus Nobilis show a yield of 2.7% and 0.79% respectively. Our results indicate that the two essential oils Mentha pulegium and Laurus Nobilis have very low activity against Escherichia coli, Staphylococci aureus, Pseudomonas aeruginosa, which represent inhibition diameters of 6 mm; 4mm and 2mm respectively. The bacterium Pseudomonas aeruginosais the most resistant to the two essential oils extracted from Mentha pulegium, Laurus Nobilis.

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