Evaluation of the ELISA test for the antibody detection in cattle naturally and experimentally infected with Cysticerccus bovis

Monteiro, Lilian Lameck; Pinto, P.S.A.; Dias, Francesca Silva

doi:10.1016/j.vetpar.2006.05.017

Cited by 14 publications

(14 citation statements)

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“…Estas amostras foram dessoradas à temperatura ambiente e logo em seguida estocadas a -20 o C. O diagnóstico sorológico da cisticercose animal foi realizado por triagem pelo teste ELISA indireto, sendo os casos positivos submetidos ao Immunoblot para confirmação e resultado final (PINTO et al, 2000(PINTO et al, , 2001MONTEIRO;DIAS, 2006). Para o teste de ELISA indireto as placas de poliestireno foram sensibilizadas com antígeno heterólogo, de cisticercos de Taenia crassiceps, que apresenta reação com os anticorpos para cisticercos de Taenia solium e Taenia saginata.…”

Section: Methodsunclassified

Prevalência do complexo teníase-cisticercose na zona rural de Matias Barbosa-MG

et al. 2012

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ResumoO complexo teníase-cisticercose é uma zoonose de grande importância para a saúde pública e animal. O ser humano desempenha importante papel sendo o único hospedeiro definitivo do parasita. Albergando no intestino as formas adultas da Taenia saginata ou da Taenia solium, cabendo à forma larvar (cisticerco) o desenvolvimento na musculatura de bovinos e suínos ou do próprio homem, quando este também atua como hospedeiro intermediário. Visando obter maior conhecimento sobre esta zoonose, o objetivo do presente estudo foi determinar o perfil epidemiológico do complexo teníase-cisticercose na zona rural do município de Matias Barbosa, localizado na Zona da Mata de Minas Gerais. Para tanto, foram realizadas coletas de sangue de 350 bovinos e 81 suínos e amostras de fezes de 124 pessoas da população presente em 74 propriedades rurais. Além disso, foi aplicado um questionário epidemiológico sobre as condições sócio-econômicas e sanitárias de cada propriedade. Observou-se uma prevalência de 0,9% de bovinos positivos ao teste de Immunoblot. Não houve suínos positivos, assim como também não foi diagnosticada pessoa portadora de teníase. Alguns fatores de risco de transmissão do complexo teníase-cisticercose no município foram identificados como o consumo de carne bovina e suína mal aquecida, propriedades sem saneamento básico e criação de bovinos com acesso a fontes de contaminação. Apesar da baixa prevalência da cisticercose bovina encontrada no município de Matias Barbosa, é necessária a tomada de medidas de controle do complexo teníase-cisticercose para impedir que haja aumento de casos da doença no município, tendo em vista que foram registrados fatores favoráveis à sua manutenção. Palavras-chave: Imunodiagnóstico, prevalência, fatores de risco, teníase-cisticercose, zoonose AbstractThe taeniosis-cysticercosis complex is an important disease to the public and animal health. The man plays an important role in the life cycle, because he is the unique definitive host. The adult forms of the Taenia solium or Taenia saginata holds in gut, and the larvae forms develops in the muscle of bovine and swine, respectively. The objective of this study was to determine the prevalence and risk factors of the taeniosis-cysticercosis complex transmission in rural area of Matias Barbosa -MG, Brazil. Catla and swine lood and human stool samples were collected from 74 farms. Cysticercosis were eníasis were diagnosis serological methods and the teniasis by direct microscopy. Moreover, a questionnaire was

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Section: Methodsunclassified

Prevalência do complexo teníase-cisticercose na zona rural de Matias Barbosa-MG

et al. 2012

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“…O diagnóstico sorológico dos bovinos foi realizado por triagem, em triplicata, pelo ELISA indireto e os casos suspeitos (resultados positivos ao ELISA) foram submetidos ao Imunoblot para confirmação, seguindo as metodologias (Pinto et al 2000, Pinto et al 2001, Monteiro et al 2006 …”

Section: Methodsunclassified

Perfil da transmissão e prevalência da cisticercose bovina em propriedades rurais do Triângulo Mineiro

et al. 2016

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Cysticercosis can affect cattle which develop the larvae Taenia saginata. Despite the importance of cysticercosis for public animal health and for the economy, the epidemiological reality of such zoonosis in Brazil is little known. In the southeastern region of the State of Minas Gerais most of the data are obtained from the official Sanitary InspectionServices in slaughterhouses, but little information based on field data from live animals. In order to assess the epidemiological situation of bovine cysticercosis in small and medium-sized rural properties of four municipalities, located in the region of Triângulo Mineiro, we searched the occurrence and the major risk factors associated with transmission of the disease in those properties. We conducted a cross-sectional epidemiological study involving 68 properties located in rural areas of the four municipalities, where there were collected 1002 bovine blood samples and applied an epidemiological questionnaire. The serological diagnosis of cysticercosis was conducted by sorting through indirect ELISA test, and the suspected cases were subjected to Immunoblot for confirmation. This study revealed the prevalence of 4.7% of bovine cysticercosis in rural properties of the municipalities sampled from July to August 2013. The factors which were statistically significant for the transmission of cysticercosis were the expertise of those responsible for the property (OR 5.81 IC 1,), the family income ) and the water quality of the property (OR 9.40 IC 1.97-9.59). Other factors observed were of lower significance. These results identified the main factors which interfere in the transmission of bovine cysticercosis, allowing to subsidize preventive measures and to control this important zoonotic disease in the region of Triângulo Mineiro.

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“…The sera were stored individually in microtubes at -20° C. These sera samples were submitted to an ELISA test with screening, in triplicate, for the diagnosis of cysticercosis (SILVA et al, 2015). The suspected cases (positive result by ELISA) were submitted to immunoblot for confirmation (MONTEIRO et al, 2006). …”

Section: Delineationmentioning

confidence: 99%

Epidemiological aspects of cysticercose in relation to hydrographic net at triangulo mineiro, MG, Brazil

Duarte

Pinto

Silva

et al. 2018

SCA

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Cysticercosis affects cattle, which develop the larva of Taenia saginata after ingesting their eggs, which are released by humans. It is a parasite that is distributed worldwide. The use of geoprocessing allows a better definition of endemic areas and populations that face different levels of risk and thus creates a basis for specific control programs for each farming system. The objective of this study was to evaluate the epidemiological situation of bovine cysticercosis in four municipalities in the Triângulo Mineiro region (Romaria, Iraí de Minas, Grupiara and Douradoquara), with special attention given to the influence of the hydrographic network on the spatial distribution of the disease. The properties sampled (68) for laboratory and epidemiological analysis (questionnaire) were also georeferenced. The risk factors were analyzed using the ArcGIS 10.1 program. The animals were submitted to immunodiagnostic tests (enzyme-linked immunosorbent assay and immunoblot) for the diagnosis of bovine cysticercosis. After confirming the disease focus properties, the spatial distribution of the cases was analyzed in relation to the watershed of the region. The positive results for bovine cysticercosis on the properties of the municipalities of Romaria and Iraí de Minas were grouped together, which was a significant result (p < 0.01). Although the positive results for bovine cysticercosis in the municipalities of Grupiara and Douradoquara were randomly distributed (p = 0.27), the risk factor that proved to be statistically significant in the regression analysis for cysticercosis transmission was the water source from the river/ stream. From the analysis of the map based on the hydrography of the region, it can be observed that for the distribution of positive cases for bovine cysticercosis in the properties, the main risk factor was the interference of the rivers and their tributaries that fed the municipalities sampled. This study allowed the identification of the prevalence of the taeniasis-cysticercosis complex in the properties of the sampled municipalities (53.84% in Romaria, 45.45% in Grupiara, 37.03% in Iraí de Minas and 35.29% in Douradoquara). The results from this study will help in the prevention and control of this zoonosis in the researched areas. Key words: Bovine cysticercosis. Risk factors. Spatial distribution. Hydrography. ResumoA cisticercose acomete aos bovinos, que desenvolvem a larva da Taenia saginata, a partir da ingestão de seus ovos que são liberados pelos seres humanos. É uma parasitose que tem distribuição mundial. O uso do geoprocessamento possibilita melhor definição de áreas endêmicas e das populações sob diferentes níveis de risco, criando base para programas de controle específicos para cada sistema de criação agropecuária. O objetivo deste trabalho foi avaliar a situação epidemiológica da cisticercose bovina, em propriedades de quatro municípios da região do Triângulo Mineiro (Romaria, Iraí de Minas, Grupiara e Douradoquara), com especial atenção sobre a influência da rede hidrog...

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Evaluation of the ELISA test for the antibody detection in cattle naturally and experimentally infected with Cysticerccus bovis

Cited by 14 publications

References 9 publications

Prevalência do complexo teníase-cisticercose na zona rural de Matias Barbosa-MG

Prevalência do complexo teníase-cisticercose na zona rural de Matias Barbosa-MG

Perfil da transmissão e prevalência da cisticercose bovina em propriedades rurais do Triângulo Mineiro

Epidemiological aspects of cysticercose in relation to hydrographic net at triangulo mineiro, MG, Brazil

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