Evaluation of the entomopathogenic fungus Metarhizium brunneum and the predatory mite Stratiolaelaps scimitus against Rhizoglyphus robini under laboratory conditions

Ghalehgolabbehbahani, Arash; Sullivan, Cheryl Frank; Davari, Agrin; Parker, Bruce L.; Razavi, A.; Skinner, Margaret

doi:10.1007/s10493-022-00719-6

Cited by 3 publications

(2 citation statements)

References 36 publications

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“…Muscardine cadaver larvae of S. litura were disinfected with 75% (v/v) anhydrous ethanol for 30 s and with 1% (v/v) sodium hypochlorite for 2 min; rinsed with sterile water three times; the surface water was absorbed with a sterile filter paper; cut into five sections using a sterile scalpel; placed on Potato Dextrose Agar (PDA) medium (Sangon Biotech, Shanghai, China), which contained 1 mL ampicillin per liter at a concentration of 100 μg/mL; and incubated in a constant temperature incubator at 26°C for 5–7 days until mycelium grew. The strain was purified by the single-spore isolation method as described previously ( Ghalehgolabbehbahani et al, 2022 ), and the purified strain was designated AnS1Gz1-1.…”

Section: Methodsmentioning

confidence: 99%

First record of Aspergillus nomiae as a broad-spectrum entomopathogenic fungus that provides resistance against phytopathogens and insect pests by colonization of plants

Zhang,

Tian,

Sui

et al. 2024

Front. Microbiol.

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IntroductionAspergillus nomiae is known as a pathogenic fungus that infects humans and plants but has never been reported as an entomophagous fungus (EPF) that can provide other functions as an endotype.MethodsA strain of EPF was isolated and identified from diseased larvae of Spodoptera litura in a soybean field and designated AnS1Gzl-1. Pathogenicity of the strain toward various insect pests was evaluated, especially the ability to colonize plants and induce resistance against phytopathogens and insect pests.ResultsThe isolated EPF strain AnS1Gzl-1 was identified as A. nomiae; it showed strong pathogenicity toward five insect pests belonging to Lepidoptera and Hemiptera. Furthermore, the strain inhibited the growth of Sclerotinia sclerotiorum in vitro, a causal agent of soil-borne plant disease. It colonized plants as an endophyte via root irrigation with a high colonization rate of 90%, thereby inducing plant resistance against phytopathogen infection, and disrupting the feeding selectivity of S. litura larvae.DiscussionThis is the first record of a natural infection of A. nomiae on insects. A. nomiae has the potential to be used as a dual biocontrol EPF because of its ability to not only kill a broad spectrum of insect pests directly but also induce resistance against phytopathogens via plant colonization.

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Section: Methodsmentioning

confidence: 99%

First record of Aspergillus nomiae as a broad-spectrum entomopathogenic fungus that provides resistance against phytopathogens and insect pests by colonization of plants

Zhang,

Tian,

Sui

et al. 2024

Front. Microbiol.

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“…Isolation and culture of the EPF strain M. rileyi-killed larvae of S. litura were disinfected with 75% (v/v) anhydrous ethanol for 30 s, 1% sodium hypochlorite for 2 min, rinsed with sterile water three times, surface water was absorbed with sterile filter paper, cut into five sections used a sterile scalpel, placed on potato dextrose agar (PDA) medium (Sangon Biotech, Shanghai, China) containing 1 mL of ampicillin per liter at a concentration of 100 μg mL −1 , and incubated in a constant temperature incubator at 26 °C for 5-7 days until mycelium grew. 27 The strain was purified by the single-spore isolation method as described previously, 29 and the purified strain was designated MrS1Gz1-1.…”

Section: 21mentioning

confidence: 99%

Metarhizium rileyi with broad‐spectrum insecticidal ability confers resistance against phytopathogens and insect pests as a phytoendophyte

Zhang,

Sui,

Tian

et al. 2024

Pest Management Science

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BACKGROUNDEntomophagous fungi (EPF) not only directly kill insect pests, but also colonise plants and improve their resistance against pests. However, most previous research has focused on Beauveria bassiana and Metarhizium anisopliae, and there are few reports on whether other EPF can enhance resistance against pests via endogenous colonization. Herein, an EPF strain was isolated from diseased larvae of Spodoptera litura in a soybean field, and subjected to genome‐wide sequencing at the chromosomal level. The pathogenicity of the isolate toward various pest insects was evaluated, and the ability to colonize plants and induce resistance against phytopathogens and insect pests of were tested.RESULTSThe purified isolate was identified as M. rileyi and designated MrS1Gz1–1. Biological assays revealed its strong pathogenicity toward five insect pests belonging to Lepidoptera and Hemiptera. Furthermore, the strain inhibited the growth of soil‐borne plant disease caused by Sclerotinia sclerotiorum in vitro. It colonized plants as an endophyte via soil application, thereby inducing plant resistance‐related genes against phytopathogen infection, and it disrupted the feeding selectivity of S. litura larvae.CONCLUSIONM. rileyi MrS1Gz1–1 has potential as a broad‐spectrum microbial control agent that can induce resistance against phytopathogens and insect pests feeding as an endotype. The complete genome provides a valuable resource for exploring host interactions.

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Isolation and identification of Metarhizium guizhouense Xct1 and its pathogenicity to Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Wei

Wang

Nima

et al. 2023

Egypt J Biol Pest Control

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Background The fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), is one of the most destructive invasive pests causing serious damage to maize crops in China. At present, the use of chemical pesticides is the main method to prevent and control S. frugiperda. However, the excessive use of chemical pesticides not only causes toxicity in humans and animals but also pollutes the environment and causes public harm. In this context, the present study aimed to isolate, identify and evaluate the pathogenicity of the native strain of Metarhizium guizhouense against eggs, larvae and pupae of S. frugiperda under laboratory conditions. Results The fungal isolate was identified as M. guizhouense (Xct1) based on its morphological and molecular characteristics by sequencing ITS, β-tubulin and EF1-α gene. Meanwhile, the fungal isolate was highly pathogenic to eggs of the S. frugiperda, at the highest concentration of 1 × 108 spores/ml, the cumulative egg hatching rate was only 8.33% at 10 days post exposure. After 10 days of exposure to the highest concentration of 1 × 108 spores/ml, the LC50 values for the first–fifth larval instars of S. frugiperda were 8.12 × 104, 1.11 × 105, 5.55 × 105, 3.87 × 106 and 1.43 × 107 spores/ml, respectively. Conclusions The pathogenicity of M. guizhouense Xct1 to S. frugiperda larvae gradually decreased with increasing the developmental stages. Metarhizium guizhouense Xct1 was more pathogenic to egg and younger larvae of S. frugiperda. This provides a basis for determining the optimal period of control.

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Evaluation of the entomopathogenic fungus Metarhizium brunneum and the predatory mite Stratiolaelaps scimitus against Rhizoglyphus robini under laboratory conditions

Cited by 3 publications

References 36 publications

First record of Aspergillus nomiae as a broad-spectrum entomopathogenic fungus that provides resistance against phytopathogens and insect pests by colonization of plants

First record of Aspergillus nomiae as a broad-spectrum entomopathogenic fungus that provides resistance against phytopathogens and insect pests by colonization of plants

Metarhizium rileyi with broad‐spectrum insecticidal ability confers resistance against phytopathogens and insect pests as a phytoendophyte

Isolation and identification of Metarhizium guizhouense Xct1 and its pathogenicity to Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

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