Evaluation of the estrogenic and oxidative stress effects of the UV filter 3-benzophenone in zebrafish (Danio rerio) eleuthero-embryos

Rodrı́guez-Fuentes, Gabriela; Sandoval-Gío, Juan José; Arroyo-Silva, Anita; Noreña-Barroso, Elsa; Escalante-Herrera, Karla S.; Olvera-Espinosa, Francisco

doi:10.1016/j.ecoenv.2015.01.033

Cited by 56 publications

(23 citation statements)

References 48 publications

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“…Benzophenone-3 (oxybenzone, BP3) is commonly used as a UV-filter in cosmetics such as sunscreens, but also as a light stabilizer in plastics such as PE. In addition, BP3 has repeatedly been reported to act as an endocrine disrupting chemical interfering with reproduction and sex hormone signaling in fish and mammals (Kim and Choi, 2014;Kinnberg et al, 2015;Rodríguez-Fuentes et al, 2015). BP3 shows low degradation rates in surface waters (Kim and Choi, 2014) and-due to its lipophilicity (log Kow 4.0)-may bioaccumulate in biota.…”

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confidence: 99%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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As wide-spread pollutants in the marine environment, microplastics (MPs) have raised public concern about potential toxic effects in aquatic organisms, and, among others, MPs were suspected to act as a vector for organic pollutants to biota. The purpose of the present study was to investigate effects by three model pollutants, oxybenzone (BP3), benzo[a]pyrene (BaP), and perfluorooctane sulfonate (PFOS) adsorbed to polyethylene MPs on the basis of a standard assay, the acute fish embryo toxicity test (FET; OECD TG 236) with zebrafish (Danio rerio) supplemented by additional endpoints such as induction of ethoxyresorufin-O-deethylase (EROD) activity, modification of cyp1a gene transcription and changes in larval swimming behavior. FET assays were performed in three laboratories using slightly different husbandry and exposure conditions, which, however, were all fully compatible with the limits defined by OECD TG 236. This allowed for testing of potential changes in the FET assay due to protocol variations. The standard endpoints of the FET (acute embryotoxicity) did not reveal any acute toxicity for both virgin MPs and MPs spiked with BP3, BaP, and PFOS. With respect to sublethal endpoints, EROD activity was increased after exposure to MPs spiked with BP3 (3 h pulse) and MPs spiked with BaP (96 h continuous exposure). Cyp1a transcription was increased upon exposure to MPs spiked with BP3 or BaP. For the selected combination of MPs particles and contaminants, the basic FET proved not sensitive enough to reveal effects of (virgin and spiked) MPs. However, given that the FET can easily be supplemented by a broad variety of more subtle and sensitive endpoints, an enhanced FET protocol may provide a relevant approach with developmental stages of a vertebrate animal model, which is not protected by current EU animal welfare legislation (Directive EU 2010/63).

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mentioning

confidence: 99%

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Cormier

Batel

Cachot

et al. 2019

Front. Environ. Sci.

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“…BP-type UV filters could cause multiple estrogenic effects, developmental and reproductive toxicity as revealed by cell-based bioassay [26,33,34,43,61]. BP-3 was reported to cause a dose-dependent increase of uterine weight of immature Long-Evans rats by the activation of ERα and ERβ [22,43].…”

Section: Endocrine Disrupting Effects Of Typical Uv Filtersmentioning

confidence: 99%

Recent Advances on Endocrine Disrupting Effects of UV Filters

Wang

Pan

et al. 2016

IJERPH

144

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Ultraviolet (UV) filters are used widely in cosmetics, plastics, adhesives and other industrial products to protect human skin or products against direct exposure to deleterious UV radiation. With growing usage and mis-disposition of UV filters, they currently represent a new class of contaminants of emerging concern with increasingly reported adverse effects to humans and other organisms. Exposure to UV filters induce various endocrine disrupting effects, as revealed by increasing number of toxicological studies performed in recent years. It is necessary to compile a systematic review on the current research status on endocrine disrupting effects of UV filters toward different organisms. We therefore summarized the recent advances on the evaluation of the potential endocrine disruptors and the mechanism of toxicity for many kinds of UV filters such as benzophenones, camphor derivatives and cinnamate derivatives.

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“…Consequently, occurrence of UV filters in aquatic ecosystems and their potential effects on non-target organisms is of growing concern (Blüthgen et al, 2012;Coronado et al, 2008;Díaz-Cruz and Barceló, 2009;Fent et al, 2010;Ghazipura et al, 2017;Kinnberg et al, 2015;Kunz et al, 2006;Liu et al, 2015;Rodríguez-Fuentes et al, 2015;Tsui et al, 2014). For instance, developmental and reproductive toxicity of oxybenzone (Coronado et al, 2008;Ghazipura et al, 2017;Kinnberg et al, 2015) in fish have been attributed to an altered hormonal balance as a result of its endocrine disrupting effects (Ghazipura et al, 2017;Kinnberg et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…xenobiotics show up to three distinct modes of action, including estrogenicity, antiestrogenicity and anti-androgenicity (Blüthgen et al, 2012;Kinnberg et al, 2015;Kunz et al, 2006). Additionally, the scarce information available regarding their effects on the antioxidant defense system in fish suggests that UV filters have adverse effects beyond hormonal activity (Liu et al, 2015;Rodríguez-Fuentes et al, 2015). For instance, a growing trend in glutathione peroxidase expression was observed in zebrafish (Danio rerio) eleuthero-embryos exposed to oxybenzone, which is an indicative of oxidative stress (Rodríguez-Fuentes et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, the scarce information available regarding their effects on the antioxidant defense system in fish suggests that UV filters have adverse effects beyond hormonal activity (Liu et al, 2015;Rodríguez-Fuentes et al, 2015). For instance, a growing trend in glutathione peroxidase expression was observed in zebrafish (Danio rerio) eleuthero-embryos exposed to oxybenzone, which is an indicative of oxidative stress (Rodríguez-Fuentes et al, 2015). In the same context, Liu and co-workers also reported that benzophenone UV filters generated oxidative stress in freshwater fish Carassius auratus (Liu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Non-targeted metabolomics reveals alterations in liver and plasma of gilt-head bream exposed to oxybenzone

et al. 2018

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The extensive use of the organic UV filter oxybenzone has led to its ubiquitous occurrence in the aquatic environment, causing an ecotoxicological risk to biota. Although some studies reported adverse effects, such as reproductive toxicity, further research needs to be done in order to assess its molecular effects and mechanism of action. Therefore, in the present work, we investigated metabolic perturbations in juvenile gilt-head bream (Sparus aurata) exposed over 14 days via the water to oxybenzone (50 mg/L). The non-targeted analysis of brain, liver and plasma extracts was performed by means of UHPLC-qOrbitrap MS in positive and negative modes with both C18 and HILIC separation. Although there was no mortality or alterations in general physiological parameters during the experiment, and the metabolic profile of brain was not affected, the results of this study showed that oxybenzone could perturb both liver and plasma metabolome. The pathway enrichment suggested that different pathways in lipid metabolism (fatty acid elongation, α-linolenic acid metabolism, biosynthesis of unsaturated fatty acids and fatty acid metabolism) were significantly altered, as well as metabolites involved in phenylalanine and tyrosine metabolism. Overall, these changes are signs of possible oxidative stress and energy metabolism modification. Therefore, this research indicates that oxybenzone has adverse effects beyond the commonly studied hormonal activity, and demonstrates the sensitivity of metabolomics to assess molecular-level effects of emerging contaminants.

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Evaluation of the estrogenic and oxidative stress effects of the UV filter 3-benzophenone in zebrafish (Danio rerio) eleuthero-embryos

Cited by 56 publications

References 48 publications

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Multi-Laboratory Hazard Assessment of Contaminated Microplastic Particles by Means of Enhanced Fish Embryo Test With the Zebrafish (Danio rerio)

Recent Advances on Endocrine Disrupting Effects of UV Filters

Non-targeted metabolomics reveals alterations in liver and plasma of gilt-head bream exposed to oxybenzone

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