Evaluation of the Growth of Salmonellae in Rappaport's Broth and in Muller‐Kauffmann's Tetrathionate Broth

Vassiliadis, P.; Patéraki, E.; Papadakis, John A.; Trichopoulos, Dimitrios

doi:10.1111/j.1365-2672.1974.tb00456.x

Cited by 27 publications

(16 citation statements)

References 17 publications

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“…It must be clearly emphasized that both media studied here supported growth of minimal numbers of salmonellas contrary to the experience of Vassiliadis, Pateraki, Papadakis & Trichopoulos (1974), who used Oxoid (CM 343) Muller-Kauffmann tetrathionate. Roberts, Boag, Hall & Shipp (1975) have also found commercial Muller-Kauffmann a disappointing medium compared with Rolfe A tetrathionate (approximately 0-032 M).…”

Section: Discussioncontrasting

confidence: 77%

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Harvey

Price

1976

J. Hyg.

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SUMMARYSelenite F broth and a modified Muller-Kauffmann tetrathionate broth were investigated using sewage-polluted natural water as inocula. The modification of the tetrathionate medium was necessary as commercial alternatives would not allow multiplication of small numbers of salmonellas.The wide range of molar concentrations in different tetrathionate broths was emphasized.Selenite F broth was more efficient than Muller-Kauffmann tetrathionate broth, in our hands, with the material tested. Direct inoculation of the enrichment media was used.Each of the two media examined had a bias towards selection of certain serotypes. If possible, both enrichment broths should be used to obtain maximum information.

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Section: Discussioncontrasting

confidence: 77%

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Harvey

Price

1976

J. Hyg.

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“…This is not unexpected as there are many reports of the toxicity of this medium to Salmonella (e.g. Vassiliadis et al 1974;van Schothorst et al 1977).…”

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confidence: 56%

Rapid and definitive detection of Salmonella in foods by PCR

Bennett

Greenwood

Tennant

et al. 1998

Letters in Applied Microbiology

105

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TMsystem for screening Salmonella is one of the first commercial PCR-based systems for the detection of food-borne pathogens. It is able to give a confirmed result within 28 h. There was 98·6% and 95·8% agreement between the BAX TM system and conventional cultural analysis for the detection of Salmonella in artificially inoculated and uninoculated food samples, respectively. In both cases, the BAX TM system generated more positive detections than cultural analysis. The speed of assay, ease of use and high specificity and sensitivity of the BAX TM system for the detection of food-borne Salmonella make it an attractive method for routine food microbiology laboratories.

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“…This difference in the inocula is inherent to the two enrichment broths, as it was observed earlier (Rappaport et al 1956;McCoy, 1962;Harvey, Price & Hall, 1973;Vassiliadis et al 1974;Van Schothorst et al 1977).…”

Section: Isolation Of Salmonella Methodsmentioning

confidence: 82%

Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium

Vassiliadis

Trichopoulos

Kalapothaki

et al. 1981

J. Hyg.

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SUMMARYOne hundred and eighty samples of pork sausages were examined after preenrichment in buffered peptone water (P medium), for the presence of salmonellas. From each pre-enrichment four enrichments were made: (1) 0-1 ml of P medium was inoculated into 10 ml of Rappaport's medium, formula R 10 (R 10/43°C), (2) 1 ml of the P medium was added to 100 ml of R 10 broth (RIO/100 ml/43°C), (3) 1 ml of P medium was inoculated into 10 ml of Muller-Kauffmann tetrathionate broth (MK medium) prepared in accordance with the International Standards Organization document ISO 3565 (MK/43°C) and (4) 10 ml of P medium were added to 100 ml of MK broth (MK/100 ml/43°C). All the enrichments were incubated at 43°C for 48 h. Forty-six and 47 samples were found positive with the first two enrichment methods (R 10/43°C and R 10/100 ml/43°C), while only 16 samples were found positive with the method MK/43°C, and 27 with the method MK/ 100 ml/43 'C. The superiority ofeither one of the two R 10 procedures over either one of the two MK methods is statistically highly significant (paired x2; P < 0-001 in all four comparisons). The superiority of procedure MK/100 ml/43 'C over the method MK/43 'C is also statistically significant (P < 0005). Both R 10/43°C and R 10/100 ml/43°C procedures had a much stronger inhibitory effect on the competing organisms (lactose-and sucrosenegative) than the two MK methods.

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Evaluation of the Growth of Salmonellae in Rappaport's Broth and in Muller‐Kauffmann's Tetrathionate Broth

Cited by 27 publications

References 17 publications

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Isolation of salmonellas from sewage-polluted river water using selenite F and Muller–Kauffmann tetrathionate

Rapid and definitive detection of Salmonella in foods by PCR

Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium

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