Evaluation of the IDI-MRSA assay on the SmartCycler real-time PCR platform for rapid detection of MRSA from screening specimens

Rapid Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) in Diverse Clinical Specimens by the BD GeneOhm MRSA Assay and Comparison with Culture

“…This may be explained by the use of enrichment broth as the reference method, which yields conceivably a higher sensitivity than does direct plating (17,20,23). Overall sensitivity of PCR for all swabs (n ϭ 1,595) in Amies agar corresponds well to the results reported previously using Stuart's medium (sensitivity ranging from 81% to 92.3%) (3,8,19,22,26). However, a direct comparison of Amies agar with Stuart's medium for the same clinical specimens has not been undertaken yet.…”

BD GeneOhm-MRSA assay for detection of methicillin-resistantStaphylococcus aureusdirectly in nasal and non-nasal Truro specimens from haematologic patients

“…The reason for the false-negative BD GeneOhm-MRSA results may be the variability in either the SCCmec or orfX sequences that did not allow amplification with the panel of primers in the assay [20]. However, pure colonies from all MRSA detected by culture were directly tested by the BD GeneOhm-MRSA assay and all were identified as MRSA.…”

Reliability of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) Assay in Detecting MRSA Isolates with a Variety of Genotypes from the United States and Taiwan

“…A more diverse collection would be difficult to find since the genotypic distribution of isolates that are naturally circulating in the United States is limited to primarily SCCmec IV and SCCmec II in ST8 and ST5 genetic backgrounds, respectively (7,25). In fact, compared with similar studies that tested the performance of the BD GeneOhm MRSA assay on MRSA isolates (1,11,20,24), our study is the largest performed to date and the only study performed in the United States.…”

“…Moreover, increasing numbers of reports have documented DNA sequence variation among SCCmec elements (1-3, 5, 9, 14, 22) (20) have reported on isolates in their collections from Denmark, Finland, Switzerland, and Ireland, respectively, that were not detected by this assay, despite the presence of an SCCmec element. We therefore sought to assess the reliability of the BD GeneOhm MRSA assay in detecting MRSA isolates that had a variety of SCCmec types and genetic backgrounds that we obtained from the United States.…”

“…The BD GeneOhm MRSA assay (BD Diagnostics GeneOhm, Québec City, Québec, Canada), approved for use for detection of MRSA colonization from nasal swabs, has been used by the University of Chicago Medical Center (UCMC) to identify patients who are colonized with MRSA upon admission to the ICU. However, since the initial validation of the assay (13), few studies have been done to validate the assay against genetically diverse MRSA isolates (1,11,20,24). To date, no study has validated the BD GeneOhm MRSA assay with MRSA isolates circulating in the United States.…”