Evaluation of the immunogenicity and protective efficacy of a candidate parainfluenza virus type 3 subunit vaccine in cotton rats

Protection of cotton rats against human parainfluenza virus type 3 by vaccination with a chimeric FHN subunit glycoprotein

“…Although none of the mixtures of F and HN proteins were completely protective, vaccination with each of the mixtures resulted in a significant reduction in the amount of PIV-3 recovered from lung tissue. Others (Ambrose et al, 1991 ;Hall et al, 1991) have shown that mixtures of the F and HN glycoproteins were also not completely protective against experimental PIV-3 infection in cotton rats. Our data suggest that simply exposing the immune system to mixtures of both the F and HN glycoproteins was no more effective than vaccination with HN protein alone.…”

“…Vaccination with either purified F or HN glycoproteins prepared from either recombinant expression vectors (Spriggs et al, 1987;Coelingh et aI., 1989;Ray et al, 1989) or by purification from infected cells has only partially protected animals from PIV-3 challenge (Ambrose et al, 1991 ;Ray et al, 1988;Hall et al, 1991). Development of an experimental subunit vaccine for complete prevention of PIV-3 replication is warranted.…”

Parainfluenza Viruses

“…The envelope glycoproteins (HN and F) of HPIV-3 have been cleaved from whole virus to create subunit vaccines that have demonstrated efficacy in rats, lambs, mice, and hamsters (4,246,300,303). Many different routes of immunization have been tried, including subcutaneous, intramuscular, and intranasal.…”

Comparative analysis of the immunoprotective abilities of glycosylated and deglycosylated parainfluenza virus type 3 surface glycoproteins

“…Fully glycosylated, affinity-purified PIV-3 HN and F proteins elicited a protective response in cotton rats and hamsters (Ambrose et al, 1991 ;Ewasyshyn et al, 1992), whereas the immunoprotective ability of the deglycosylated antigens has not yet been reported. Thus, the objective of this study was to determine the role of the carbohydrate moieties in the immunoprotective ability of the PIV-3 HN and F proteins.…”

“…There was no significant difference between the lung virus titres of animals immunized with two 0.1 lag doses of either preparation, and titres were significantly lower than for control animals. It has been reported previously that the titre of infectious virus present in the lungs of hamsters (Ewasyshyn et al, 1992) and cotton rats (Ambrose et al, 1991) immunized with the glycosylated HN and F proteins correlated inversely with serum HAI and neutralizing antibody titres. Although serum neutralizing titres in the 8 week sample were significantly lower in animals immunized with 1.0 lag of deglycosylated proteins, antibody levels were still within the protective range.…”