Evaluation of the Immunogenicity in Mice Orally Immunized with Recombinant Lactobacillus casei Expressing Porcine Epidemic Diarrhea Virus S1 Protein

Xiao, Yao; Wang, Xiaona; Li, Yue; Li, Fengsai; Zhao, Haiyuan; Shao, Yilan; Zhang, Liu; Ding, Guojie; Li, Jiaxuan; Jiang, Yanping; Cui, Wen; Shan, Zhifu; Zhou, Han; Wang, Li; Qiao, Xinyuan; Tang, Lijie; Li, Yijing

doi:10.3390/v14050890

Cited by 8 publications

(5 citation statements)

References 44 publications

(46 reference statements)

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“…Another in vivo study demonstrated induction of innate and adaptive immune responses by L. acidophilus NCFB 1748 and L. paracasei DC412 on BALB/c inbred mice and Fisher-344 inbred rats through polymorphonuclear (PMN) cell recruitment, TNF-α secretion and phagocytosis ( Azad et al, 2018 ). Lactobacilli encode for certain motifs within their genome, such as unmethylated CpG motifs which are recognized by TLRs that are expressed particularly in B cells and macrophages ( Xiao et al, 2022 ). The role of Lactobacillus spp.…”

Section: Genus Lactobacillus and Human Healthmentioning

confidence: 99%

Gut microbiome and human health: Exploring how the probiotic genus Lactobacillus modulate immune responses

2022

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The highest density of microbes resides in human gastrointestinal tract, known as “Gut microbiome”. Of note, the members of the genus Lactobacillus that belong to phyla Firmicutes are the most important probiotic bacteria of the gut microbiome. These gut-residing Lactobacillus species not only communicate with each other but also with the gut epithelial lining to balance the gut barrier integrity, mucosal barrier defence and ameliorate the host immune responses. The human body suffers from several inflammatory diseases affecting the gut, lungs, heart, bone or neural tissues. Mounting evidence supports the significant role of Lactobacillus spp. and their components (such as metabolites, peptidoglycans, and/or surface proteins) in modulatingimmune responses, primarily through exchange of immunological signals between gastrointestinal tract and distant organs. This bidirectional crosstalk which is mediated by Lactobacillus spp. promotes anti-inflammatory response, thereby supporting the improvement of symptoms pertaining to asthma, chronic obstructive pulmonary disease (COPD), neuroinflammatory diseases (such as multiple sclerosis, alzheimer’s disease, parkinson’s disease), cardiovascular diseases, inflammatory bowel disease (IBD) and chronic infections in patients. The metabolic disorders, obesity and diabetes are characterized by a low-grade inflammation. Genus Lactobacillus alleviates metabolic disorders by regulating the oxidative stress response and inflammatory pathways. Osteoporosis is also associated with bone inflammation and resorption. The Lactobacillus spp. and their metabolites act as powerful immune cell controllers and exhibit a regulatory role in bone resorption and formation, supporting bone health. Thus, this review demonstrated the mechanisms and summarized the evidence of the benefit of Lactobacillus spp. in alleviating inflammatory diseases pertaining to different organs from animal and clinical trials. The present narrative review explores in detail the complex interactions between the gut-dwelling Lactobacillus spp. and the immune components in distant organs to promote host’s health.

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Section: Genus Lactobacillus and Human Healthmentioning

confidence: 99%

Gut microbiome and human health: Exploring how the probiotic genus Lactobacillus modulate immune responses

2022

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“…Xiao et al utilized the strain L. casei deficient in the upp gene to express the PEDV S1 protein, orally immunized mice, and measured cytokine levels. The data revealed the successful induction of Th1, Th2, and Th17 cell immune responses (Xiao et al 2022 ). Zhang et al developed the alanine racemase-deficient L. casei complementary system to express the VP4 gene of porcine rotavirus.…”

Section: Discussionmentioning

confidence: 97%

“…The plasmid pLCNICK (Song et al 2017 ), including the thermosensitive replicon, NCas 9 protein, and sgRNA sequence, was generously provided by Yang Sheng (a researcher at the Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences). Plasmids pMD-19Ts-P776 (Yin et al 2016 ), pMD-19Ts-RBS, and pMD-19Ts-S1 (Xiao et al 2022 ) were preserved in our laboratory. The PEDV LJB2019 strain was isolated from clinical samples and stored at − 140 °C to maintain its virulence.…”

Section: Methodsmentioning

confidence: 99%

“…The precipitate was incubated with 1% lysozyme and sonicated for 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) detection and western blotting. SDS-PAGE was used to separate the proteins in the mixture, which were then transferred onto PVDF membranes (Millipore, Milford, MA, USA) using a mouse anti-S1 monoclonal antibody (mAb cell supernatant) prepared in our laboratory as the primary antibody (Xiao et al 2022 ). Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody (Sigma, Ronkonkoma, NY, USA) was used as the secondary antibody at a dilution of 1:5000.…”

Section: Methodsmentioning

confidence: 99%

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Assessing immunogenicity of CRISPR-NCas9 engineered strain against porcine epidemic diarrhea virus

Li,

Zhao,

Sui

et al. 2024

Appl Microbiol Biotechnol

Self Cite

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Porcine epidemic diarrhea (PED) caused by porcine epidemic diarrhea virus (PEDV), is an acute and highly infectious disease, resulting in substantial economic losses in the pig industry. Given that PEDV primarily infects the mucosal surfaces of the intestinal tract, it is crucial to improve the mucosal immunity to prevent viral invasion. Lactic acid bacteria (LAB) oral vaccines offer unique advantages and potential applications in combatting mucosal infectious diseases, making them an ideal approach for controlling PED outbreaks. However, traditional LAB oral vaccines use plasmids for exogenous protein expression and antibiotic genes as selection markers. Antibiotic genes can be diffused through transposition, transfer, or homologous recombination, resulting in the generation of drug-resistant strains. To overcome these issues, genome-editing technology has been developed to achieve gene expression in LAB genomes. In this study, we used the CRISPR-NCas9 system to integrate the PEDV S1 gene into the genome of alanine racemase-deficient Lactobacillus paracasei △Alr HLJ-27 (L. paracasei △Alr HLJ-27) at the thymidylate synthase (thyA) site, generating a strain, S1/△Alr HLJ-27. We conducted immunization assays in mice and piglets to evaluate the level of immune response and evaluated its protective effect against PEDV through challenge tests in piglets. Oral administration of the strain S1/△Alr HLJ-27 in mice and piglets elicited mucosal, humoral, and cellular immune responses. The strain also exhibited a certain level of resistance against PEDV infection in piglets. These results demonstrate the potential of S1/△Alr HLJ-27 as an oral vaccine candidate for PEDV control. Key points • A strain S1/△Alr HLJ-27 was constructed as the candidate for an oral vaccine. • Immunogenicity response and challenge test was carried out to analyze the ability of the strain. • The strain S1/△Alr HLJ-27 could provide protection for piglets to a certain extent.

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“…To date, researchers have expressed partial S protein, including the collagenase equivalent domain (COE), receptor binding domain (RBD) and S1, or the full-length S protein of individual SECs using E. coli , Lactobacillus , Bacillus subtilis , yeast, 293T cells, virus expression system (e.g., vesicular stomatitis virus, swinepox virus, pseudorabies virus, baculovirus, and human adenovirus), or transgenic plant expression systems ( Table 3 ). Among them, the immunogenicity of 14 vaccine candidates was evaluated in mice [ 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 ]. All these vaccine candidates induced high levels of the virus-specific IgG and VN antibodies in serum and cytokines in splenocytes.…”

Section: Sec Vaccine Development In Chinamentioning

confidence: 99%

Prevention and Control of Swine Enteric Coronaviruses in China: A Review of Vaccine Development and Application

Kong,

Jia,

Xiao

et al. 2023

Vaccines

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Swine enteric coronaviruses (SECs) cause significant economic losses to the pig industry in China. Although many commercialized vaccines against transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are available, viruses are still widespread. The recent emergence of porcine deltacoronavirus (PDCoV) and swine acute diarrhea syndrome coronavirus (SADS-CoV), for which no vaccines are available, increases the disease burden. In this review, we first introduced the genomic organization and epidemiology of SECs in China. Then, we discussed the current vaccine development and application in China, aiming to provide suggestions for better prevention and control of SECs in China and other countries.

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Evaluation of the Immunogenicity in Mice Orally Immunized with Recombinant Lactobacillus casei Expressing Porcine Epidemic Diarrhea Virus S1 Protein

Cited by 8 publications

References 44 publications

Gut microbiome and human health: Exploring how the probiotic genus Lactobacillus modulate immune responses

Gut microbiome and human health: Exploring how the probiotic genus Lactobacillus modulate immune responses

Assessing immunogenicity of CRISPR-NCas9 engineered strain against porcine epidemic diarrhea virus

Prevention and Control of Swine Enteric Coronaviruses in China: A Review of Vaccine Development and Application

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