Evaluation of the Impact of Methicillin‐Resistant <i>Staphylococcus pseudintermedius</i> Biofilm Formation on Antimicrobial Susceptibility

Walker, Meagan; Singh, Ameet; Nazarali, Alim; Gibson, Thomas W.G.; Rousseau, Joyce D.; Weese, J. Scott

doi:10.1111/vsu.12535

Cited by 15 publications

(12 citation statements)

References 18 publications

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“…This positioning, combined with the dog's incontinence, almost certainly played a role in the development of urinary tract infection within a month of surgery. While resolution of urinary tract infection has been reported after subcutaneous ureteral bypass in cats, biofilm formation can make clearance challenging . Even if infection could be cleared, the positioning of implants for the dog in this study would have predisposed to reinfection.…”

Section: Discussionmentioning

confidence: 91%

Interventions and experience after complicated total cystectomy in a dog with transitional cell carcinoma

2019

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Objective: To report surgical and postoperative management of complicated total cystectomy in a dog with transitional cell carcinoma (TCC). Study design: Case report. Animals: One male neutered Shetland sheepdog.Methods: The dog was presented after a 1-month history of stranguria, unresponsive to oral antibiotic therapy. A craniodorsal bladder mass was identified by computed tomography (CT), and partial cystectomy was performed with 1-cm gross lateral margins (day 1). Results of histopathology provided evidence for a diagnosis of TCC, and the dog was treated with adjuvant mitoxantrone. The dog presented with uroperitoneum on day 67 after recurrence and spontaneous perforation. Total cystectomy and ureterourethral anastomosis were performed. Ureteral obstruction developed after removal of catheters that had been placed intraoperatively. Surgical revision included resection of the anastomosis site, bilateral ureteral stenting, and transection and reorientation of the distal urethra to facilitate tension-free closure. Postoperative leakage was managed with bilateral percutaneous nephrostomy tube placement. Results: The dog was discharged on day 88. Adjuvant treatment with vinblastine was pursued. Local recurrence was noted at day 154. Subcutaneous ureteral bypass was performed on day 247 to manage repeat obstruction. Repeated urinary tract infections were subsequently encountered. The dog was euthanized on day 368 because of abdominal discomfort and inappetence, with evidence of progressive urethral, ureteric, and abdominal wall TCC. Conclusion: Complicated cystectomy can be managed to provide survival comparable to previous reports regarding total cystectomy. Clinical significance: Nephrostomy tube placement, ureteral stenting, and subcutaneous ureteral bypass may be considered to manage complicated cystectomy.Preemptive stenting or urinary diversion may help prevent complications.

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Section: Discussionmentioning

confidence: 91%

Interventions and experience after complicated total cystectomy in a dog with transitional cell carcinoma

2019

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“…Further studies are needed to clarify the penetration and killing mechanisms. However, these findings might be of therapeutic interest because biofilm significantly affects antimicrobial susceptibility of both MSSP and MRSP from dogs [7]. Of interest, human S. pseudintermedius isolates also form structurally complex biofilm that are intrinsically resistant to antibiotics [34], thus complicating zoonotic infections.…”

Section: Discussionmentioning

confidence: 99%

“…Biofilm formation can facilitate the colonization of different body sites in dogs and hinder the infection treatment. Walker et al reported MIC values for biofilm-associated S. pseudintermedius significantly higher than for planktonic bacteria [7]. In another study, Meroni et al reported that 68% of S. pseudintermedius isolates were able to form biofilms, but 100% of the multidrug resistant strains were slime producers; therefore, it seems that biofilm production can facilitate bacteria horizontal gene transfer [8].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius

et al. 2021

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Resin acids are valued in traditional medicine for their antiseptic properties. Among these, abietic acid has been reported to be active against methicillin-resistant Staphylococcus aureus (MRSA) strains. In veterinary healthcare, the methicillin-resistant Staphylococcus pseudintermedius (MRSP) strain is an important reservoir of antibiotic resistance genes including mecA. The incidence of MRSP has been increasing, and treatment options in veterinary medicine are partial. Here, we investigated the antimicrobial and antibiofilm properties of abietic acid against three MRSP and two methicillin-susceptible Staphylococcus pseudintermedius (MSSP) strains, isolated from diseased pet animals and human wound samples. Abietic acid showed a significant minimal inhibitory concentration (MIC) value ranging from 32 to 64 μg/mL (MRSPs) and 8 μg/mL (MSSP). By checkerboard method we demonstrated that abietic acid increased oxacillin susceptibility of MRSP strains, thus showing a synergistic interaction with oxacillin. Abietic acid was also able to contrast the vitality of treated MSSP and MRSP1 biofilms at 20 μg/mL and 40 μg/mL, respectively. Finally, the compound moderately reduced mecA, mecR1 and mec1 gene expression. In conclusion, the results here reported demonstrate the antimicrobial activity of abietic acid against MRSP and support the use of this compound as a potential therapeutic agent to be used in combinatorial antibiotic therapy.

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“…While concern over resistance to topically used antibacterial agents exists, clinical treatment failure of topical anti-staphylococcal therapy has not been conclusively reported, to the authors' knowledge; MICs for staphylococci from animals have been consistently low and are likely to be substantially exceeded by achievable topical drug concentrations (Loeffler et al 2008;Valentine et al, 2012;Clark et al, 2015). However, continual monitoring of resistance and clinical efficacy, further evaluation of alternatives such as hypochlorite (bleach), Manuka honey, of potentially synergistic combinations and of anti-biofilm products will be critical (Walker et al, 2016).…”

Section: Topical Therapymentioning

confidence: 99%

What has changed in canine pyoderma? A narrative review

Loeffler

Lloyd

2018

The Veterinary Journal

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Evaluation of the Impact of Methicillin‐Resistant Staphylococcus pseudintermedius Biofilm Formation on Antimicrobial Susceptibility

Cited by 15 publications

References 18 publications

Interventions and experience after complicated total cystectomy in a dog with transitional cell carcinoma

Interventions and experience after complicated total cystectomy in a dog with transitional cell carcinoma

Synergistic Effect of Abietic Acid with Oxacillin against Methicillin-Resistant Staphylococcus pseudintermedius

What has changed in canine pyoderma? A narrative review

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