Evaluation of the in vitro activity of tigecycline against multiresistant Gram-positive cocci containing tetracycline resistance determinants

Borbone, Sonia; Lupo, Agnese; Mezzatesta, Maria Lina; Campanile, Floriana; Santagati, Maria; Stefani, Stefania

doi:10.1016/j.ijantimicag.2007.03.014

Cited by 15 publications

(12 citation statements)

References 26 publications

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“…The results of this study slightly differed from other data on European and North American antibiotic-resistant clinical isolates that were phenotypically characterized [13–15]. In a recent study, the in vitro activity of tigecycline against 38 MRSA and the correlation of this activity with their resistance gene content were determined [16]. Tigecycline demonstrated good activity against MRSA, with MIC 50 and MIC 90 values of 0.12 and 0.25 μg/ml, respectively.…”

Section: Resultsmentioning

confidence: 61%

In vitro activity of tigecycline against methicillin-resistant Staphylococcus aureus, including livestock-associated strains

Verkade

Verhulst

Huijsdens

et al. 2010

Eur J Clin Microbiol Infect Dis

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The in vitro activity of tigecycline was determined using a well-defined collection of methicillin-resistant Staphylococcus aureus (MRSA) isolates (n = 202), including 33 livestock-associated strains. Susceptibility testing was performed using the Etest system. Among the 202 MRSA strains, three (1.5%) had a minimum inhibitory concentration (MIC) value for tigecycline greater than 0.5 mg/l, which are considered to be resistant. When these strains were tested using Iso-Sensitest medium, the MICs were substantially lower and no resistance was found. This discrepancy warrants further investigations into the preferred test conditions for tigecycline. In conclusion, tigecycline showed good activity against MRSA strains in vitro.

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Section: Resultsmentioning

confidence: 61%

In vitro activity of tigecycline against methicillin-resistant Staphylococcus aureus, including livestock-associated strains

Verkade

Verhulst

Huijsdens

et al. 2010

Eur J Clin Microbiol Infect Dis

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“…Susceptibility to erythromycin, azithromycin, clindamycin and tetracycline was performed by the broth dilution method, as recommended by the Clinical and Laboratory Standards Institute (Standard, 2006). The resistance genes – erm, mef , and tet – were studied by PCR using previously published primers and protocols, followed by sequencing (Borbone et al , 2007).…”

Section: Methodsmentioning

confidence: 99%

Conjugal mobilization of the mega element carryingmef(E) fromStreptococcus salivariustoStreptococcus pneumoniae

Santagati¹,

Lupo²,

Scillato³

et al. 2009

FEMS Microbiology Letters

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We report the isolation and characterization of an unusual strain of Streptococcus salivarius, 3C30, displaying both the macrolide-lincosamide-streptogramin B and the tetracycline resistance phenotypes. It harbours the mef(E), erm(B), and tet(M) genes carried by different genetic elements. The genetic element carrying mef(E), named mega, was investigated by long PCR and sequencing, while the presence of the Tn3872-like element, carrying tet(M) and erm(B), was demonstrated by sequencing of both the int-xis-Tn and the fragment between the two resistance genes. In strain 3C30 the mega element is 5388 bp in size and its nucleotide sequence is identical to that of the element described previously in S. salivarius, with the exception of a 912 bp deletion at the left end. The composite Tn3872-like element appeared to be nonconjugative while the mega element was transferred by conjugation to Streptococcus pneumoniae. It was, however, impossible to transfer it again from these transconjugants to other strains. In addition, only in the 3C30 strain did mega form circular structures, as identified by real-time PCR. In conclusion, we found a clinical strain of S. salivarius carrying both mega and Tn3872-like genetic elements. Mega is transferable by conjugation to S. pneumoniae but it is not transferable again from the transconjugants, suggesting a possible mobilization by recombinases of the coresident Tn3872-like transposon.

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“…Tigecycline has potent in vitro activity against a wide range of Gram‐positive and Gram‐negative bacteria, including MRSA and methicillin‐resistant Staphylococcus epidermidis [259–268].…”

Section: Tigecyclinementioning

confidence: 99%

New therapeutic choices for infections caused by methicillin-resistant Staphylococcus aureus

Bouza

2009

Clinical Microbiology and Infection

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Evaluation of the in vitro activity of tigecycline against multiresistant Gram-positive cocci containing tetracycline resistance determinants

Cited by 15 publications

References 26 publications

In vitro activity of tigecycline against methicillin-resistant Staphylococcus aureus, including livestock-associated strains

In vitro activity of tigecycline against methicillin-resistant Staphylococcus aureus, including livestock-associated strains

Conjugal mobilization of the mega element carryingmef(E) fromStreptococcus salivariustoStreptococcus pneumoniae

New therapeutic choices for infections caused by methicillin-resistant Staphylococcus aureus

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