Evaluation of the In Vitro Stability of Stimuli-Sensitive Fatty Acid-Based Microparticles for the Treatment of Lung Cancer

Abstract

The fatty acid-based microparticles containing iron oxide nanoparticles and paclitaxel (PAX) are a viable proposition for the treatment of lung cancer. The microparticles inhaled as a dry powder can be guided to selected locations using an external magnetic field, and when accumulated there, the active compound release can be triggered by local hyperthermia. However, this general strategy requires that the active compound is released from microparticles and can reach the targeted cells before microparticles are removed. Isothermal titration calorimetry was used to demonstrate that the components of microparticles were released and transferred to albumins and lipid bilayers. The morphology of the measured particulates was studied with scanning electron microscopy and dynamic light scattering. To determine the cytotoxicity of microparticles, cell culture studies were done. It has been shown that the transfer efficiency depends predominantly on the fatty acid composition of microparticles, which, together with the active ingredient, accumulate predominantly in membrane structures after being released from microparticles and before entering the cytoplasm. The release process is sufficient; hence, paclitaxel-loaded microparticles effectively suppressed the proliferation of A549 human lung epithelial cells of malignant origin (IC 50 values for both lauric acid-based and myristic/palmitic-based microparticles containing paclitaxel were below 0.375 μg/mL), while reference microparticles were noncytotoxic.

Improved Magneto-Microfluidic Separation of Nanoparticles through Formation of the β-Cyclodextrin–Curcumin Inclusion Complex

“…For many decades, magnetic nanoparticles have attracted the attention of researchers and various manufacturers in regard to their biomedical − and environmental applications. − In many of these applications, one needs to manipulate the nanoparticles by gradient magnetic fields, as in the case of magnetic drug delivery, i.e., guiding and controlled release of a drug from the nanoparticle surface to a localized treatment area. − The migration of magnetic nanoparticles along the magnetic field gradient is referred to as magnetophoresis, and its intensity is characterized by the magnetophoretic mobility, defined as a proportionality factor between the particle velocity and the magnetic field gradient. In other applications, one needs to separate magnetic nanoparticles from the solvent capturing them on magnetized collectors.…”