Evaluation of the infiltration of polystyrene nanobeads in zebrafish embryo tissues after short-term exposure and the related biochemical and behavioural effects

Parenti, Camilla Carla; Ghilardi, Anna; Torre, Camilla Della; Magni, Stefano; Giacco, Luca Del; Binelli, Andrea

doi:10.1016/j.envpol.2019.07.115

Cited by 89 publications

(35 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, any behavioural variation caused by the exposure to environmental contaminants may compromise directly or indirectly the organism survival and fitness. Behavioural effects caused by MPs and NPs, in terms of changes in locomotor activity and feeding behaviour, were already observed in several aquatic organisms, such as Daphnia magna (De Felice et al, 2019), Carcinus maenas (Watts et al, 2014) and Danio rerio embryos and adults (Parenti et al, 2019;Qiang and Cheng, 2019;Mak et al, 2019).…”

Section: Behavioural Testsmentioning

confidence: 81%

“…The measurement of GST, SOD and CAT, as well as the ROS production followed the methods described by Parenti et al (2019), with minor modifications. The day of the analyses, homogenates were thawed, and protein concentration was measured by the Coomassie Brilliant Blue G-250 (Pierce) protein assay, with bovine serum albumin as standard.…”

Section: Biomarkers Of Oxidative Stressmentioning

confidence: 99%

See 1 more Smart Citation

Ingestion and effects of polystyrene nanoparticles in the silkworm Bombyx mori

et al. 2020

View full text Add to dashboard Cite

This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

show abstract

Section: Behavioural Testsmentioning

confidence: 81%

Section: Biomarkers Of Oxidative Stressmentioning

confidence: 99%

Ingestion and effects of polystyrene nanoparticles in the silkworm Bombyx mori

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The navigation strategy was measured as turn angle used by the animal to change the moving direction of the body’s central point [ 33 , 34 , 35 ]. In untreated controls, the turn angle varied within the light–dark cycle: during the light period, turn angle increased (for instance, the turn angle at 4 h was 76 ± 3°), while in the dark period it decreased (64 ± 7°, Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

Tuning the Reduction of Graphene Oxide Nanoflakes Differently Affects Neuronal Networks in the Zebrafish

et al. 2021

View full text Add to dashboard Cite

The increasing engineering of biomedical devices and the design of drug-delivery platforms enriched by graphene-based components demand careful investigations of the impact of graphene-related materials (GRMs) on the nervous system. In addition, the enhanced diffusion of GRM-based products and technologies that might favor the dispersion in the environment of GRMs nanoparticles urgently requires the potential neurotoxicity of these compounds to be addressed. One of the challenges in providing definite evidence supporting the harmful or safe use of GRMs is addressing the variety of this family of materials, with GRMs differing for size and chemistry. Such a diversity impairs reaching a unique and predictive picture of the effects of GRMs on the nervous system. Here, by exploiting the thermal reduction of graphene oxide nanoflakes (GO) to generate materials with different oxygen/carbon ratios, we used a high-throughput analysis of early-stage zebrafish locomotor behavior to investigate if modifications of a specific GRM chemical property influenced how these nanomaterials affect vertebrate sensory-motor neurophysiology—exposing zebrafish to GO downregulated their swimming performance. Conversely, reduced GO (rGO) treatments boosted locomotor activity. We concluded that the tuning of single GRM chemical properties is sufficient to produce differential effects on nervous system physiology, likely interfering with different signaling pathways.

show abstract

“…Successful treated embryos were incubated at room temperature (25-28 • C) for 5 days. The cumulative mortality and developmental malformations of embryos and larvae were observed and determined every 24 h from 0-120 HPF as previously suggested by Parenti et al, [56]. The results indicate that mortality, hatching rate, heart rate, morphological malformation or teratogenic defects were observed using THUNDER Imager 3D Live Cell & 3D Cell Culture & 3D Assay, Leica Microsystems GmbH, Wetzlar, Germany.…”

Section: Zebrafish Embryo Toxicity (Zfet) Assaymentioning

confidence: 89%

Use of Zebrafish Embryo Assay to Evaluate Toxicity and Safety of Bioreactor-Grown Exopolysaccharides and Endopolysaccharides from European Ganoderma applanatum Mycelium for Future Aquaculture Applications

Wan‐Mohtar

Ilham

Jamaludin

et al. 2021

IJMS

View full text Add to dashboard Cite

Natural mycelial exopolysaccharide (EPS) and endopolysaccharide (ENS) extracted from bioreactor-cultivated European Ganoderma applanatum mushrooms are of potential high commercial value for both food and adjacent biopharmaceutical industries. In order to evaluate their potential toxicity for aquaculture application, both EPS (0.01–10 mg/mL) and ENS (0.01–10 mg/mL) extracts were tested for Zebrafish Embryo Toxicity (ZFET); early development effects on Zebrafish Embryos (ZE) were also analyzed between 24 and 120 h post-fertilization (HPF). Both EPS and ENS are considered non-toxic with LC50 of 1.41 mg/mL and 0.87 mg/mL respectively. Both EPS and ENS did not delay hatching and teratogenic defect towards ZE with <1.0 mg/mL, respectively. No significant changes in the ZE heart rate were detected following treatment with the two compounds tested (EPS: 0.01–10 mg/mL: 176.44 ± 0.77 beats/min and ENS: 0.01–10 mg/mL: 148.44 ± 17.75 beats/min) compared to normal ZE (120–180 beats/min). These initial findings support future pre-clinical trials in adult fish models with view to safely using EPS and ENS as potential feed supplements for supplements for development of the aquaculture industry.

show abstract

Evaluation of the infiltration of polystyrene nanobeads in zebrafish embryo tissues after short-term exposure and the related biochemical and behavioural effects

Cited by 89 publications

References 57 publications

Ingestion and effects of polystyrene nanoparticles in the silkworm Bombyx mori

Ingestion and effects of polystyrene nanoparticles in the silkworm Bombyx mori

Tuning the Reduction of Graphene Oxide Nanoflakes Differently Affects Neuronal Networks in the Zebrafish

Use of Zebrafish Embryo Assay to Evaluate Toxicity and Safety of Bioreactor-Grown Exopolysaccharides and Endopolysaccharides from European Ganoderma applanatum Mycelium for Future Aquaculture Applications

Contact Info

Product

Resources

About