Evaluation of the Microbiological Effect of Colloidal Nanosilver Solution for Root Canal Treatment

Abstract

Background: The goal of endodontic treatment, along with the preparation of the root canal and giving it a shape corresponding to the obturation technique, is the drug treatment of the canal. The aim of this study was to determine the antibacterial effect of a colloidal solution of nanosilver at its various dilutions on root canal microorganism. Materials and methods: A solution of silver nanoparticles at a concentration of 10,000 ppm (1.0%) was diluted in various concentrations (10 solutions from 1% to 0.0025%). Cultures used for research: Str. agalacticae ATCC 3984, E. faecalis ATCC 323, St. aureus ATCC 4785, C. albicans ATCC 10231. After thawing, cultures of microorganisms were introduced into a liquid nutrient medium: cerebral heart broth for bacterial cultures and Sabouraud broth for C. albicans. The cultivation was carried out at a temperature of 37 °C for 24 h. A bacterial suspension for inoculation was prepared from a microbial sediment according to a turbidity standard of 0.5 McFarland in saline. Then, 100 μL of the obtained suspension of microorganisms was inoculated by the "lawn" method using a spatula on the Muller–Hinton medium. Solutions of silver nanoparticles were introduced into wells prepared in agar with a sterile metal punch. Further incubation was carried out for 24 h at 37 °C. Results: colloidal solution of silver nanoparticles at concentrations of 1%, 0.75%, 0.5% inhibited the growth of Str. agalacticae ATCC 3984 with a growth retardation zone of 6–7 mm. The E. faecalis ATCC 29212 strain was sensitive to solutions of silver nanoparticles at concentrations of 1%, 0.75%, 0.5% with a growth inhibition zone of 6–7 mm. Strain St. aureus 4785 demonstrated sensitivity to solutions of silver nanoparticles at concentrations of 1%, 0.75%, 0.5%, 0.1%, 0.05% with a growth retardation zone of 6–8 mm. Conclusion: colloidal solutions of silver nanoparticles have antimicrobial action against gram-positive bacteria (Str.agalacticae ATCC 3984, St. aureus ATCC 4785 , E. faecalis ATCC 29212) and yeast-like fungi of the genus Candida (C. albicans ATCC 10231, C. albicans 672 and C. albicans D-225M), but this action is strain-specific and depends on the concentration of the solution.

Green Ultrasound-Assisted Synthesis of Surface-Decorated Nanoparticles of Fe3O4 with Au and Ag: Study of the Antifungal and Antibacterial Activity

“…S. aureus produces infections, such as bacteremia, endocarditis, pneumonia, and sepsis. Similar inhibitory halos were obtained with both kinds of nanoparticles (Figure 6), suggesting a general way of action of this material, considering the cell wall differences presented between Gram-positive and -negative bacteria [13,51]. On the other hand, regarding the fungicide effect of Fe3O4 particles in eukaryotic cells, it is important to mention the fact that three Candida species were tested: C. albicans, C. parapsilosis, and C. glabrata, all human-life-threatening pathogens due to the high morbidity and mortality [52,53].…”

“…S. aureus produces infections, such as bacteremia, endocarditis, pneumonia, and sepsis. Similar inhibitory halos were obtained with both kinds of nanoparticles (Figure 6), suggesting a general way of action of this material, considering the cell wall differences presented between Gram-positive and -negative bacteria [13,51]. In this sense, it is important to note that the Bc value is nearly zero in three cases; consequently, a superparamagnetic behavior can be considered.…”

“…Furthermore, the key optical features of the Au nanoparticles, such as surface plasmon resonance (SPR), SERS, and luminescence, allow gold nanoparticles to be used as biosensors and biomarkers. Specifically, the sensing mechanisms of the biosensors based on Au nanoparticles are supported by an optical phenomenon associated with the interaction between the conduction band of the Ag nanoparticles and the light photons, resulting in a conjunct oscillation of the ultraviolet–visible band and the valence electrons (SPR) [ 13 , 14 , 15 ]. In the case of the SERS methodology, it is based on an amplification of Raman scattering of the specific molecules absorbed on the metallic nanostructures or rough metal surfaces [ 16 ].…”

Combined Effects of Anti-PD-L1 and Nanosonodynamic Therapy on HCC Immune Activation in Mice: An Investigation

“…This experiment underscores the low cytotoxicity of IR780@FOM-cRGD towards normal cells, demonstrating its favorable in vitro safety profile. 33 , 34 The hemolysis assay results demonstrated that the hemolysis rate remained below 10% for concentrations of IR780@FOM-cRGD up to 100 μg/mL ( Figure 3B ). This finding indicates that IR780@FOM-cRGD possesses favorable in vivo safety characteristic.…”