Evaluation of the mitochondrial system in the gonad-digestive gland complex of Biomphalaria glabrata (Mollusca, Gastropoda) after infection by Echinostoma paraensei (Trematoda, Echinostomatidae)

Tunholi, Victor Menezes; Tunholi-Alves, Vinícius Menezes; Santos, Anderson Teixeira; Garcia, Juberlan Silva; Maldonado, Arnaldo; da‐Silva, Wagner Seixas; Rodrigues, Maria de Lurdes de Azevedo; Pinheiro, Jairo

doi:10.1016/j.jip.2016.04.003

Cited by 10 publications

(3 citation statements)

References 40 publications

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“…However, grouped snails prefer to copulate and reproduce by cross-fertilization [28,29]. Significant advances in the knowledge of reproduction in B. glabrata have been made in the last decade, especially associated with qualitative histological, ultrastructural, and metabolically analysis after parasitic infection [30][31][32]. However, due to the increasing use of the B. glabrata as a model system in toxicology and ecotoxicology [19,33,34], the description of the gonadal histomorphometric parameters is important to complement the analyses with the qualitative histopathological biomarkers.…”

Section: Introductionmentioning

confidence: 99%

New Insights into the Gametogenesis of Biomphalaria glabrata (Mollusca, Gastropoda, Pulmonata): Implications for Histopathological Assessment

Rodrigues

Caixeta

Rocha

2021

Braz. arch. biol. technol.

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The reproductive system has a fundamental role in population dynamics and several reproduction strategies have been shaped by the environment over time. Many environmental pressures are generated by releasing pollutants, as endocrine disruptors, that can affect the reproductive system of individuals, among them invertebrates. The freshwater snails Biomphalaria spp. are used as biomonitor in several ecotoxicological studies; however, there are few studies about gametogenesis and morphology of reproductive snail cells, which could be used as a new biomarker. In this sense, the current study aims to characterize Biomphalaria glabrata gametogenesis, bringing new histomorphometric parameters for germinative cells. Results showed that the hermaphrodite tissue is formed by several acini with simple pavement epithelium with germinative and somatic cells. Oogenesis was classified into five developmental stages (OI to OV) according to diameter, nucleus area, total area, and follicular cell development, and then classified into previtellogenic and vitellogenic oocytes. The spermatogenesis was classified into spermatogonia (Spg), spermatocytes (Spc) and spermatids that were subdivided into five stages (Spt I to Spt V) according to cytoplasm losing, and nucleus spiralization along with Sertoli cells development. Thus, the HIGHLIGHTS  The gametogenesis of Biomphalaria glabrata was described. Oogenesis was classified into five developmental stages. New histomorphometric parameters were described for oocytes. Spermatogenesis was classified into seven stages of development. B. glabrata histomorphometric parameters as a tool for environmental and public health researches.2 Rodrigues, C.C.; et al.

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Section: Introductionmentioning

confidence: 99%

New Insights into the Gametogenesis of Biomphalaria glabrata (Mollusca, Gastropoda, Pulmonata): Implications for Histopathological Assessment

Rodrigues

Caixeta

Rocha

2021

Braz. arch. biol. technol.

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“…Tissue dissection of the cephalopodal mass (MC), digestive gland (GD), albumen gland (GA) and hemolymph collection (He) from the snails were performed according to the method of Tunholi et al (2016) and Vidal et al (2019), at the end of each evaluation week, with 17 snails from each experimental group (control and exposed; 24 and 48 h).…”

Section: Methodsmentioning

confidence: 99%

Influence of exposure Heterorhabditis bacteriophora HP88, (Rhabditida: Heterorhabditidae) on biological and physiological parameters of Pseudosuccinea columella (Basommatophora: Lymnaeidae)

Sperandio,

Tunholi,

Amaral

et al. 2023

Rev. Bras. Parasitol. Vet.

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Many studies about fasciolosis control have been carried out, whether acting on the adult parasite or in Pseudosuccinea columella, compromising the development of the larval stages. The present study aimed to evaluate, under laboratory conditions, the susceptibility of P. columella to Heterorhabditis bacteriophora HP88, during for 24 and 48 hours of exposure. The snails were evaluated for 21 days for accumulated mortality; number of eggs laid; hatchability rate; biochemical changes; and histopathological analysis. We found that exposure induced a reduction in glucose and glycogen levels, characterizing a negative energy balance, due to the depletion of energy reserves as a result of the direct competition established by the nematode/endosymbiont bacteria complex in such substrates. A mortality rate of 48.25% and 65.52% was observed in the group exposed for 24 h and 48 h, respectively, along with significant impairment of reproductive biology in both exposed groups in relation to the respective controls. The results presented here show that P. columella is susceptible to the nematode H. bacteriophora, with the potential to be used as an alternative bioagent in the control of this mollusk, especially in areas considered endemic for fascioliasis, in line with the position expressed by the World Health Organization Health.

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“…In vertebrates, organs such as the skeletal muscle, heart, liver, kidney, brain neurons, theca cells in the ovary, Leydig cells in the testis, and sperm cells have high concentrations of circular mitochondrial DNA (mtDNA) [12][13][14]. In invertebrates, flight muscle tissue and ommatidia in the compound eyes of insects, sperm cells, and gastrodermal cells in the digestive systems of some marine invertebrates, as well as the gills and digestive gland cells in mollusks, are rich in mitochondria and mtDNA [15][16][17]. Moreover, multiple circular genomes can exist within one mitochondrion.…”

Section: Introductionmentioning

confidence: 99%

Mining NCBI Sequence Read Archive Database: An Untapped Source of Organelle Genomes for Taxonomic and Comparative Genomics Research

Eldem,

Balcı

2024

Diversity

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The NCBI SRA database is constantly expanding due to the large amount of genomic and transcriptomic data from various organisms generated by next-generation sequencing, and re-searchers worldwide regularly deposit new data into the database. This high-coverage genomic and transcriptomic information can be re-evaluated regardless of the original research subject. The database-deposited NGS data can offer valuable insights into the genomes of organelles, particularly for non-model organisms. Here, we developed an automated bioinformatics workflow called “OrgaMiner”, designed to unveil high-quality mitochondrial and chloroplast genomes by data mining the NCBI SRA database. OrgaMiner, a Python-based pipeline, automatically orchestrates various tools to extract, assemble, and annotate organelle genomes for non-model organisms without available organelle genome sequences but with data in the NCBI SRA. To test the usability and feasibility of the pipeline, “mollusca” was selected as a keyword, and 76 new mitochondrial genomes were de novo assembled and annotated automatically without writing one single code. The applicability of the pipeline can be expanded to identify organelles in diverse invertebrate, vertebrate, and plant species by simply specifying the taxonomic name. OrgaMiner provides an easy-to-use, end-to-end solution for biologists mainly working with taxonomy and population genetics.

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Evaluation of the mitochondrial system in the gonad-digestive gland complex of Biomphalaria glabrata (Mollusca, Gastropoda) after infection by Echinostoma paraensei (Trematoda, Echinostomatidae)

Cited by 10 publications

References 40 publications

New Insights into the Gametogenesis of Biomphalaria glabrata (Mollusca, Gastropoda, Pulmonata): Implications for Histopathological Assessment

New Insights into the Gametogenesis of Biomphalaria glabrata (Mollusca, Gastropoda, Pulmonata): Implications for Histopathological Assessment

Influence of exposure Heterorhabditis bacteriophora HP88, (Rhabditida: Heterorhabditidae) on biological and physiological parameters of Pseudosuccinea columella (Basommatophora: Lymnaeidae)

Mining NCBI Sequence Read Archive Database: An Untapped Source of Organelle Genomes for Taxonomic and Comparative Genomics Research

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