Evaluation of the mutagenic potential of yangambin and of the hydroalcoholic extract of Ocotea duckei by the Ames test

Marques, Regina Célia Pereira; Medeiros, Sílvia Regina Batistuzzo de; Dias, Celidarque da Silva; Barbosa-Filho, José Maria; Agnez-Lima, Lucymara Fassarella

doi:10.1016/s1383-5718(03)00040-8

Cited by 71 publications

(37 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Assays on toxicity of the lignan yangambin in mice did not show any lethal effect up to 48 hours after the treatment, when using a 1 g/kg dose (Pachú et al, 1993). Recent studies on its mutagenic potential showed that this lignan at contents of up to 1.5 mg/ plate did not induce mutations of the bacterium Salmonella typhimurium (Marques et al, 2003). These data associated to the antileishmanial effect of the lignoid fraction and yangambin observed in the present work reinforce the therapeutic potential of this class of compounds.…”

Section: Resultssupporting

confidence: 86%

Crude Ethanolic Extract, Lignoid Fraction and Yangambin from Ocotea duckei (Lauraceae) Show Antileishmanial Activity

Monte

Barbosa

Sousa

et al. 2007

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Crude ethanolic extract, lignoid fraction and the purified compound yangambin were obtained from Ocotea duckei (Lauraceae) and their antileishmanial activity was tested against promastigote forms of Leishmania chagasi and Leishmania amazonensis cultivated in Schneider medium, supplemented with 20% of fetal bovine serum. All substances presented antileishmanial activity with IC 50 values of 135.7 μg/mL for the crude ethanolic extract, 26.5 μg/mL for the lignoid fraction and 49.0 μg/mL for yangambin on L. chagasi. For L. amazonensis the IC 50 values were 143.7 μg/mL, 48.2 μg/mL and 64.9 μg/mL for the crude ethanolic extract, the lignoid fraction, and the purified compound yangambin, respectively. The crude ethanolic extract, lignoid fraction, and yangambin caused an inhibition higher than Glucantime " , a reference drug used for the treatment of leishmaniasis.

show abstract

Section: Resultssupporting

confidence: 86%

Crude Ethanolic Extract, Lignoid Fraction and Yangambin from Ocotea duckei (Lauraceae) Show Antileishmanial Activity

Monte

Barbosa

Sousa

et al. 2007

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

show abstract

“…A sample was considered positive when the MI was equal to or greater than 2 for at least one of the tested doses and if it had a reproducible dose-response 21) . Several authors have used this parameter in their works [22][23][24][25] . According to Mortelmans and Zeiger 26) , in an important review of the Ames test a widely used approach is to analyze the results and to set a minimum fold increase, usually 2-3 folds, in revertants (over the solvent control) as a cut-off between mutagenic and nonmutagenic responses.…”

Section: Mutagenicity Testmentioning

confidence: 99%

Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents

Varella

Rampazo

Varanda

2008

Journal of Occupational Health

View full text Add to dashboard Cite

show abstract

“…Previous reports on Ocotea duckei described the isolation of alkaloids Silva et al, 2002;Morais et al, 1998a) and lignoids (Morais et al, 1996;Morais et al, 1998b;Morais et al, 1999;Barbosa-Filho et al, 1999). Yangambin, the main lignoid isolated from this species has shown many pharmacological properties, such as: (a) a selective platelet activating factor (PAF) receptor antagonist, observed in several in vitro and in vivo experimental models (Castro-Faria-Neto et al, 1995a,b;Herbert et al, 1997), (b) an effective pharmacological agent against cardiovascular collapse and mortality in endotoxin shock Ribeiro et al, 1996;Araújo et al, 2001), (c) an anti-allergic effect (Serra et al, 1997), (d) increased sleeping time induced by pentobarbital and the blockage of convulsions induced by pentylenotetrazole (Almeida et al, 1995;Pachú et al, 1993), (e) yangambin was not mutagenic when tested using strains of Salmonella typhimurium (Marques et al, 2003), (f) topical treatment of eggs and fi rst instars with yangambin as well as feeding larvae with a yangambin-treated diet resulted in inhibition of postembryonic development, morphological alteration, and oviposition reduction (Cabral et al, 2007a,b), (g) yangambin presented antileishmanial activity against promastigotes forms of L. chagasi and L. amazonensis (Monte-Neto et al, 2007) and antimicrobial activity against Escherichia coli (Antunes et al, 2006). This is the fi rst report of the composition of the essential oils obtained from the fruits, leaves, stem and roots of Ocotea duckei, as well as the pharmacological activity on the cardiovascular system.…”

Section: Introductionmentioning

confidence: 99%

GC-MS analysis and cardiovascular activity of the essential oil of Ocotea duckei

Barbosa-Filho¹,

Cunha²,

Dias³

et al. 2008

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO: "Análise CG-EM e atividade cardiovascular dos óleos essenciais de Ocotea duckei". O óleo essencial obtido da destilação por vapor de água das folhas, caule, raíz e frutos de Ocotea duckei teve sua composição química analisada através de CG-EM. A atividade farmacológica desses óleos também foi avaliada, mostrando signifi cantes efeitos cardiovasculares. Quarenta e nove substâncias foram identifi cadas, constituída por uma mistura complexa de monoterpenos (45%) e sesquiterpenos (55%). Os frutos forneceram (1,9%) mais óleo essencial do que os caules (1,0%), raízes (0,8%) e folhas (0,7%). O principal componente encontrado nas folhas foi o trans-cariofi leno (60,54%), nas cascas do caule, -eudesmol (27,51%) e nos frutos, dl-limoneno (30,12%). O componente predominante do óleo essencial das raízes foi elemol (24,31%). Em ratos normotensos, não anestesiados, o óleo essencial de diferentes partes de Ocotea duckei (folhas, frutos, caule e raiz) induziu signifi cante (p 0,05) hipotensão seguido de bradicardia. Unitermos:Ocotea duckei, Lauraceae, óleo essencial, análise CG-EM, atividade cardiovascular. ABSTRACT:The essential oils obtained by steam distillation from the roots, stems, leaves and fruits of Ocotea duckei had their composition analyzed by GC-MS. The pharmacological activity of these oils was also evaluated showing signifi cant cardiovascular effects. Forty-nine substances were identifi ed, consisting of a complex mixture of monoterpenes (45%) and sesquiterpenes (55%). The fruits yielded (1.9%) more essential oil than the stems (1.0%), roots (0.8%) and leaves (0.7%). The main component in the oil of the leaves was trans-caryophyllene (60.54%), in the stem bark -eudesmol (27.51%) and in the fruits, dl-limonene (30.12%). The predominant essential oil component in the roots was elemol (24.31%). In non-anaesthetized normotensive rats, the essential oils from different parts of Ocotea duckei (leaves, fruits, stem and roots) induced signifi cant (p < 0.05) hypotension followed by bradycardia.

show abstract

Evaluation of the mutagenic potential of yangambin and of the hydroalcoholic extract of Ocotea duckei by the Ames test

Cited by 71 publications

References 22 publications

Crude Ethanolic Extract, Lignoid Fraction and Yangambin from Ocotea duckei (Lauraceae) Show Antileishmanial Activity

Crude Ethanolic Extract, Lignoid Fraction and Yangambin from Ocotea duckei (Lauraceae) Show Antileishmanial Activity

Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents

GC-MS analysis and cardiovascular activity of the essential oil of Ocotea duckei

Contact Info

Product

Resources

About