Evaluation of the New Vitek 2 ANC Card for Identification of Medically Relevant Anaerobic Bacteria

Mory, F.; Alauzet, Corentine; Matuszeswski, Céline; Riegel, Philippe; Lozniewski, Alain

doi:10.1128/jcm.01778-08

Cited by 25 publications

(12 citation statements)

References 12 publications

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“…Conventional phenotypic identification of anaerobic bacteria to the species level can be difficult and laborious in the clinical microbiology laboratory. Commercial phenotypic systems like the Rapid ID 32A or Vitek 2 ANC card (bioMérieux, Marcy-l'Etoile, France) are faster but limited by the taxa included in the system database and, in most cases, only reliable to the genus level (6,8,11,12,14). In the last few years, the use of 16S rRNA gene sequencing as an alternative method for species identification has increased, while the time and costs (Ϸ$35 to $40) have been reduced (7,8,14,15).…”

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confidence: 99%

16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures

et al. 2010

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confidence: 99%

16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures

et al. 2010

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“…However, species and genera not present in the database had been eliminated from their study isolates (15,17). The lower level of correct identification obtained in our clinical study can be explained by the inclusion of test isolates that are not present in the database but are encountered in clinical material.…”

Section: Discussionmentioning

confidence: 68%

“…In contrast to other validations of the ANC card, Mory et al (15) and Rennie et al (17) previously reported correct specieslevel identifications for 86.5% and 95.1% of isolates, respectively. However, species and genera not present in the database had been eliminated from their study isolates (15,17).…”

Section: Discussionmentioning

confidence: 90%

“…This is in contrast to the methods used in two previously reported studies (15,17). The identification obtained by use of the Vitek 2 ANC system was compared with that obtained by use of 16S rRNA gene sequencing.…”

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confidence: 72%

“…In order to assess the accuracy of the ANC card in a "reallife" setting, unknown clinical isolates of anaerobic bacteria were used, regardless of whether the species are present in the Vitek database. This is in contrast to the methods used in two previously reported studies (15,17). The identification obtained by use of the Vitek 2 ANC system was compared with that obtained by use of 16S rRNA gene sequencing.…”

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Evaluation of the Vitek 2 ANC Card for Identification of Clinical Isolates of Anaerobic Bacteria

et al. 2011

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An evaluation of the Vitek 2 ANC card (bioMérieux, Marcy l'Etoile, France) was performed with 301 anaerobic isolates. Each strain was identified by 16S rRNA gene sequencing, which is considered to be the reference method. The Vitek 2 ANC card correctly identified 239 (79.4%) of the 301 clinical isolates to the genus level, including 100 species that were not represented in the database. Correct species identification was obtained for 60.1% (181/301) of the clinical isolates. For the isolates not identified to the species level, a correct genus identification was obtained for 47.0% of them (47/100), and 16 were accurately designated not identified. Although the Vitek 2 ANC card allows the rapid and acceptable identification of the most common clinically important anaerobic bacteria within 6 h, improvement is required for the identification of members of the genera Fusobacterium, Prevotella, and Actinomyces and certain Gram-positive anaerobic cocci (GPAC).The involvement of anaerobes in numerous and severe clinical infections has been reported (5, 13). The differences in antimicrobial susceptibility (9, 16) and the development of resistance to antimicrobial drugs (16) among anaerobic bacteria have been documented. Traditional methods for the identification of anaerobic pathogens are not always available in clinical bacteriology laboratories and are often laborious and time-consuming (12). Therefore, the need for a rapid and accurate method for the identification of anaerobic pathogens is highly desirable for appropriate treatment.In the last decades, different commercial enzyme kits for the identification of clinically relevant anaerobe isolates have been developed and evaluated, e.g., the RapID-ANA II panel, the Minitek systems, the Vitek ANI card, the BBL Crystal ANR ID kit, the API rapid ID 32 A system, and the API 20 A system (6-8, 10, 14, 18, 19).The new Vitek 2 ANC card (bioMérieux, Marcy l'Etoile, France) is designed to provide clinical laboratories with the capability for the rapid and accurate identification of clinically relevant anaerobic bacteria and Corynebacterium species. The card contains 64 microwells with 36 colorimetric enzymatic tests. The ANC database comprises 63 taxa of anaerobic bacteria and corynebacteria. Twenty genera are listed in the Vitek 2 ANC database: Actinomyces, Arcanobacterium, Bacteroides (Parabacteroides), Bifidobacterium, Clostridium, Collinsella, Corynebacterium, Eggerthella, Eubacterium, Finegoldia, Fusobacterium, Lactobacillus, Microbacterium, Parvimonas (formerly Micromonas), Peptoniphilus, Peptostreptococcus, Prevotella, Propionibacterium, Staphylococcus, and Veillonella. The system provides only a genus-level identification for Bifidobacterium sp. and Veillonella sp. Of the 36 biochemical profiles, 13 are fermentation tests, 17 are glycosidase and arylamidase tests, 2 are alkaline reactions, and 4 are other biochemical tests. Additional simple off-line tests, including cell morphology, Gram stain characteristic, and aerotolerance testing, are required to complete the i...

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Anaerobic Non‐Spore‐Forming Gram‐Positive Rods

Butler‐Wu,

She

2023

ClinMicroNow

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Anaerobic, Gram‐positive, non‐spore‐forming bacilli are widely distributed among the phyla Actinomycetota and Bacillota . The traditional genera Actinomyces , Actinobaculum , Actinotignum , Mobiluncus ,and Varibaculum all belong to the family Actinomycetaceae . The large degree of taxonomic heterogeneity among members of the genus Actinomyces has been recognized for some time. Many Eubacterium species are closely related to clostridia. Actinotignum schaalii is the species most frequently implicated in human disease among the genera Actinotignum and Actinobaculum . The Gram‐variable, curved Gram‐positive rods of the genus Mobiluncus can be detected in samples from the female vaginal tract. The cutaneous group of propionibacteria, now reclassified as Cutibacterium species, are members of the skin microbiota.

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Evaluation of the New Vitek 2 ANC Card for Identification of Medically Relevant Anaerobic Bacteria

Cited by 25 publications

References 12 publications

16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures

16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures

Evaluation of the Vitek 2 ANC Card for Identification of Clinical Isolates of Anaerobic Bacteria

Anaerobic Non‐Spore‐Forming Gram‐Positive Rods

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