Evaluation of the osteoblast response to a silica gel in vitro

Anderson, Susan; Downes, S.; Perry, Carole C.; Caballero, A. M.

doi:10.1023/a:1008955002955

Cited by 48 publications

(29 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[14][15][16][17] Finally, the use of scaffold material and surface modifications, such as a calcium phosphate coating, can play an important role in stimulating the osteogenic potential of stromal cells. [18][19][20] In view of the aforementioned, our previous studies with titanium fiber mesh indicated that culture time can be important for the final bone formation. Vehof et al 21 found in their study that cell-loaded titanium fiber mesh, without prolonged culturing, resulted in a very limited amount of bone after implantation.…”

Section: Introductionmentioning

confidence: 99%

Bone formation by rat bone marrow cells cultured on titanium fiber mesh: Effect of in vitro culture time

Dolder

Vehof

Spauwen³

et al. 2002

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

The objective of this study was to examine the effect of cell culture time on bone formation by rat bone marrow cells seeded in titanium fiber mesh. As a seeding technique, a high cell suspension was used (3 x 10(6) cells/mL). Therefore, 30 meshes were repeatedly rotated in a 10 mL tube (containing 30 x 10(6) cells) on a rotation plate (2 rpm) for 3 h. Osteogenic cells were cultured for 1, 4, and 8 days on titanium fiber mesh and finally implanted subcutaneously in rats. Meshes without cells were also implanted subcutaneously in rats. DNA and scanning electron microscopy (SEM) analyses and calcium measurements determined cellular proliferation and differentiation during the in vitro incubation period of the mesh implants. Four weeks after implant insertion, the animals were sacrificed. The implants, with their surrounding tissue, were retrieved and prepared for histologic evaluation and calcium measurements. DNA analysis of the in vitro experiment showed a lag phase from day 1 through day 4, but a 42% increase in DNA between days 4 and 8. SEM and calcium measurements indicated an increase in calcium from day 1 to day 4, yet only a small but significant increase from days 4 to 8. Histologic analysis demonstrated that bone was formed in all day 1 and day 4 implants, and that the bone-like tissue was present uniformly through the meshes. The bony tissue was morphologically characterized by osteocytes embedded in a mineralized matrix, with a layer of osteoid and osteoblasts at the surface. The day 8 implants showed only calcium phosphate deposition in the titanium fiber mesh. Calcium measurements of the implants revealed that calcification in day 1 implants was significantly higher (p < 0.05) compared to day 4 and day 8 implants. No significant difference in calcium content existed between day 4 and day 8 implants. On the basis of our results, we conclude that 1) bone formation was generated more effectively in osteogenic cells by a short culture time after seeding in titanium fiber mesh; 2) dynamic cell seeding is probably more effective than static cell seeding; and 3) selection of the right cells from the heterogenous bone marrow population remains a problem.

show abstract

Section: Introductionmentioning

confidence: 99%

Bone formation by rat bone marrow cells cultured on titanium fiber mesh: Effect of in vitro culture time

Dolder

Vehof

Spauwen³

et al. 2002

J. Biomed. Mater. Res.

View full text Add to dashboard Cite

show abstract

“…In addition, 45S5 ® Bioglass contains silica, a constituent that is lacking from the HA and TCP employed in this study. In previous studies characterizing the response of human osteoblasts seeded on silica surfaces without the interference of other ions present in glass ceramics, there were no apparent differences in cell number, metabolic activity, or ALP activity, yet nodule formation was accelerated on silica surfaces [40]. The importance of silica is confirmed in other studies demonstrating significant increases in ALP activity and type I collagen production by osteoblasts exposed to bioactive glasses with 46.1 mol% silica content (45S5) than cells exposed to bioactive glasses with 60 or 80 mol% silica (58S and 77S, respectively) [41].…”

Section: Resultsmentioning

confidence: 99%

Ceramic Identity Contributes to Mechanical Properties and Osteoblast Behavior on Macroporous Composite Scaffolds

Morales-Hernandez

Genetos

Working

et al. 2012

JFB

View full text Add to dashboard Cite

Implants formed of metals, bioceramics, or polymers may provide an alternative to autografts for treating large bone defects. However, limitations to each material motivate the examination of composites to capitalize on the beneficial aspects of individual components and to address the need for conferring bioactive behavior to the polymer matrix. We hypothesized that the inclusion of different bioceramics in a ceramic-polymer composite would alter the physical properties of the implant and the cellular osteogenic response. To test this, composite scaffolds formed from poly(lactide-co-glycolide) (PLG) and either hydroxyapatite (HA), β-tricalcium phosphate (TCP), or bioactive glass (Bioglass 45S®, BG) were fabricated, and the physical properties of each scaffold were examined. We quantified cell proliferation by DNA content, osteogenic response of human osteoblasts (NHOsts) to composite scaffolds by alkaline phosphatase (ALP) activity, and changes in gene expression by qPCR. Compared to BG-PLG scaffolds, HA-PLG and TCP-PLG composite scaffolds possessed greater compressive moduli. NHOsts on BG-PLG substrates exhibited higher ALP activity than those on control, HA-, or TCP-PLG scaffolds after 21 days, and cells on composites exhibited a 3-fold increase in ALP activity between 7 and 21 days versus a minimal increase on control scaffolds. Compared to cells on PLG controls, RUNX2 expression in NHOsts on composite scaffolds was lower at both 7 and 21 days, while expression of genes encoding for bone matrix proteins (COL1A1 and SPARC) was higher on BG-PLG scaffolds at both time points. These data demonstrate the importance of selecting a ceramic when fabricating composites applied for bone healing.

show abstract

“…In this connection, both metabolic role (a co-factor for prolyl hydroxylase) and a structural role (stabilization of collagen) have been attributed to Si [52,65,67]. Recent advancement in scientific techniques have revealed the true physiological role of Si in bone and connective tissue formation by observing the high concentrations of non-dialyzable Si in connective tissues and their components, and that apical accumulation of Si at the growing front of bone increased bone mineralization [70], and matrix polysaccharides and proteins synthesis with Si supplementation [52,65]. Furthermore, Si in the form of orthosilicic acid has been shown to induce type I collagen secretion and gene expression by osteoblasts and osteoblast-like cells for bone cell maturation and bone formation [71,72].…”

Section: Role Of Silicon In Animalsmentioning

confidence: 99%

Silicon: A Multitalented Micronutrient in OMICS Perspective – An Update

Zargar

Macha²,

Nazir

et al. 2012

View full text Add to dashboard Cite

Here we named silicon (Si) as "multitalented micronutrient". Silicon plays important role in inducing biotic and abiotic stress tolerance in plants. The transport of Si from soil has been studied in detail by numerous research groups and a number of Si transporter genes have been identified in different crop plants. However, there is still a need to mine potential candidate proteins and metabolites, altered due to the application of Si. Research on the impact of Si in animals is still in its infancy, but its impact on bone formation and remedy for osteoporosis has been examined in detail. In this review, we provide an updated-information on the role of Si in plant and animal physiology and metabolism. We also present our views on how OMICS-based approaches can elucidate the role of Si in understanding its involvement in the physiology and metabolism of crops and animals. SILICON: A MULTITALENTED MICRONUTRIENTSilicon (abbreviated Si, hereafter) is a micronutrient, but we would like to use the term -"a multitalented micronutrient". As the readers will go through the article, we hope that the reason and meaning behind its use will be clear. As far as the role of Si in crop improvement, and the major emphasis of this review, is concerned, we know much about its role in rice (Oryza sativa L.) -a genome model for cereal cropsthan any other crop plant. Plant systems in general are well studied with respect to Si role and function, however, there remains much to understand and investigate. On the other hand, Si role in animal systems is less understood, and has not been well studied; the research aspect in this direction is still in its infancy. In this update, we discuss the impact of Si on crop improvement and also its role in animal physiology and metabolism. We have also discussed its prospects based on omics-based research.To start with plants, it is well known that Si is a beneficial element, and is a provider of tolerance to crops / plants against various biotic and abiotic stresses. Reviewed earlier, Zargar and coworkers [1] provide information about Si role in providing tolerance to plants against various stresses.

show abstract

Evaluation of the osteoblast response to a silica gel in vitro

Cited by 48 publications

References 11 publications

Bone formation by rat bone marrow cells cultured on titanium fiber mesh: Effect of in vitro culture time

Bone formation by rat bone marrow cells cultured on titanium fiber mesh: Effect of in vitro culture time

Ceramic Identity Contributes to Mechanical Properties and Osteoblast Behavior on Macroporous Composite Scaffolds

Silicon: A Multitalented Micronutrient in OMICS Perspective – An Update

Contact Info

Product

Resources

About