Evaluation of the Osteointegration of a Novel Alkali-Treated Implant System &lt;i&gt;In Vivo&lt;/i&gt;

Kusumoto, Tetsuya; Yin, Derong; Zhang, Honghao; Chen, Luyuan; Nishizaki, Hiroshi; Komasa, Yutaka; Okazaki, Joji; Komasa, Satoshi

doi:10.2485/jhtb.26.355

Cited by 8 publications

(11 citation statements)

References 30 publications

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“…Hydrophilic surface treatments such as plasma treatment enhance the adhesion of BSA, vascular endothelial cells, and rBMMSCs. In cell-substance interactions, protein adsorption is one of the first events to occur between the implant material and the body when the implant is processed during implantation [ 65 , 66 , 67 , 68 , 69 ]. These results indicate that changes in surface structure are linked with initial adhesion and proliferation of various cells.…”

Section: Discussionmentioning

confidence: 99%

“…In vivo evaluation of material surfaces is essential for biomaterial development. We recently reported related results using an in vivo model with Sprague–Dawley rat femurs [ 68 , 69 , 70 , 71 ]. The rat femur model in the present study was used to evaluate the bone tissue response to implants via inclusion of the trabecular bone in the clinical context.…”

Section: Discussionmentioning

confidence: 99%

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Effects of Plasma Treatment on the Bioactivity of Alkali-Treated Ceria-Stabilised Zirconia/Alumina Nanocomposite (NANOZR)

Takao

Komasa

Agariguchi

et al. 2020

IJMS

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Zirconia ceramics such as ceria-stabilized zirconia/alumina nanocomposites (nano-ZR) are applied as implant materials due to their excellent mechanical properties. However, surface treatment is required to obtain sufficient biocompatibility. In the present study, we explored the material surface functionalization and assessed the initial adhesion of rat bone marrow mesenchymal stem cells, their osteogenic differentiation, and production of hard tissue, on plasma-treated alkali-modified nano-ZR. Superhydrophilicity was observed on the plasma-treated surface of alkali-treated nano-ZR along with hydroxide formation and reduced surface carbon. A decreased contact angle was also observed as nano-ZR attained an appropriate wettability index. Treated samples showed higher in vitro bovine serum albumin (BSA) adsorption, initial adhesion of bone marrow and endothelial vascular cells, high alkaline phosphatase activity, and increased expression of bone differentiation-related factors. Furthermore, the in vivo performance of treated nano-ZR was evaluated by implantation in the femur of male Sprague–Dawley rats. The results showed that the amount of bone formed after the plasma treatment of alkali-modified nano-ZR was higher than that of untreated nano-ZR. Thus, induction of superhydrophilicity in nano-ZR via atmospheric pressure plasma treatment affects bone marrow and vascular cell adhesion and promotes bone formation without altering other surface properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Plasma Treatment on the Bioactivity of Alkali-Treated Ceria-Stabilised Zirconia/Alumina Nanocomposite (NANOZR)

Takao

Komasa

Agariguchi

et al. 2020

IJMS

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“…Our results demonstrated that the TNS-modified titanium alloy disks were more hydrophilic and exhibited markedly improved wettability compared with unmodified surfaces. A better understanding of the surface roughness and topography of modified titanium alloy surfaces is needed to assess their wettability [ 32 , 33 , 34 ]. The nano-network structure formed on the titanium in the present study is similar to the hierarchical structure reported by Zhao et al [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…The experimental animals were randomly divided into two groups, with 10 rats in each group. The experimental method is based on the previous experience of our research group [ 11 , 32 ]. Animals were administered inhalation anesthesia, followed by intraperitoneal injection of anesthetics (1.5 mL/kg).…”

Section: Methodsmentioning

confidence: 99%

“…After tomographic acquisitions, the implant and surrounding tissue were reconstructed and analyzed using morphometric software (TRI/3D-BON; Ratoc System Engineering, Tokyo, Japan). The region of interest (ROI) was defined as the 500 μm-wide area of bone around the implants from 2 mm below the highest point of the growth plate to the distal 100 slices [ 32 ]. The bone volume fraction (BV/TV), mean trabecular number (Tb.N), mean trabecular thickness (Tb.Th), and mean trabecular separation (Tb.Sp) were calculated within the ROI.…”

Section: Methodsmentioning

confidence: 99%

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Effect of Amelogenin Coating of a Nano-Modified Titanium Surface on Bioactivity

Terada

Komasa

Kusumoto

et al. 2018

IJMS

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The interactions between implants and host tissues depend on several factors. In particular, a growing body of evidence has demonstrated that the surface texture of an implant influences the response of the surrounding cells. The purpose of this study is to develop new implant materials aiming at the regeneration of periodontal tissues as well as hard tissues by coating nano-modified titanium with amelogenin, which is one of the main proteins contained in Emdogain®. We confirmed by quartz crystal microbalance evaluation that amelogenin is easy to adsorb onto the nano-modified titanium surface as a coating. Scanning electron microscopy, scanning probe microscopy, X-ray photoelectron spectroscopy, and Fourier-transform infrared spectroscopy analyses confirmed that amelogenin coated the nano-modified titanium surface following alkali-treatment. In vitro evaluation using rat bone marrow and periodontal ligament cells revealed that the initial adhesion of both cell types and the induction of hard tissue differentiation such as cementum were improved by amelogenin coating. Additionally, the formation of new bone in implanted surrounding tissues was observed in in vivo evaluation using rat femurs. Together, these results suggest that this material may serve as a new implant material with the potential to play a major role in the advancement of clinical dentistry.

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Lactoferrin Coating Improves the Antibacterial and Osteogenic Properties of Alkali-Treated Titanium with Nanonetwork Structures

Yin

Hong

Chen

et al. 2020

Journal of Nanomaterials

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Titanium and its alloys are the main dental implant materials used at present. The biological properties of pure titanium can be further improved by surface treatment methods. Alkali treatment of pure titanium at room temperature can form nanonetwork structures (TNS) on the surface, which has better osteoinductive ability than pure titanium. However, TNS does not possess antimicrobial properties, and bacterial infection is one of the main reasons for the failure of dental implant therapy. Therefore, it was the focus of our research to endow TNS with certain antimicrobial properties on the premise of maintaining its osteoinductive ability. Because of its excellent broad-spectrum antimicrobial properties and because it promotes osteoblast-like cell growth, lactoferrin (LF) was considered a promising prospect as a surface biological treatment material. In this study, bovine LF of physiological concentration was successfully coated on the surface of TNS to form the TNS-LF composite material. Results from in vitro and in vivo experiments showed that TNS-LF had better osteoinductive ability than TNS. Bacterial attachment and biofilm formation were also significantly decreased on the surface of TNS-LF. Therefore, this study has provided an experimental basis for the development of osteoinduction-antimicrobial composite implant materials for dental applications.

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Evaluation of the Osteointegration of a Novel Alkali-Treated Implant System <i>In Vivo</i>

Cited by 8 publications

References 30 publications

Effects of Plasma Treatment on the Bioactivity of Alkali-Treated Ceria-Stabilised Zirconia/Alumina Nanocomposite (NANOZR)

Effects of Plasma Treatment on the Bioactivity of Alkali-Treated Ceria-Stabilised Zirconia/Alumina Nanocomposite (NANOZR)

Effect of Amelogenin Coating of a Nano-Modified Titanium Surface on Bioactivity

Lactoferrin Coating Improves the Antibacterial and Osteogenic Properties of Alkali-Treated Titanium with Nanonetwork Structures

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