Evaluation of the overall process on bioethanol production from miscanthus hydrolysates obtained by dilute acid pretreatment

Yoo, Hah Young; Yang, Xiaoguang; Kim, Dong Sup; Lee, Soo Kweon; Lotrakul, Pongtharin; Prasongsuk, Sehanat; Punnapayak, Hunsa; Kim, Seung Wook

doi:10.1007/s12257-016-0485-x

Cited by 26 publications

(18 citation statements)

References 33 publications

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“…Although C. glutamicum exhibits weak carbon catabolite repression toward mixed carbon sources, it cannot directly utilize complex polysaccharide polymers, such as xylan, cellulose, and starch, from lignocellulosic and starch biomass. To increase the economic feasibility of the biorefinery process, C. glutamicum has been engineered for the consolidated bioprocessing (CBP) of microalgal biomass and hemicellulose by the heterologous expression of amylolytic and cellulolytic enzymes . These recombinant C. glutamicum strains simultaneously hydrolyze biomass and produce biochemicals such as succinate, l ‐lysine, and xylonic acid using released glucose, maltose, cellobiose, d ‐xylose, and l ‐arabinose from biomass (Fig.…”

Section: Introductionmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Introductionmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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“…Recently, there has been significant interest in the biological degradation of lignocelluloses, some of the most abundant reusable resource in nature, due to their potential application to biorefinery. Forestry and agricultural residues (plant biomass) are composed of cellulose, hemicellulose, and lignin, and the potential sugars portions (cellulose and hemicellulose) can be converted to value‐added products by a bioconversion process . Hemicellulose is present in the plant cell wall, and comprises one of the major renewable biomass resources.…”

Section: Introductionmentioning

confidence: 99%

Prospects for Bio‐Industrial Application of an Extremely Alkaline Mannanase FromBacillus subtilissubsp.inaquosorumCSB31

Regmi

Yoo

Choi

et al. 2017

Biotechnology Journal

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Mannan-degrading enzymes have been growing interest in bio-industrial applications, such as the pulp and paper, food, and pharmaceutical industries. In this study, an extremely alkaline mannanase (MnB31) is produced by Bacillus subtilis subsp. inaquosorum CSB31. MnB31 is purified to 17.92-fold with a 21.51% yield and specific activity of 1,796.13 U mg by anion-exchange and gel filtration column chromatography. The biochemical characterization of MnB31 is performed, and the results are as follows: molecular weight of ≈47 kDa with an optimum temperature of 60 °C and pH of 12.5. The enzyme is strongly activated by Co , Mn , Na , and K , and inhibited by Zn , Ni , and Mg . Halo-tolerance (10% NaCl), urea stability (3 M), and protease resistance are also observed. The kinetic parameters of MnB31 are found to be K of 0.043 mg ml , and V of 1,046 ± 3.605 U mg , respectively. In addition, the thermodynamical parameters are investigated; the activation energy (E ) is found to be 31.36 kJ mol with a K value of 156.9 × 10 s , ΔH (28.59 kJ mol ), ΔG (42.38 kJ mol ), ΔS (-41.39 J mol K ), Q (1.40), ΔG (-8.697 kJ mol ), and ΔG (-48.22 kJ mol ). These results suggest that MnB31 has potential bio-industrial application, due to its greater hydrolytic efficiency and feasibility of enzymatic reaction.

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“…Lignocellulosic biomass, such as wood, grasses, forest and agricultural residues, has been highlighted as having great potential for bioenergy production since it is inedible and the most abundant resource in the world. As a promising energy crop, Miscanthus (Lee and Kuan 2015 ; Yoo et al 2016 ), Poplar (Sannigrahi et al 2010 ) and switchgrass (David and Ragauskas 2010 ) have received attention due to their advantages including high yield of biomass per unit planted area, ability to grow without pesticides or fertilizers, low maintenance and long lifespan. Switchgrass ( Panicum viragatum ), a perennial warm season C4 grass and a dominant species of the remnant tall grass prairies, has gained increasing attention for use in biofuel production because relatively low energy input is required for production and a high biomass yield.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

et al. 2017

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ObjectiveTo develop and prototype a high-throughput microplate assay to assess anaerobic microorganisms and lignocellulosic biomasses in a rapid, cost-effective screen for consolidated bioprocessing potential.Results Clostridium thermocellum parent Δhpt strain deconstructed Avicel to cellobiose, glucose, and generated lactic acid, formic acid, acetic acid and ethanol as fermentation products in titers and ratios similar to larger scale fermentations confirming the suitability of a plate-based method for C. thermocellum growth studies. C. thermocellum strain LL1210, with gene deletions in the key central metabolic pathways, produced higher ethanol titers in the Consolidated Bioprocessing (CBP) plate assay for both Avicel and switchgrass fermentations when compared to the Δhpt strain.ConclusionA prototype microplate assay system is developed that will facilitate high-throughput bioprospecting for new lignocellulosic biomass types, genetic variants and new microbial strains for bioethanol production.

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Evaluation of the overall process on bioethanol production from miscanthus hydrolysates obtained by dilute acid pretreatment

Cited by 26 publications

References 33 publications

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Prospects for Bio‐Industrial Application of an Extremely Alkaline Mannanase FromBacillus subtilissubsp.inaquosorumCSB31

Anaerobic microplate assay for direct microbial conversion of switchgrass and Avicel using Clostridium thermocellum

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