Evaluation of the Pan Influenza detection kit utilizing the PLEX-ID and influenza samples from the 2011 respiratory season

Abstract

A comparison study was performed between the PLEX-ID and the CDC RT-PCR method for the detection and identification of Influenza A viruses using nasopharyngeal samples (N = 75) collected between January and May 2011. Overall agreement was 89.3% (67/75 kappa = 0.57 95% CI 0.3–0.89). Positive percent agreement was 92.3% (60/65); negative percent agreement was 70% (7/10). H1N1 pdm09 identified: 42.6% (32/75) and 54.7% (41/75) by PLEX-ID and CDC RT-PCR, respectively. H3N2 identified: 29.3% (22/75) and 32% (24/75) of samples by PLEX-ID and CDC RT-PCR, respectively. Negatives identified: 16% (12/75) and 13.3% (10/75), by PLEX-ID and CDC RT-PCR respectively. For influenza viruses identified as H1N1 pdm09, Influenza A virus A/NEW YORK/15/2009(H1N1 pdm09) was the most prevalent genotype at 50% (16/32), followed by A/CALIFORNIA/05/2009(H1N1 pdm09) at 18.2% (6/32). Updated assay plates containing additional primers designed for H1N1 pdm09 HA and NA genes were utilized for this evaluation. Among H1N1 pdm09 samples, the HA gene was conserved in 96.9% (31/32) of samples. The NA gene was conserved in 96.9% (31/32).

A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

“…Despite numerous limitations to the study, the results presented here add additional performance characteristic information to previously published studies that utilized RT-PCR/ESI-MS for the detection and identification of respiratory viruses ( Deyde et al, 2010 , Deyde et al, 2011 , Sampath et al, 2007 , Tang et al, 2013 , Murillo et al, 2013 ).…”

“…Reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry (RT-PCR/ESI-MS) technology is a highly complex technology that provides accurate diagnostic information for respiratory viruses, particularly influenza A ( Chen et al, 2011a , Chen et al, 2011b , Deyde et al, 2010 , Deyde et al, 2011 , Forman et al, 2012 , Jeng et al, 2012 , Sampath et al, 2007 , Tang et al, 2013 , Murillo et al, 2013 ).…”

Improving the Diagnosis of Bloodstream Infections: PCR Coupled with Mass Spectrometry

“…For instance, genotyping of Staphylococcus aureus [41,42], Acinetobacter baumannii [43][44][45], and respiratory pathogens [46,47] [50][51][52]. Moreover, this technology shows a great promise for the global surveillance of influenza virus [53][54][55]. Remarkably, it was able to detect the novel H1N1 strain during the 2009 influenza virus outbreak without any modification in the Influenza Surveillance Assay (Ibis Biosciences, Carlsbad, CA, USA) [56].…”

Advance detection technologies for select biothreat agents

“…PLEX-ID is a powerful technology that combines the power of both PCR and mass spectrometry (Murillo et al, 2013). PLEX-ID allows for rapid identification and genotyping of microorganisms including, bacteria, viruses, fungi, etc.…”