Evaluation of the Physicochemical and Biological Properties of EndoSequence BC Sealer HiFlow

Mann, Akshita; Zeng, Yu; Kirkpatrick, Timothy C.; Hoeven, Ransome van der; Silva, Renato; Letra, Ariadne; Souza, Letícia Chaves de

doi:10.1016/j.joen.2021.10.001

Cited by 27 publications

(29 citation statements)

References 44 publications

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“…Both calcium silicate cement‐based sealers (ESbcs and AHPbcs) exhibited an adequate cytocompatibility compared with a negative control group. These results are in accordance with previous studies on the biological properties of calcium silicate cement‐based sealers as a group of dental materials (Mann et al, 2022; Park et al, 2021; Zordan‐Bronzel et al, 2019). The main components tricalcium silicate and a radiopacifying agent (zirconium oxides) have been shown to be biocompatible in previous studies (Campi et al, 2022).…”

Section: Discussionsupporting

confidence: 93%

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

et al. 2022

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Aim To assess the cytocompatibility and bioactive potential of the new calcium silicate cement‐based sealer AH Plus Bioceramic Sealer (AHPbcs) on human periodontal ligament stem cells (hPDLSCs) compared with the epoxy resin‐based sealer AH Plus (AHP) and the calcium silicate cement‐based sealer Endosequence BC Sealer (ESbcs). Methodology Standardized sample discs and 1:1, 1:2 and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, cell migration/proliferation via a wound‐healing assay, osteo/cemento/odontogenic marker expression via RT‐qPCR and cell mineralized nodule formation via Alizarin Red S staining. HPDLSCs were isolated from extracted third molars. Comparisons were made with hPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media. Statistical significance was established at p < .05. Results A higher peak of Ca2+ was detected from ESbcs compared with AHPbcs and AHP in SEM–EDX. Both AHPbcs and ESbcs showed significantly positive results in the cytocompatibility assays (cell viability, migration/proliferation, attachment and morphology) compared with a negative control group, whilst AHP showed significant negative results. Both AHPbcs and ESbcs exhibited an upregulation of at least one osteo/odonto/cementogenic marker compared with the negative and positive control groups. Both ESbcs and AHPbcs showed a significantly higher calcified nodule formation than the negative and positive control groups, indicative of their biomineralization potential and were also significantly higher than AHP group. Conclusion AH Plus Bioceramic Sealer exhibited a significantly higher cytocompatibility and bioactive potential than AH Plus and a similar cytocompatibility to that of Endosequence BC Sealer. Endosequence BC Sealer exhibited a significantly higher mineralization potential than the other tested sealers. The results from this in vitro study act as supporting evidence for the use of AH Plus Bioceramic Sealer in root canal treatment.

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Section: Discussionsupporting

confidence: 93%

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

et al. 2022

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“…However, high solubility also means that the material dissolves quickly, creating gaps in the canals and generating reinfection [ 34 ]. This was also observed in other studies about bioceramic sealers [ 35 ] and could be explained by the presence of fine hydrophilic particles in the composition of these sealers, which generate an increase in surface area that may increase sealer solubility when in contact with water/moisture, [ 36 ]. Therefore, it is essential to determine clinically the rate at which Bio-CP dissolves, since there is no Gutta Percha to complement the sealer in the root canal treatment of primary teeth.…”

Section: Discussionsupporting

confidence: 71%

Physicochemical and Antibacterial Properties of Conventional and Two Premixed Root Canal Filling Materials in Primary Teeth

Hachem

Chedid

Nehmé

et al. 2022

JFB

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In this study, some physicochemical and antibacterial properties of three root canal filling materials for primary teeth, Calplus “CP” (Prevest DenPro, Lewes, DE, USA), Bio-C Pulpecto “Bio-CP” (Angelus, Basil, Londrina, Paraná, Brazil), and Zinc Oxide and Eugenol “ZOE” (Prevest DenPro, Lewes, DE, USA) were compared. For each material, the pH, solubility, contact angle, and crystalline microstructure under SEM were evaluated. Their antibacterial activity against Enterococcus faecalis was determined through direct tests. The Kruskal–Wallis test was used to analyze the results using a one-way analysis of variance on ranks. All the materials had an alkaline pH at 3, 24, and 72 h, with CalPlus having the highest (p < 0.05). Bio-CP was more soluble during the evaluation period (24 h) than ZOE and CalPlus (p < 0.05). Bio-CP and ZOE demonstrated the creation of crystallite structures on their surfaces after immersion in PBS at 37 °C, whereas CalPlus showed none. The lowest contact angle was observed for Bio-CP (53 ± 1.5°); contact angles of (86 ± 4°) and (96 ± 1°), respectively, were observed after 10 s of the deposition of the water drop for CalPlus and ZOE. In conclusion, according to this study, there is still a need to develop new filling materials for primary teeth. ZOE, CalPlus and Bio-CP demonstrated different physicochemical and antibacterial properties, but none of the materials had optimal properties and could be considered the most suitable filling material for primary teeth pulpectomy. Bioceramics in their current state are not an alternative. The physicochemical and antibacterial properties still need improvement to fit the intricate anatomy of primary teeth.

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“…Two endodontic sealers, described in Table 1 with their compositions, working time, setting time, and radiopacity, were used [4,10,[23][24][25][26][27]. These sealers are the most commonly used epoxy resin and tricalcium silicate products, although not the only ones with epoxy or tricalcium silicate matrices.…”

Section: Methodsmentioning

confidence: 99%

Use of micro-CT to examine effects of heat on coronal obturation

Shen

Daniel

et al. 2022

J Oral Sci

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Purpose:The aim of this study was to categorize the effects of heat on coronal obturation with gutta-percha and sealer using X-ray micro-computed tomography (micro-CT). Methods: Ten single-rooted, extracted human teeth were shaped using ProTaper NEXT files to size X5 (#50/Taper 6%) with 2.5% NaOCl irrigation. A single ProTaper NEXT X5 gutta-percha point was then inserted with epoxy resin (AH Plus) or tricalcium silicate (EndoSequence BC) sealer (n = 5/group), and cut at the cemento-enamel junction. The teeth were scanned using micro-CT (SkyScan1272) to obtain 11 sagittal 2-D images. Three calibrated raters categorized the coronal 0.5 mm of the images into four categories: "swirled sealer and/or gutta-percha without voids" (I), "uniform voids and sealer/gutta-percha" (II), "non-uniform voids and sealer/gutta-percha" (III), and "swirled sealer and/or guttapercha with voids" (IV). Intra-rater and inter-rater reliability were then calculated. Chi-square tests were conducted to determine the significance of differences in each category between sealers. Results:The intra-class correlation coefficient was 0.55 (same rater/two different times) and Fleiss' kappa (different raters/same image) was 0.34.

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Evaluation of the Physicochemical and Biological Properties of EndoSequence BC Sealer HiFlow

Cited by 27 publications

References 44 publications

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

Cytocompatibility and bioactive potential of AH Plus Bioceramic Sealer: An in vitro study

Physicochemical and Antibacterial Properties of Conventional and Two Premixed Root Canal Filling Materials in Primary Teeth

Use of micro-CT to examine effects of heat on coronal obturation

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