Current operations within wastewater
treatment plants (WWTPs),
particularly the anaerobic digestion and activated sludge processes,
can be tapped for production of microbial lipids. This can be accomplished
by directing the anaerobic digestion to produce short chain carboxylic
acids (SCCAs) that can then be used as substrates for lipid production
using activated sludge microbial consortium in an add-on aerobic lipid
production unit (similar to an activated sludge process). This study
was conducted to assess the suitability of a Test Medium (previously
proven suitable for aerobic lipid production in activated sludge)
for the proposed integrated system. Results showed that, when used
for anaerobic fermentation, the Test medium produced 0.48 g-SCCAs/g-substrate
that includes acetic, butyric, and predominantly (∼89 wt%),
lactic acid. Subsequently, the Test Medium resulted to a peak lipid
yield of ∼0.17 g/g-SCCAs. Complete substrate consumptions were
observed without pH control when the Test Medium was used for both
bioprocesses. These results suggest the suitability of the Test Medium
for the integrated system for microbial lipids production. This could
significantly simplify the implementation of the proposed integrated
system, which could provide the basis for future urban biorefineries
through existing WWTPs.