Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect

Abstract

Probiotic ingestion is recommended as a preventive approach to maintain the balance of the intestinal microbiota and to enhance the human well-being. During the whole life of each individual, the gut microbiota composition could be altered by lifestyle, diet, antibiotic therapies and other stress conditions, which may lead to acute and chronic disorders. Hence, probiotics can be administered for the prevention or treatment of some disorders, including lactose malabsorption, acute diarrhoea, irritable bowel syndrome, necrotizing enterocolitis and mild forms of inflammatory bowel disease. The probiotic-mediated effect is an important issue that needs to be addressed in relation to strain-specific probiotic properties. In this work, the probiotic properties of new Lactobacillus and Bifidobacterium strains were screened, and their effects in vitro were evaluated. They were screened for probiotic properties by determining their tolerance to low pH and to bile salts, antibiotic sensitivity, antimicrobial activity and vitamin B8, B9 and B12 production, and by considering their ability to increase the antioxidant potential and to modulate the inflammatory status of systemic-miming cell lines in vitro. Three out of the examined strains presenting the most performant probiotic properties, as Lactobacillus plantarum PBS067, Lactobacillus rhamnosus PBS070 and Bifidobacterium animalis subsp. lactis PBSO75, were evaluated for their effects also on human intestinal HT-29 cell line. The obtained results support the possibility to move to another level of study, that is, the oral administration of these probiotical strains to patients with acute and chronic gut disorders, by in vivo experiments.

Microencapsulation of new probiotic formulations for gastrointestinal delivery: in vitro study to assess viability and biological properties

“…In a previous work (Presti et al 2015), an evaluation of the resistance of nonencapsulated lactobacilli and bifidobacteria strains in simulated intestinal juice (SIJ, bile salts and pancreatine solution) was performed. The three examined strains showed a significant resistance to bile salts, with negligible loss of viability upon exposure to SIJ at 37°C for 3 h (data not shown), while a consistent loss of viability was observed at low pH values.…”

“…lactis PBS075 (DSM 25566) supplied from a private collection previously identified and characterised (Presti et al 2015).…”

Intestinal microbiota: updated evidence-based data on the efficacy of Lactobacillus rhamnosus GG and Bifidobacterium longum in pediatric practice

“…Установлено, что эффекты этих препаратов являются штаммспецифичными, т. е. определяются свойствами кон-кретного штамма [37].…”

Ontogenesis and Dysontogenesis of the Gut Microbiota in Young Children: a Trigger Mechanism of Child Health Disorders

“…Установлено, что эффекты этих препаратов являются штаммспецифичными, т. е. опреде-ляются свойствами конкретного штамма [73].…”