The research aims to investigate the effect of Fe3O4-NPs and it's conjugated with chitosan nanoparticles on the immunological indicators, such as IgA, IgG, and interleukin-6, and the iron indicators, of Ferritin, Transferrin, TIBC, and Iron in the serum of male albino rats induced with anemia by phenyl-hydrazine. The rats were separated into six groups in five replicates, each of which included (T1) the control group, (T2) anemia induced group, T3 group animals given Labneh enrichment with 2.5 mg of Fe3O4-NPs ml/day, T4 group animals given Labneh enrichment with 2.5 mg of Ch-NPs ml/day, T5 group animals given Labneh enrichment with 2.5 mg of each Fe3O4-NPs conjugated with Ch-NPs ml/day, T6 group animals given Labneh enrichment with 2.5 mg of Hamvir ml/day, and were fed for 30 days. The results showed a change and increase in the values of free fatty acids, as they reached their highest value after 20 days of preservation, where the treatment (T1) recorded a noticeable increase, reaching (2.31). Likewise, in the treatment (T5), we notice a significant increase in it, as it reached (0.75), in contrast to the rest. All coefficients that were (T2), (T3) and (T4) recorded (0.53), (0.58) and (0.63), respectively. the values of RBCs and Hb, which gave a significant decrease and it was at 2.84 (1×106/ ml) and 6.4 (g/dl), respectively, compared to the control treatment (T1).