Evaluation of the Reproductive Toxicity of Chlorpyrifos Methyl, Diazinon and Profenofos Pesticides in Male Rats

Zidan, Nour El-Hoda A.

doi:10.3923/ijp.2009.51.57

Cited by 50 publications

(37 citation statements)

References 29 publications

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“…However, the CPF group had the lowest FSH concentration when compared to other groups. This observation agreed with the previous work that pesticides, including CPF lowered the FSH level (Zidan, 2009;Mandal and Das, 2011;Uslu et al, 2013;Shittu et al, 2013). FSH, a member of pituitary glycoprotein, plays a dual role in spermatogenesis by directly affecting the sertoli cells to stimulate and initiate germ cell number and indirectly, enhances androgen production by the Leydig cells (O'Shaughnessy et al, 2010).…”

Section: Discussionsupporting

confidence: 82%

“…The reduced concentration of FSH and LH concentrations in the CPF group agreed with those recorded by previous workers following pesticide exposure (Zidan, 2009). This observation may be related to the inhibitory effect on genes, involved in gonadotropin synthesis, or interference with steroidogenesis (Liu et al, 2012).…”

Section: Science Publicationssupporting

confidence: 77%

“…Decrease in testosterone concentration may have occurred due to direct damage to the Leydig cells (Zidan, 2009). The increased MDA concentration indicates lipoperoxidative changes, hence possible oxidative damage to the testicular tissues, including the Leydig cells.…”

Section: Science Publicationsmentioning

confidence: 99%

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AMELIORATIVE EFFECT OF <i>HIBISCUS SABDARIFFA</i> LINN ON SUBCHRONIC CHLORPYRIFOS-INDUCED ALTERATIONS IN SEX AND THYROID HORMONES IN MALE WISTAR RATS

Shittu

Aisha

Ambali

et al. 2014

American Journal of Pharmacology and Toxicology

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Studies have shown that Chlorpyrifos (CPF), an Organophosphate (OP) insecticide alters both sex and thyroid hormones. Apart from inhibiting Acetylcholinesterase (AChE) activity, CPF has been shown to cause oxidative stress. The antioxidant potentials of many flavonoid-containing plants are increasingly being exploited in the therapy of many infectious and non-infectious diseases. Hibiscus Sabdariffa (HS) is one of the most widely used nutraceuticals that has been used traditionally to combat various illnesses due to its high flavonoid contents. The present study was therefore aimed at evaluating the ameliorative potentials of HS on subchronic chlorpyrifos-evoked alterations in sex and thyroid hormones in male Wistar rats. Forty-two (42) young adult male Wistar rats were divided at random into six groups containing seven (7) rats per group. Group I was administered distilled water (2 mL kg −1 ) only while group II received soya oil (2 mL kg −1 ), Group III was dosed with only aqueous extract of HS (500 mL kg −1 ~ 1/10 th of the LD 50 ), while group IV was given CPF (10.6 mL kg −1 ~ 1/8 th of the LD 50 ). Group V was pretreated with low dose of HS (250 mg kg −1 ~ 1/20 th of the LD 50 ) and then administered reconstituted CPF (10.6 mg kg −1 ), 30 min later. Group VI was pretreated with high dose of the HS (500 mg kg −1 ) and then administered CPF (10.6 mg kg −1 ), 30 min later. The regimens were administered orally by gavage once daily for a period of 11 weeks. At the end of the treatment period, sera obtained from the blood samples were analyzed for the levels of Follicle-Stimulating Hormone (FSH), Luteinizing Hormone (LH), testosterone, thyroid hormones (T 3 , T 4 and TSH) and AChE. Thyroid and pituitary glands of each rat were also evaluated for Malondialdehyde (MDA) concentration. Results showed a non-significant (p>0.05) decrease in the concentrations of FSH, LH and testosterone in the CPF group relative to the other groups. There was significant decrease (p<0.05) in the concentrations of T 3, T 4 and an increase in TSH in the CPF group relative to the other groups. There were significant increases (p<0.05) in MDA concentrations in the thyroid and pituitary glands in the CPF group compared to the other groups. Pretreatment with aqueous extract of HS demonstrated a dose-dependent amelioration of CPF-induced alterations in the levels of testosterone, LH, FSH, AChE, T 3 , T 4 and TSH in the serum and that of pituitary and thyroid glands MDA. This may be partly due to its high level of polyphenolic compounds that confer its antioxidant and possibly AChE restoration AJPTactivities. It is therefore concluded that pretreatment of individuals who are occupationally exposed to CPF and probably other OPs with the extract of HS may result in protection from the insecticideinduced adverse reproductive health outcomes.

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Section: Discussionsupporting

confidence: 82%

Section: Science Publicationssupporting

confidence: 77%

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AMELIORATIVE EFFECT OF <i>HIBISCUS SABDARIFFA</i> LINN ON SUBCHRONIC CHLORPYRIFOS-INDUCED ALTERATIONS IN SEX AND THYROID HORMONES IN MALE WISTAR RATS

Shittu

Aisha

Ambali

et al. 2014

American Journal of Pharmacology and Toxicology

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“…Oxidative stress may therefore be partly responsible for the alteration in the synthesis of some sex hormones and other substances essential for sustaining reproductive activity evoked by CPF and other OPs [11,12] , culminating in low sperm count. Therefore, oxidative stress may have been partly involved in the male reproductive toxicity reported in CPF poisoning [11,12] .…”

Section: Discussionmentioning

confidence: 99%

“…Association between low sperm concentration and serum concentrations of 3,5,6-trichloropyridinol, a metabolite of CPF has been reported in humans [10] . Similarly, studies using animal models have demonstrated reduced sperm concentrations and alterations in sex hormone following repeated CPF exposure [11,12] . There are several possible mechanisms for the anti-gonadal actions of OPs: they may exert a direct inhibitory action on the testis; they may affect the pituitary, causing changes in gonadotrophin concentrations and thus subsequent spermatogenic impairment; or they may change the concentration of neurotransmitters [13] .…”

Section: Introductionmentioning

confidence: 99%

Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in Wistar rats

Umosen

Ambali

Ayo

et al. 2012

Asian Pacific Journal of Tropical Biomedicine

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Molecular interactions of chlorpyrifos and its environmental degradation products with human sex hormone‐binding globulin: an in silico study

Hazarika

Ganguly

Mahanta

2019

J of Applied Toxicology

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In recent years, there has been a widespread interest and awareness about health issues posed by endocrine‐disrupting chemicals present in the environment. These chemicals, often present in food and many consumer products, can interfere with hormone biosynthesis and metabolism and may result in deviation from normal homeostatic control. Chlorpyrifos (CPF), a major endocrine‐disrupting chemical is used worldwide as an agricultural insecticide against a broad spectrum of insect pests in rice cultivation and to control termites. The insecticide mostly undergoes environmental degradation to chlorpyrifos‐oxon (CPYO), des‐ethyl chlorpyrifos (DEC), 3,5,6‐trichloro‐2‐methoxypyridine (TMP) and 3,5,6‐trichloro‐2‐pyridinol (TCP). Results from several epidemiological studies suggest that exposure to CPF can result in reproductive disorders, including infertility in male and female. Sex hormone‐binding globulin (SHBG) is a circulatory protein that binds sex steroids and is a potential target for endocrine disruptors in the human body. The objective of the present study involved computational approaches to apprehend the mechanism of molecular interaction of CPF and its four degradation products (CPYO, DEC, TMP, TCP) with human SHBG using molecular docking simulation. All five compounds (CPF, CPYO, DEC, TMP, TCP) showed high binding affinity with SHBG; however, the binding affinity values were higher (more negative) for CPF, CPYO, DEC and TMP than for TCP indicating that CPF, CPYO, DEC and TMP formed a tight interaction with SHBG. From the results obtained with the docking analysis, it can be opined that CPF, CPYO, DEC and TMP could possibly act as potential endocrine disruptors for androgen signaling.

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Evaluation of the Reproductive Toxicity of Chlorpyrifos Methyl, Diazinon and Profenofos Pesticides in Male Rats

Cited by 50 publications

References 29 publications

AMELIORATIVE EFFECT OF <i>HIBISCUS SABDARIFFA</i> LINN ON SUBCHRONIC CHLORPYRIFOS-INDUCED ALTERATIONS IN SEX AND THYROID HORMONES IN MALE WISTAR RATS

AMELIORATIVE EFFECT OF <i>HIBISCUS SABDARIFFA</i> LINN ON SUBCHRONIC CHLORPYRIFOS-INDUCED ALTERATIONS IN SEX AND THYROID HORMONES IN MALE WISTAR RATS

Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in Wistar rats

Molecular interactions of chlorpyrifos and its environmental degradation products with human sex hormone‐binding globulin: an in silico study

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