Evaluation of the salivary antibody response to Helicobacter pylori

Shaw, Robert D.; Roeske, Maxwell J; Rubenbauer, F.; Marchildon, Patrice A.; Cheng, Edward H.; Peacock, Jeff

doi:10.1016/s0016-5085(98)84406-5

Cited by 2 publications

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“…At the present time antibody testing typically requires venepuncture followed by storage and processing of serum samples; this has prompted the search for simpler methods of sample collection for IgG antibody testing. Other means of simplified diagnosis that have previously undergone evaluation include rapid whole blood tests and saliva tests for IgG antibodies to H. pylori 6 –14 . In our study, we evaluated the technique of collection of a single drop of blood by fingerstick lancet as an alternative to collection of a blood specimen by venepuncture.…”

Section: Discussionmentioning

confidence: 99%

Use of a dried plasma collection card for simplified diagnosis of Helicobacter pylori infection

Boes²,

1999

Aliment Pharmacol Ther

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Objectives : To compare testing for Helicobacter pylori IgG antibodies using a dried plasma collection card device with specimens obtained by venepuncture. Methods : Eighty‐four patients underwent testing for H. pylori IgG antibodies by venepuncture and by fingerstick using a single drop of blood placed on each of two dried plasma collection card devices. The correlation of venepuncture results to dried plasma card results was assessed. Results : There was a high degree of correlation of EIA results between venepuncture and dried plasma card specimens (r=0.98). The qualitative result of the first dried plasma card and venepuncture specimen testing differed in 7 of 84 patients and for the second dried plasma card differed in 7 of 82 patients. The first dried plasma card was 93% sensitive and 100% specific and the second was 93% sensitive and 98% specific as compared to the venepuncture result. There was a high degree of correlation between the first and second dried plasma cards (r=0.996). Conclusions : The dried plasma collection card has adequate sensitivity and excellent specificity as compared to venepuncture specimens and is a feasible alternative for H. pylori IgG antibody testing.

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Section: Discussionmentioning

confidence: 99%

Use of a dried plasma collection card for simplified diagnosis of Helicobacter pylori infection

Boes²,

1999

Aliment Pharmacol Ther

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Novel Diagnostic Tests to DetectHelicobacter pyloriInfection: A Pediatric Perspective

1999

Canadian Journal of Gastroenterology

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Helicobacter pylori is one of the most common bacterial infections in humans, and has been found in differing rates in persons of all age groups, nationalities and socioeconomic classes (1). Because the role of H pylori in human disease continues to be explored, there is an increased need for rapid, reliable and inexpensive diagnostic tests (2,3). The most commonly used standard tests are listed in Table 1. To date, the only test that can detect infection and disease activity is endoscopy with biopsy, which is invasive and expensive.Five recently developed tests that offer potential advantages because they are less invasive or permit easier acquisition of samples are assessed. These tests are whole blood, saliva and urine assays that measure systemic antibody response to H pylori, stool tests that measure H pylori antigens and string tests that use methodology less invasive than upper gastrointestinal endoscopy to recover H pylori organisms.The tests are compared with current standard tests and criteria for optimal tests. The criteria are non-or minimally invasive nature; high sensitivity, specificity, and positive and negative predictive values in diagnosing infection and assessing disease activity and effectiveness of therapy; convenience, including ease of acquisition of samples and rapid determination of results; and low cost. WHOLE BLOOD ANTIBODY TESTS Background:Whole blood antibody tests are based on the fact that systemic antibodies to H pylori are found in whole blood, making testing possible in a primary care setting (2-9). Whole blood tests have several important potential advantages compared with standard serological tests; the samples are easier to obtain, testing can be done in an office setting and the results can be available within 10 mins. In addition, the cost is projected to be less than that for the standard serological tests. CANADIAN HELICOBACTER CONSENSUS CONFERENCE ON PEDIATRIC ISSUES

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Evaluation of the salivary antibody response to Helicobacter pylori

Cited by 2 publications

References 0 publications

Use of a dried plasma collection card for simplified diagnosis of Helicobacter pylori infection

Use of a dried plasma collection card for simplified diagnosis of Helicobacter pylori infection

Novel Diagnostic Tests to DetectHelicobacter pyloriInfection: A Pediatric Perspective

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