Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method

Metgud, Rashmi; Khajuria, Nidhi; Mamta, .; Ramesh, Gayathri

doi:10.7860/jcdr/2016/11598.7161

Cited by 19 publications

(20 citation statements)

References 9 publications

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“…Eighty patients were studied among which 100% were secretors for A and O blood group and 95% were secretors for A and AB blood groups, respectively. 11 Al-Sihli AA conducted a study in 30 subjects to assess the correlation between blood groups and dental caries using absorption-inhibition method and results showed 63.3% were secretors and 36.7% were nonsecretors. Present study shower more secretor status.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Tejasvi

Bukkya²,

Rao

et al. 2021

Global Medical Genetics

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Objectives While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample. Materials and Methods A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method Results Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females. Conclusion Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.

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Section: Discussionmentioning

confidence: 99%

“…Eighty patients were studied among which 100% were secretors for A and O blood group and 95% were secretors for A and AB blood groups, respectively. 11 …”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Tejasvi

Bukkya²,

Rao

et al. 2021

Global Medical Genetics

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“…Blood group antigens are present on the surface of many human cells, including red blood cells, leukocytes, and epithelial and endothelial cells, and can be potentially targeted to facilitate association and modulation of host cellular responses (28–30). Approximately 80% of humans have blood group antigens present in their mucosal secretions (often referred to as “secretors”), allowing these glycans to be absorbed by cells for presentation on their surface (31). There may be a preference for binding human blood group antigens by N.…”

Section: Discussionmentioning

confidence: 99%

Glycointeractome of Neisseria gonorrhoeae: Identification of Host Glycans Targeted by the Gonococcus To Facilitate Adherence to Cervical and Urethral Epithelial Cells

Semchenko

Everest‐Dass

Jen

et al. 2019

mBio

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Neisseria gonorrhoeae is a significant threat to global health for which a vaccine and novel treatment options are urgently needed. Glycans expressed by human cells are commonly targeted by pathogens to facilitate interactions with the host, and thus characterization of these interactions can aid identification of bacterial receptors that can be exploited as vaccine and/or drug targets. Using glycan array analysis, we identified 247 specific interactions between N. gonorrhoeae and glycans representative of those found on human cells. Interactions included those with mannosylated, fucosylated, and sialylated glycans, glycosaminoglycans (GAGs), and glycans terminating with galactose (Gal), N-acetylgalactosamine (GalNAc), and N-acetylglucosamine (GlcNAc). By investigating the kinetics of interactions with selected glycans, we demonstrate that whole-cell N. gonorrhoeae has a high affinity for mannosylated glycans (dissociation constant [KD], 0.14 to 0.59 μM), which are expressed on the surface of cervical and urethral epithelial cells. Using chromatography coupled with mass spectrometric (MS) analysis, we identified potential mannose-binding proteins in N. gonorrhoeae. Pretreatment of cells with mannose-specific lectin (concanavalin A) or free mannose competitor (α-methyl-d-mannopyranoside) substantially reduced gonococcal adherence to epithelial cells. This suggests that N. gonorrhoeae targets mannosyl glycans to facilitate adherence to host cells and that mannosides or similar compounds have the potential to be used as a novel treatment option for N. gonorrhoeae. IMPORTANCE Multidrug-resistant strains of Neisseria gonorrhoeae are emerging worldwide, and novel treatment and prevention strategies are needed. Glycans are ubiquitously expressed by all human cells and can be specifically targeted by pathogens to facilitate association with host cells. Here we identify and characterize the N. gonorrhoeae host-glycan binding profile (glycointeractome), which revealed numerous interactions, including high-affinity binding to mannosyl glycans. We identify gonococcal potential mannose-binding proteins and show that N. gonorrhoeae uses mannosyl glycans expressed on the surface of cervical and urethral epithelia to facilitate adherence. Furthermore, a mannose-binding lectin or a mannoside compound was able to reduce this adherence. By characterizing the glycointeractome of N. gonorrhoeae, we were able to elucidate a novel mechanism used by this important pathogen to interact with human cells, and this interaction could be exploited to develop novel therapeutics to treat antibiotic-resistant gonorrhea.

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“…33 In India, various studies have given frequencies of nonsecretors at around 70 to 100%. 34,35 Surprisingly, of the remaining population, not all are found to be secretors, but some of them can be clubbed together to form another classification of weak secretors. 36 The larger part of Asian population reportedly show weak activity of fucosyltransferase-2 enzyme; hence, the marker Le b shows "dim" expression.…”

Section: Discussionmentioning

confidence: 99%

Studies on Single-nucleotide Polymorphisms in the FUT2 Gene and Their Association with Host Susceptibility to Rotavirus Infection of P[4] and P[8] Genotypes

Joshi¹,

Rane-Yadav²,

Jhurani³

et al. 2017

MGM Journal of Medical Sciences

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Rotavirus is a segmented double stranded ribonucleic acid virus with typical surface antigens, viral protein 7 and viral protein 4. Almost 5 million children all over world are reported to be infected with this virus. In vitro studies have shown binding of rhesus rotavirus surface protein with 'Sia' groups of a histo blood group antigens (HBGAs). In case of humans, rotavirus type A is more prevalent. The precise genomic diversity of ABO (H) and Lewis Blood Group System in various ethnic populations may provide plausible explanation for prevalence of specific P genotypes. Present study aims to identify role of Single Nucleotide Polymorphisms in the host FUT-2 gene in the host susceptibility to risk of rotavirus infection of P genotypes.The study indicates that null allele at certain loci of fucosyltransferase-2 (FUT2) gene, also known as secretor (Se) gene leads to lack of functionally active enzyme. This results in absence of α-1/2 fucosylated glycan and may protect the child against rotavirus infection of specific strain. FUT2 gene alleles at loci 428 (AA) and 302 (TT) are found to be associated with group A rotaviruses. Both these alleles were frequent in population under study. Presence of any of these allele in children of Indian origin leads to non-secretor phenotype and hence if exposed to P[4] and P[8] genotypes of rotavirus, can resist the infection.

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Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva among Southern Rajasthan Population Using Absorption Inhibition Method

Cited by 19 publications

References 9 publications

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

Glycointeractome of Neisseria gonorrhoeae: Identification of Host Glycans Targeted by the Gonococcus To Facilitate Adherence to Cervical and Urethral Epithelial Cells

Studies on Single-nucleotide Polymorphisms in the FUT2 Gene and Their Association with Host Susceptibility to Rotavirus Infection of P[4] and P[8] Genotypes

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