Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach

Álvarez, Julio; Pérez, Andrés; Bezos, Javier; Marques, Sérgio Corrêa; Grau, Anna; Sáez, José Luis; Mínguez, Olga; Juan, Lucía de; Domı́nguez, Lucas

doi:10.1016/j.vetmic.2011.07.034

Cited by 85 publications

(83 citation statements)

References 25 publications

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“…Latent class models with a Bayesian approach can then be used to estimate the diagnostic accuracy of the tests [18][19][20][21]. Bayesian approaches allow for the incorporation of prior knowledge.…”

Section: Discussionmentioning

confidence: 99%

“…To our knowledge only one paper uses a latent class approach in order to estimate sensitivity and specificity of the single intradermal comparative cervical skin test at different cut-offs (for zebu cattle in Chad) [29] and another paper uses a latent class approach to estimate test accuracies of the single intradermal test used in Spain [21]. Different cut-offs for skin thickness used in different countries clearly shows that there is no unambiguous interpretation of the test's results.…”

Section: Discussionmentioning

confidence: 99%

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The Accuracy of the Single Intradermal Comparative Skin Test for the Diagnosis of Bovine Tuberculosis-Estimated from a Systematic Literature Search

Hartnack

Torgerson²

2012

Mycobact Dis

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Background: Bovine Tuberculosis (bTN) elimination programs are based on test-and-slaughter strategies. No diagnostic test used in the control of Bovine Tuberculosis (bTB), a zoonotic disease, is a perfect gold standard having a diagnostic sensitivity and specificity of 100%. Methods: To find relevant data to assess diagnostic accuracy of one variant of the tuberculin test, the Single Intradermal Comparative Cervical Tuberculin Test (SICCT) used in the diagnosis of bTB a systematic literature search was performed. To estimate diagnostic test accuracies of the SICCT, a Bayesian latent class analysis has been undertaken. Results: Only one eligible study was found. Assuming bacterial isolation has a specificity of 100%, specificity of gross lesion detection and histopathology were estimated as 90% and 97% respectively. Estimates of the sensitivity of the Single Intradermal Comparative Cervical Tuberculin Test (SICCT), gross lesion detection, histopathology and bacteriological isolation were 80%, 89%, 93% and 98% respectively. The design of this study precluded estimating the SICCT specificity, although official UK surveillance data indicates specificity greater than 99.67%. Conclusion: Studies to estimate the diagnostic performance of key tests for bTB are lacking, yet essential for an informed debate in regard to bTB public health policy.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The Accuracy of the Single Intradermal Comparative Skin Test for the Diagnosis of Bovine Tuberculosis-Estimated from a Systematic Literature Search

Hartnack

Torgerson²

2012

Mycobact Dis

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“…Studies using this method are increasingly published, for cattle [126,179] as well as as for wildlife [81]. A disadvantage of Bayesian modeling is the use of prior assumptions, including independence of different tests, which complicates analyses of diagnostic tests that are based on the same stage of the immune response (i.e.…”

Section: Gold Standard Versus Latent Class Modelsmentioning

confidence: 99%

Facts and dilemmas in diagnosis of tuberculosis in wildlife

Maas¹,

Michel²,

Rutten³

2013

Comparative Immunology, Microbiology and Infectious Diseases

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Mycobacterium bovis, causing bovine tuberculosis (BTB), has been recognized as a global threat at the wildlife-livestock-human interface, a clear "One Health" issue. Several wildlife species have been identified as maintenance hosts. Spillover of infection from these species to livestock or other wildlife species may have economic and conservation implications and infection of humans causes public health concerns, especially in developing countries. Most BTB management strategies rely on BTB testing, which can be performed for a range of purposes, from disease surveillance to diagnosing individual infected animals. New diagnostic assays are being developed for selected wildlife species. This review investigates the most frequent objectives and associated requirements for testing wildlife for tuberculosis at the level of individual animals as well as small and large populations. By aligning those with the available (immunological) ante mortem diagnostic assays, the 2 practical challenges and limitations wildlife managers and researchers are currently faced with are highlighted.

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“…In developed countries, few infections show symptoms and infections are mostly diagnosed by routine tests. Tuberculosis is usually diagnosed in the field with tuberculin skin test [7,8] . Sputum and other body fluids may be collected for microbiological examination, microscopic demonstration of acid fast bacilli, isolation of mycobacteria on selective culture media and identification by biochemical tests.…”

Section: Introductionmentioning

confidence: 99%

Sahada Görülen Sığır Tüberkülozunun Tanısında Kullanılan Tuberculin Skin Test, IFN-γ Assay, Gerçek Zamanlı PCR ve Lateral Flow Rapid Testlerinin Karşılaştırılması

Kaya¹,

Şimşek²,

Yıldırım³

et al. 2015

Kafkas Univ Vet Fak Derg

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Bovine tuberculosis is an important zoonotic disease transmitted by direct contact, respiratory pathway, ingestion of unpasteurised milk and milk product, raw or undercooked meat. Tuberculosis can be difficult to diagnose based only on the clinical signs. Tuberculosis is usually diagnosed in the field with the tuberculin skin test. Sputum and other body fluids may be collected for microbiological examination. Polymerase chain reaction (PCR) methods have also been described. Diagnostic blood tests include the lymphocyte proliferation assay, the interferon gamma (IFN-γ) assay, and enzyme-linked immunosorbent assays (ELISA). In this study a total of 50 animals were tested by using tuberculin skin test (TST), lateral flow rapid test, IFN-γ assay and real time PCR. The animals were selected randomly among 178 cattle in dairy farms with the aged between 3-5 years and suspected of having tuberculosis. Forty five cattle were positive out of 50 for TST while 31 for reactive by the IFN-γ assay and 28 for rapid test and 9 for real time PCR. The purpose behind such variable as age was to compare sensitivity of tuberculin skin test, the IFN-γ assay and TB lateral flow rapid test and real time PCR examination for the diagnosis of field outbreaks of bovine tuberculosis in Turkey. Keywords: IFN-γ assay, Real time PCR, TB lateral flow rapid test, Tuberculin skin test, Tuberculosis Sahada Görülen Sığır Tüberkülozunun Tanısında Kullanılan Tuberculin Skin Test, IFN-γ Assay, Gerçek Zamanlı PCR ve Lateral Flow Rapid Testlerinin KarşılaştırılmasıÖzet Sığır tüberkülozu; direkt temas, solunum yolu, pastörize edilmemiş süt ve süt ürünleri, çiğ ya da az pişmiş etlerin alınmasıyla insanlara da bulaşabilen önemli bir zoonoz hastalıktır. Yalnızca klinik bulgulara bakarak tüberkülozu teşhis etmek zor olabilmektedir. Genellikle tüberkülozun tanısı sahada tüberkülin testiyle konulmaktadır. Mikrobiyolojik muayene amacıyla kraşe ve diğer vücut sıvıları temin edilmektedir. Polymerase chain reaction (PCR) yöntemleri de kullanılabilmektedir. Tanı amaçlı kan testleri arasında lenfosit proliferasyon testi, interferon gamma (IFN-γ) testi ve enzyme-linked immunosorbent assay (ELISA) bulunmaktadır. Bu çalışmada 50 hayvan tuberculin skin test (TST), lateral flow rapid test, IFN-γ assay ve gerçek zamanlı PCR kullanılarak test edilmiştir. Hayvanlar 3-5 yaş arasındaki tüberküloz şüpheli 178 sütçü sığır arasından rastgele seçilmiştir. TST sonuçlarına göre 50 sığırdan 45'i pozitifken, IFN-γ testine göre 31'i pozitif bulunmuştur. TB lateral flow rapid testte 28 pozitif, gerçek zamanlı PCR testinde ise 9 pozitif sonuç bulunmuştur. Çalışmamızda farklı testlerin uygulanmasının amacı tüberkülin skin test, IFN-γ assay, TB lateral flow rapid test ve gerçek zamanlı PCR testinin Türkiye'de karşılaşılan sığır tüberkülozundaki duyarlılıklarının karşılaştırılmasıdır.

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Evaluation of the sensitivity and specificity of bovine tuberculosis diagnostic tests in naturally infected cattle herds using a Bayesian approach

Cited by 85 publications

References 25 publications

The Accuracy of the Single Intradermal Comparative Skin Test for the Diagnosis of Bovine Tuberculosis-Estimated from a Systematic Literature Search

The Accuracy of the Single Intradermal Comparative Skin Test for the Diagnosis of Bovine Tuberculosis-Estimated from a Systematic Literature Search

Facts and dilemmas in diagnosis of tuberculosis in wildlife

Sahada Görülen Sığır Tüberkülozunun Tanısında Kullanılan Tuberculin Skin Test, IFN-γ Assay, Gerçek Zamanlı PCR ve Lateral Flow Rapid Testlerinin Karşılaştırılması

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