Evaluation of the Uncertainty of a Quantitative Approach to Drug Metabolites in the Absence of Authentic Standards

Purity estimation of seized stimulant-type new psychoactive substances without reference standards by nitrogen chemiluminescence detection combined with GC-APCI-QTOFMS

“…Apart from NMR spectroscopy [11], there has been limited research activity within forensic sciences concerning identification combined with universal quantification methods in the absence of primary reference standards. However, with MS and UV -based methods, relying only on secondary standards is risky due to compound-specific ion responses [9,21]. Importantly, there are no established guidelines for analysis in such a scenario where the primary reference standards are missing, however, we think that implementation of a statistical uncertainty model could be beneficial in such circumstances [21].…”

“…However, with MS and UV -based methods, relying only on secondary standards is risky due to compound-specific ion responses [9,21]. Importantly, there are no established guidelines for analysis in such a scenario where the primary reference standards are missing, however, we think that implementation of a statistical uncertainty model could be beneficial in such circumstances [21].…”