Evaluation of the VITAL (bioMérieux) automated blood culture system using blind subculture

Giménez, Montserrat; Prat, Cristina; Vallès, Xavier; Matas, Lourdes; Arnal, J; Ausina, V.

doi:10.1046/j.1469-0691.2002.00417.x

Cited by 6 publications

(4 citation statements)

References 26 publications

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“…At the end of the first week, the detection rates increased to 73.7%, 94.7%, and 47.4%, respectively, proving that the Bactec 9120 was significantly faster than the two other blood culture systems (P Ͻ 0.05). The delayed detection of Brucella by the Vital system was confirmed in two later studies in which the average detection occurred after incubation times of 119.7 and 211.7 h (84,85).…”

Section: (Vi) Is the Traditional Recommendation Of Prolonged Incubatimentioning

confidence: 65%

Laboratory Diagnosis of Human Brucellosis

2019

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SUMMARY The clinical presentation of brucellosis in humans is variable and unspecific, and thus, laboratory corroboration of the diagnosis is essential for the patient’s proper treatment. The diagnosis of brucellar infections can be made by culture, serological tests, and nucleic acid amplification assays. Modern automated blood culture systems enable detection of acute cases of brucellosis within the routine 5- to 7-day incubation protocol employed in clinical microbiology laboratories, although a longer incubation and performance of blind subcultures may be needed for protracted cases. Serological tests, though they lack specificity and provide results that may be difficult to interpret in individuals repeatedly exposed to Brucella organisms, nevertheless remain a diagnostic cornerstone in resource-poor countries. Nucleic acid amplification assays combine exquisite sensitivity, specificity, and safety and enable rapid diagnosis of the disease. However, long-term persistence of positive molecular test results in patients that have apparently fully recovered is common and has unclear clinical significance and therapeutic implications. Therefore, as long as there are no sufficiently validated commercial tests or studies that demonstrate an adequate interlaboratory reproducibility of the different homemade PCR assays, cultures and serological methods will remain the primary tools for the diagnosis and posttherapeutic follow-up of human brucellosis.

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Section: (Vi) Is the Traditional Recommendation Of Prolonged Incubatimentioning

confidence: 65%

Laboratory Diagnosis of Human Brucellosis

2019

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“…GBS isolation by culture is considered the reference standard. Automated culture methods yield higher detection rates compared to manual culture methods 105 , 308 ; minimizing time between collection and inoculation of blood culture bottles, using pediatric bottles for young infants, and maximizing blood volumes are important for optimal results 105 . For GBS meningitis, in addition to positive CSF culture, case definitions have included detection of GBS antigens in CSF ( e.g.…”

Section: Considerations About Options To Generate Pivotal Licensure Dmentioning

confidence: 99%

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries

et al. 2016

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Globally, group B Streptococcus (GBS) remains the leading cause of sepsis and meningitis in young infants, with its greatest burden in the first 90 days of life. Intrapartum antibiotic prophylaxis (IAP) for women at risk of transmitting GBS to their newborns has been effective in reducing, but not eliminating, the young infant GBS disease burden in many high income countries. However, identification of women at risk and administration of IAP is very difficult in many low and middle income country (LMIC) settings, and is not possible for home deliveries. Immunization of pregnant women with a GBS vaccine represents an alternate pathway to protecting newborns from GBS disease, through the transplacental antibody transfer to the fetus in utero. This approach to prevent GBS disease in young infants is currently under development, and is approaching late stage clinical evaluation. This manuscript includes a review of the natural history of the disease, global disease burden estimates, diagnosis and existing control options in different settings, the biological rationale for a vaccine including previous supportive studies, analysis of current candidates in development, possible correlates of protection and current status of immunogenicity assays. Future potential vaccine development pathways to licensure and use in LMICs, trial design and implementation options are discussed, with the objective to provide a basis for reflection, rather than recommendations.

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“…When the incubation was extended to 7 days, the results were 73.7%, 94.7%, and 47.4%, respectively, indicating that the BACTEC system was significantly faster than the comparators (P<0.05). The delayed detection of brucellae by the VITAL system was confirmed in two later studies in which the time-to-positivity for members of the genus was 119.7 and 211.7 hours [49,50].…”

Section: Comparative Studies Involving Fully Automated Blood Culture mentioning

confidence: 72%

Blood Cultures for the Diagnosis of Human Brucellosis

Yagupsky¹

2015

Updates on Brucellosis

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Brucellosis represents a serious health threat to human populations living in areas endemic for the disease. The clinical manifestations of brucellosis are protean and nonspecific, and laboratory confirmation of the diagnosis is crucial for an adequate management of the patient and implementation of infection control measures aimed to control the disease in affected herds. Although brucellosis can be confirmed by serologic tests and nucleic acid amplification assays, culture detection of circulating Brucella organisms remains a diagnostic cornerstone. Traditionally, prolonged incubation of media and performance of blind subcultures of negative blood culture vials have been recommended to maximize isolation of the organism. In recent years, modern automated blood culture systems have revolutionized the diagnosis of human brucellosis by improving sensitivity and enabling detection of brucellae within the routine one-week incubation protocol followed in most Clinical Microbiology laboratories. Development of molecular techniques and mass-spectrometry technology have also shortened the time needed to identify members of the genus, whereas use of biological safety cabinets considerably reduce the risks of contagion to laboratory personnel.

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Evaluation of the VITAL (bioMérieux) automated blood culture system using blind subculture

Cited by 6 publications

References 26 publications

Laboratory Diagnosis of Human Brucellosis

Laboratory Diagnosis of Human Brucellosis

Group B Streptococcus vaccine development: present status and future considerations, with emphasis on perspectives for low and middle income countries

Blood Cultures for the Diagnosis of Human Brucellosis

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