Evaluation of the water genotoxicity from Santos Estuary (Brazil) in relation to the sediment contamination and effluent discharges

Umbuzeiro, Gisela de Aragão; Kummrow, Fábio; Roubicek, Deborah A.; Tominaga, Maria Yumiko

doi:10.1016/j.envint.2005.08.030

Cited by 25 publications

(12 citation statements)

References 18 publications

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“…The implementation of programs and models for biomonitoring in countries like Brazil, with continental dimensions and very large geographic and biological diversity, should consider the standardization of methods based on each species and ecosystem [2][3][4][5][6]. It is possible to develop mathematical modeling methodologies on the behavior of fish in situations of stress caused by impacts of anthropic origin which can be applied in the most diverse regions.…”

Section: Introductionmentioning

confidence: 99%

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Neta

Torres

Abreu-Silva

2012

Appl Biochem Biotechnol

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Biomarkers based on specific enzyme activities and histological alterations are useful tools for evaluating toxicological effects of xenobiotics in wild fish. In this work, an experimental system of biomarkers with enzyme glutathione S-transferase (GST) and branchial lesions in catfish (Sciades herzbergii) was mathematically modeled. The fish were collected along known pollution gradients (S1) and from areas regarded relatively free of anthropogenic input (S2) in São Marcos' Bay, Brazil. GST was measured spectrophotometrically, and branchial lesions were examined by light microscope. The databases from this analysis were compiled, and non-linear models were used to analyze the dependence of the enzyme activity on the areas of sampling and on selected biological parameters of the fish. Fish weight, length, and somatic indices (gonadosomatic index) were significant in the model of GST activity only in A2. Brachial lesions were significant in the model of GST activity only in A1. The obtained model indicates that when the GST ceases to act, serious branchial lesions are observed in the fish of the contaminated regions.

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Section: Introductionmentioning

confidence: 99%

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Neta

Torres

Abreu-Silva

2012

Appl Biochem Biotechnol

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“…Most surveys of PAH contamination in water bodies have been in conducted in North America and Europe, such as in USA [12][13][14], Canada [15], Germany [16][17][18][19], England [20], France [21], Greece [3] and other countries [22]. Information concerning water pollution with PAHs in South America has been reported for Brazil [23][24][25] and Chile [26]. The majority of these surveys worldwide were mainly focused on seawater (coastal of offshore) and estuarine water.…”

Section: Introductionmentioning

confidence: 99%

Persistent toxic substances in surface water of Todos Os Santos Bay, Brazil

Celino¹,

Corseuil²,

Fernandes³

et al. 2012

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Persistent toxic substances (PTSs) such as polycyclic aromatic hydrocarbons (PAHs) are of great concern due to their persistence, bioaccumulation and toxic effects. In this work, 14 PAHs included in the US Environmental Protection Agency's (EPA) priority pollutant list were analysed using gas chromatography-mass spectrometry (GC-MS). Surface water sampling was undertaken in Todos os Santos Bay, northern Brazil. Total PAH concentrations varied from 0.0029 to 0.1079 ng/L in surface waters (main rivers, tributaries, etc.) with mean value of 0.0344 ng/L. Such concentrations can be taken as background values for the studied region and show that PAHs are within or lower than levels reported in certain other areas. The PAH profiles were dominated by high molecular weight PAHs (four-and five-ring components) in surface water samples. It indicated that PAHs in surface water have its origin from oil or sewage contamination (petrogenic input). The collected data, based on indices among phenanthrene versus anthracene, showed that petrogenic input was predominant at almost all the stations investigated. Principal component analysis (PCA), using a correlation matrix, revealed the latent relationships among all the surface water stations investigated and confirmed our analytical results.

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“…Several studies have shown that the sediment of some areas of this channel contain high levels of mutagenic polycyclic aromatic hydrocarbons (PAHs) along with other tox-icants [CETESB, 2001;Nishigima et al, 2001;Medeiros and Bícego, 2004;Bícego et al, 2006;Umbuzeiro et al, 2006;Cesar et al, 2007;Martins et al, 2007]. High levels of mutagenic activity are also associated with the sediment [Roubicek, 2003] and water [Kummrow et al, 2006a,b] of the most contaminated sites.…”

Section: Introductionmentioning

confidence: 99%

Mutagenicity of blue rayon extracts of fish bile as a biomarker in a field study

Santos

Kummrow

Albergaria-Barbosa

et al. 2009

Environ and Mol Mutagen

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Blue rayon (BR) in combination with the Salmonella/microsome assay was used to evaluate the mutagenicity of fish bile samples. Specimens of Mugil curema from two sites were collected over a 1-year period. Piaçaguera channel contains high concentrations of total polycyclic aromatic hydrocarbons (PAHs) and other contaminants, while Bertioga channel was considered the reference sites in this study. Bile was extracted with BR and tested with TA98, TA100, and YG1041 strains with and without S9 in dose response experiments. PAH metabolite equivalents were analyzed using reverse-phase high performance liquid chromatography /fluorescence. Higher mutagenic responses were observed for the contaminated site; YG1041 with S9 was the most sensitive strain/condition. Mutagenicity ranged from 3,900 to 14,000 rev./mg at the contaminated site and from 1,200 to 2,500 rev./mg of BR at the reference site. The responses of YG1041 were much higher in comparison with the TA98 indicating the presence of polycyclic compounds from the aromatic amine class that cause frameshift mutation. TA100 showed a positive mutagenic response that was enhanced following S9 treatment at both sites suggesting the presence of polycyclic compounds that require metabolic activation. benzo(a)pyrene, naphthalene, and phenanthrene metabolite equivalents were also higher in the bile of fish collected at the contaminated site. It was not possible to correlate the PAH metabolite quantities with the mutagenic potency. Thus, a combination of the Salmonella/microsome assay with YG1041 with S9 from BR bile extract seems to be an acceptable biomarker for monitoring the exposure of fish to mutagenic polycyclic compounds.

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Evaluation of the water genotoxicity from Santos Estuary (Brazil) in relation to the sediment contamination and effluent discharges

Cited by 25 publications

References 18 publications

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Biomarkers in Catfish Sciades herzbergii (Teleostei: Ariidae) from Polluted and Non-polluted Areas (São Marcos’ Bay, Northeastern Brazil)

Persistent toxic substances in surface water of Todos Os Santos Bay, Brazil

Mutagenicity of blue rayon extracts of fish bile as a biomarker in a field study

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