1988
DOI: 10.1111/j.1423-0410.1988.tb01622.x
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Evaluation of Three Second‐Generation and Three Confirmatory Assays for Antibodies to Human Immunodeficiency Virus

Abstract: The second-generation enzyme immunoassays (EIAs) for antibodies against human immunodeficiency virus (HIV) from three manufacturers (Abbott, Organon, Wellcome) and three anti-HIV confirmatory tests, i.e. Western Blot (WB, Biotech, Dupont), radioimmunoprecipitation assay (RIPA, CLB) and a competitive immunoassay (CIA, Abbott) were evaluated on a panel of 6,488 serum samples, which had previously been used for the comparison of seven first-generation EIAs. For the present study the panel was expanded with sequen… Show more

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Cited by 21 publications
(4 citation statements)
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“…We believe that the WB assay in these cases of atypical anti-p248a® reactivity recognizes crossreacting antibodies against a common epitope or epitopes, which may be enhanced by the changes in structure of the viral proteins during the WB procedures, and therefore are hardly found with 125I-RIPA, which in our hands is as sensitive as WB in detecting true anti-p24gag antibodies [13,15]. This is supported by the specific inhibition of the reactivity with infected H9 cells.…”
Section: Donors With Anti-p24gassupporting
confidence: 62%
See 1 more Smart Citation
“…We believe that the WB assay in these cases of atypical anti-p248a® reactivity recognizes crossreacting antibodies against a common epitope or epitopes, which may be enhanced by the changes in structure of the viral proteins during the WB procedures, and therefore are hardly found with 125I-RIPA, which in our hands is as sensitive as WB in detecting true anti-p24gag antibodies [13,15]. This is supported by the specific inhibition of the reactivity with infected H9 cells.…”
Section: Donors With Anti-p24gassupporting
confidence: 62%
“…In HIV-1 WB by Biotech-Du Pont (WB-Dupont), 29 of these samples (17 only initially, and 12 repeatedly ELISA positive) showed anti-p24gag reactivity (once combined with antipl8gag and twice combined with anti-pr55gag) without reactivity to other (glyco-) proteins [13].…”
Section: Donorsmentioning
confidence: 99%
“…WB tests determine the protein to which the antibodies were developed. The tests are of higher specificity than ELISA [57][58][59].…”
Section: Conjugatementioning
confidence: 94%
“…W przypadku testów WB można określić, do którego białka zostały wytworzone przeciwciała. Testy te charakteryzują się wyższą swoistością niż testy ELISA [57][58][59].…”
Section: Ewolucja Metodyki Badań Diagnostycznych I Przeglądowychunclassified