EVALUATION OF TOTAL PHENOLIC, FLAVONOID CONTENT, ANTIOXIDANT AND IN VITRO ANTILITHOGENESIS ACTIVITIES OF CHIVES LEAF (Allium schoenoprasum, L.)

Abstract

Natural resources and traditional medicines are important assets that need to be explored, researched, developed and optimized for their use. One of the natural resources that can be used as medicinal ingredients is chives (Allium schoenoprasum, L.), especially the leaf. Chives leaf contained a lot of phytochemical compounds which is potential as an antioxidant and antilithogenesis. The objective of this research is to evaluate the antioxidant and antilithogenesis activities of chives leaf extract. Antioxidant activity was evaluated by using DPPH as a free radical scavenging method. Total phenol and total flavonoid content also determined by using Folin-Ciocalteu and aluminum chloride colorimetric assay. Antilithogenesis assay was measuring using atomic absorption spectrophotometry method by incubating the kidney stone using extracts of chives leaves at 37 o C for 6 hours. The measurement of calcium was using acetylene-air flow at 422.7 nm wavelength. From the data obtained, the highest antioxidant activity was found in ethyl acetate extract (IC50: 236.51±0.08 ppm). The highest phenolic content was found in the ethanolic extract (111.28±1.29 mg/g extract) while the total flavonoid was found in ethyl acetate extract (34.64±1.60 mg/g extract). While, antilithogenesis activity showed that the best result was using 2.5% (E5) concentration with 95.55% solubility of calcium which obtained from ethyl acetate extract.Based on the above results, it can be concluded that chives leaf extracts have a potential and promising as an antioxidant and antilithogenesis sources

EXTRACTION, CHARACTERIZATION AND INVITRO BIOACTIVITY EVALUATION OF ALKALOIDS, FLAVONOIDS, SAPONINS AND TANNINS OF Cassia alata, Thespesia populnea, Euphorbia hirta AND Wrightia tinctoria

“…5 Recent reports revealed that phenolic components like flavonoids possibly form a complex with the calcium and potassium oxalates which could provide a potential cure for treating kidney stones. 6 Alkaloids, on the other hand, mimics the neuro transmitters' as agonists or antagonists in binding to the molecular receptors and their usage dates back to more than 5000 years as hallucinogens and psychotropic drugs. 7 Tannins enriched plant extracts are mostly used by the traditional practitioners as astringents, diuretics, to treat diarrhoea, to reduce ulceration of tumor tissues of the stomach and duodenum.…”

TANNIN AND ANTIOXIDANT STATUS OF FERMENTED AND DRIED Sorghum bicolor

“…They have various biological activities such as antioxidant, anti-inflammatory, anti-allergic, anticarcinogenic, anti-mutagenic and modulatory effects on enzymatic activities. Flavonoids possess high antioxidant activities that can protect the body system against free radical and other diseases such as arteriosclerosis and diabetes 9,[26][27][28] .…”

PHYTOCHEMICAL SCREENING AND ANTIHYPERGLYCEMIC ACTIVITY OF ETHANOLIC EXTRACT OF Coriandrum sativum L. LEAF

“…About 300 g of the fine powder coriander leaf was extracted with 96% ethanol by using maceration for 5 days at room temperature.The ethanol extract of coriander leaf (EECL) solution part was evaporated by using rotary evaporator to get the crude extract. 6,7 Preliminary Phytochemical Screening The crude extract of EELC was screening by using the standard protocol to know the presence of phytochemical compounds. 8…”