Evaluation of toxicity of β-tethymustine, a new anticancer compound, in mice

Ghosh, Manju; Sadhu, Utpal; Bhattacharya, Sudin; Dutta, Sushanta; Bhattacharya, Bhupati; Sanyal, Utpal

doi:10.1016/s0304-3835(98)00384-x

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…The pathological changes caused by carcinogenesis and its inhibition by chemotherapy are expected to be reflected in the biochemical and histological parameters of the host system, particularly pertaining to the liver which is known to be the major organ affected in EAC bearing mice (Ghosh et al 1999). Hence liver was chosen as the organ of study for examination of the level of oxidative stress as well as histopathological nature.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of antitumor activity of stigmasterol, a constituent isolated from Bacopa monnieri Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Ghosh¹,

Maity

Singh

2011

Orient Pharm Exp Med

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Cancer remains as one of the most common cause of mortality worldwide. Cancer chemoprevention by natural products is well accepted nowadays. Phytoserols are natural products, showing anticancer activity, besides other activities. The purpose of this study was to examine the antitumor and antioxidant activity of stigmasterol, a phytosterol isolated from aerial parts of Bacopa monnieri Linn. against Ehrlich Ascites Carcinoma (EAC) in swiss albino mice. The effect of stigmasterol on the growth of transplantable murine tumor, life span of EAC bearing hosts, simultaneous alterations in the hematological profile, liver biochemical parameters (lipid peroxidation, antioxidant enzymes) and histopthological studies of liver were examined. Stigmasterol decreased tumor volume, packed cell volume and viable cell count, and increased mean survival time thereby increasing life span of EAC tumor bearing mice. Hematological profile reverted to near normal levels in stigmasterol treated mice. Stigmasterol decreased the levels of lipid peroxidation and increased the levels of glutathione, superoxide dismutase and catalase in the liver of EAC bearing mice. Histopathological study of liver tissues showed that stigmasterol had significant protective effect against EAC bearing mice, which corroborates the above findings. Lactate dehydrogenase (LDH) activity in ascitic fluid increased while membrane microviscosity of the EAC cells decreased upon stigmasterol treatment indicating its effect in the membrane integrity functions. The antitumor activity of stigmasterol might be mediated through the activation of protein phosphatase 2A by ceramide causing apoptosis, as is shown by structurally similar phytosterol.

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Section: Resultsmentioning

confidence: 99%

Evaluation of antitumor activity of stigmasterol, a constituent isolated from Bacopa monnieri Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Ghosh¹,

Maity

Singh

2011

Orient Pharm Exp Med

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“…Vários autores utilizam a determinação das AgNORs como uma estratégia indicadora da proliferação celular (Silva et al, 2004;Silva et al, 2006;Romão-Correa et al, 2005 (Ghosh et al, 1999). A mielossupressão é a principal e também a mais freqüente complicação na quimioterapia do câncer (Mazumder et al, 2005).…”

Section: Resultsunclassified

Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae) sobre o tumor de Erlich ascítico no camundongo

Junior¹,

Souza-Fagundes

Cassali³

et al. 2006

Arq. Bras. Med. Vet. Zootec.

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Efeito do miriadenolídeo isolado de RESUMOEstudou-se atividade antineoplásica de um produto natural isolado de Alomia myriadenia (miriadenolídeo) no modelo do tumor de Ehrlich em camundongos. Dezoito fêmeas de camundongo Swiss foram inoculadas com 2x10 7 células viáveis de tumor de Ehrlich via intraperitoneal (0,3ml) e posteriormente distribuídas aleatoriamente em três grupos que receberam: grupo I (controle) -0,3ml de solução de Hanks; grupo II31µg/kg de miriadenolídeo; e grupo III -139µg/kg de miriadenolídeo. No oitavo dia de experimento, foram realizados exames hematológicos e perfil protéico sérico eletroforético. Coletou-se todo o líquido ascítico para avaliação do volume, aparência, pH, contagem de células viáveis e inviáveis, realização de esfregaços para contagem de células claras e escuras, leucócitos e avaliação das regiões organizadoras de nucléolos argentafins (AgNORs). Foram realizados exames macro e microscópicos do baço, fígado e rins e aspirado o conteúdo da medula óssea dos fêmures direito e esquerdo de cada animal para avaliação da relação mielóide:eritróide. Não houve diferença significativa no volume, pH, contagem de células viáveis e inviáveis entre os três grupos estudados, observando-se valores de 17,6 x 10 4 células tumorais viáveis no grupo III, 27,7 x 10 4 no grupo II e 21,1 x 10 4 no grupo I. As AgNORs apresentaram-se pequenas, com distribuição difusa e incontáveis no grupo I, e em menor quantidade no grupo III. Os animais do grupo III apresentaram a menor concentração protéica total sérica (4,7g/dl) (P<0,05) quando comparados com os do grupo II (5,3g/dl) e do grupo I (5,1g/dl). Os valores de albumina foram semelhantes nos três grupos (2,6g/dl), e as globulinas totais foram maiores (P<0,05) no grupo II (2,71g/dl) quando comparadas com os valores médios do grupo III (2,11g/dl) e semelhantes ao grupo I (2,43g/dl). Não houve diferença entre alfa e beta globulinas entre os três grupos estudados, porém as gamaglobulinas foram maiores (P<0,05) no grupo II (1,72g/dl) quando comparadas com as do grupo III (1,13g/dl). Com relação ao eritrograma e leucograma, não houve diferença significativa entre os grupos tratados. A relação mielóide:eritróide foi maior (P<0,05) no grupo III (1,40) quando comparada com a relação nos grupos I (0,92) e II (0,61). A contagem de reticulócitos também foi maior (P<0,05) (11,2) no grupo III, quando comparada com as dos grupos I (4,3) e II (3,6). Em todos os grupos, observou-se degeneração hepática. II (3.6). In all groups, hepatic degeneration was observed.

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“…The number of lymphocytes undergoes massive depletion following tumor transplantation and growth inside the host organism [24,25] . In Sarcoma 180-bearing mice model, evident reduction in splenic lymphocytes was observed, reinforcing the notion that tumor development inside the host resulted in the impairment of normal lymphocytes and the reduction of proliferative potential, which was in accordance with those previously reported [26,15] .…”

Section: Discussionmentioning

confidence: 99%

Aqueous Extract of Inonotus bliquus (Fr.) Pilat (Hymenochaetaceae) Significantly Inhibits the Growth of Sarcoma 180 by Inducing Apoptosis

Chen¹,

Zheng²,

Gao³

et al. 2007

American J. of Pharmacology and Toxicology

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Inonotus obliquus (Fr.) Pilat (Hymenochaetaceae) has been widely used as a folk medicine to treat various cancers in Russia, Poland and most of Baltic countries for literally more than four centuries. However, in spite of its therapeutic significance for cancers, this fungus has not been studied systematically for the mechanisms of antitumor activity. In this study, we report the effects of aqueous extracts of I. obliquus (AEI) on inducing apoptosis of Sarcoma 180. AEI prepared from the sclerotium of I. obliquus was either administered to Sarcoma 180-bearing mice or supplemented into the culture of Sarcoma 180 cells. Oral administration of AEI significantly inhibited Sarcoma 180-induced reduction of splenic lymphocytes and proliferation potentials and elicited over-expression of bax gene in Sarcoma 180 cells, leading to the regression of Sarcoma 180 in mice. Supplementation of AEI into the culture of Sarcoma 180 cells resulted in a significant increase in the number of cells in G0-G1 phase and triggered remarkable increment in programmed death (apoptosis) of Sarcoma 180 cells. In addition, AEI showed stimulatory effect on TNF-α release by mice peritoneal macrophages. These results indicated that the antitumor activity of AEI was mediated by protecting splenic lymphocytes from tumor-induced apoptosis and by triggering apoptosis of tumor cells.

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Evaluation of toxicity of β-tethymustine, a new anticancer compound, in mice

Cited by 8 publications

References 9 publications

Evaluation of antitumor activity of stigmasterol, a constituent isolated from Bacopa monnieri Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Evaluation of antitumor activity of stigmasterol, a constituent isolated from Bacopa monnieri Linn aerial parts against Ehrlich Ascites Carcinoma in mice

Efeito do miriadenolídeo isolado de Alomia myriadenia (Asteraceae) sobre o tumor de Erlich ascítico no camundongo

Aqueous Extract of Inonotus bliquus (Fr.) Pilat (Hymenochaetaceae) Significantly Inhibits the Growth of Sarcoma 180 by Inducing Apoptosis

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