Evaluation of two methods to measure hemoglobin concentration among women with genetic hemoglobin disorders in Cambodia: A method-comparison study

Accuracy of Capillary Hemoglobin Measurements for the Detection of Anemia among U.S. Low-Income Toddlers and Pregnant Women

“…In previous studies, the accuracy of Hb measurements of capillary blood conducted with portable hemoglobinometers (compared with Hb measurements of venous blood conducted with a reference method such as Coulter Counter (Beckman Coulter, Indianapolis, IN, USA), a device used to measure Hb using the cyanmethohemoglobin spectrophotometry method), varied within and across populations [7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26]. Furthermore, only one of these studies included toddlers (children aged 12–35 months), and the authors of that study did not provide estimates of the accuracy of capillary sampling for the detection of anemia specifically among toddlers [19].…”

Variation in haemoglobin measurement across different HemoCue devices and device operators in rural Cambodia

“…Factors such as humidity2 and blood collection technique3 4 have been reported to influence Hb concentration with the use of a haemoglobinometer in field settings. For example, milking the finger to stimulate blood flow can push extra interstitial fluid into the capillary sample, causing an underestimation of Hb concentration 5.…”

A method comparison study between two hemoglobinometer models (Hemocue Hb 301 and Hb 201+) to measure hemoglobin concentrations and estimate anemia prevalence among women in Preah Vihear, Cambodia

“…A bias in Hb of 2 g/L can be clinically significant; however, it depends on the mean Hb concentration of the population and on the distribution of Hb concentration of the population. If Hb is normally distributed and the mean is close to the cutoff for anemia, then a 2 g/L shift in Hb concentration (bias) could result in substantial differences in anemia prevalence rates . This could have important implications when comparing prevalence rates across surveys or populations, or when assessing anemia prevalence to plan appropriate nutrition programs or policies.…”

“…Anemia prevalence rates are used to guide national nutrition and public health policies. Automated hematology analyzers are commonly used in laboratories to determine Hb concentration and are referred to as the new gold standard for Hb measurement , due to their advantages of standardized quality control processes and calibration methods . Automated hematology analyzers require fresh whole blood.…”