Evaluation of two rapid immunochromatographic tests for diagnosis of brucellosis infection in cattle

Quintero, Anitza Fragas; Herrera, Dervel Felipe Díaz; Alfonso, Dayamí Martín; Santana, Yanelis Cruz; Torres, Raisa Betancourt; Tamayo, Lucy Montano

doi:10.4314/ovj.v8i3.2

Cited by 3 publications

(3 citation statements)

References 14 publications

(32 reference statements)

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“…Accurate and rapid diagnosis of re-emerging zoonotic diseases is critical for their control and monitoring. In brucellosis, the early diagnosis of the infected animals is necessary for controlling and eradicating the disease [19]. Therefore, the availability of a rapid and simple test that does not require highly sophisticated equipment and laboratories and that can be used as a screening field test is of great importance [20].…”

Section: Discussionmentioning

confidence: 99%

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

et al. 2022

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Background and Aim: Brucellosis is a zoonotic disease with a worldwide distribution. It has a serious impact on the health of humans and animals, along with a negative impact on the economy. This study aimed to prepare and evaluate the diagnostic performance of a lateral flow immunochromatographic test (LFIT) nanogold diagnostic kit for detecting brucellosis in sheep. Materials and Methods: A rapidly developed LFIT, in which lipopolysaccharide conjugates with nanogold molecules, was placed on the conjugate pad. One hundred ovine serum samples were tested to detect Brucella antibodies (Ab) using the prepared lateral flow immunochromatography assay (LFA) kit and Rose Bengal test. The evaluation of specificity, sensitivity, and accuracy for LFIT and Rose Bengal plate test was conducted using the P04310-10 IDEXX brucellosis ovine/ caprine Ab enzyme-linked immunosorbent assay (ELISA) test (gold standard). Results: The lower amount of Brucella Ab in the ovine serum samples was detected and was 1.58 S/P ratio ELISA titer/100 μL using LFIT and with Rose Bengal to detect 1.86 S/P ratio ELISA. The results showed that the developed LFIT had high specificity with no cross-reactivity with other tested bacteria. The calculated sensitivity, specificity, and accuracy of LFIT and Rose Bengal test using the P04310-10 IDEXX brucellosis ovine/caprine Ab ELISA test (gold standard) were 74% and 89%, 81% and 59%, and 76.9% and 66%, respectively. Conclusion: The present results showed interesting results implying that the LFIA strip test could be used as a substantial diagnostic tool for field screening ovine Brucella as an essential step in the control of brucellosis. However, further studies for the validation of the present findings are necessary.

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Section: Discussionmentioning

confidence: 99%

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

et al. 2022

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“…Bovine brucellosis is a zoonotic disease caused by a gram-negative bacterium called Brucella abortus and presents a worldwide distribution [1,2,3] and generates great economic losses in livestock bovine production [4,5] due to abortions, reduction in calf production, increase in calving intervals from 11.5 to 20 months, reduction in milk and meat production by 25% infertility and sterility in animals [6,7]. The presence of this agent in the cattle herd has economic implications as barriers to importing markets and losses such as loss of milk production and carcass condemnations of slaughtered cattle [8,9].…”

Section: Introductionmentioning

confidence: 99%

Sensitivity of acidified tamponed antigens in quality control test validation methodologies

Dias

Galvão

Faria

et al. 2022

Preprint

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Brucellosis is responsible for great economic losses and because it is a zoonotic, chronic, infectious, contagious disease, there is a great interest in its eradication in the cattle rearing in Brazil. One of the most effective measures to combat it is the vaccination of the animals and the diagnosis of positive animals, which contributes to the sanitation of the rearing. For this, it is necessary to use a practical, accessible, rapid and sensitive diagnostic methodology. The objective of this study was to evaluate the uncertainty of the test of relative activity (sensitivity) for acidified tamponed antigen (ATA). This antigen is used for the diagnosis of Brucellosis caused by smooth strains of Brucella ssp. Thus, we verified the efficacy of some parameters such as selectivity, precision (limit of detection and intermediate accuracy) and reproducibility. In the analyzes performed in the tests, the performances of standard antigens were considered. It was verified that the acidified tamponed antigen quality control method showed adequate selectivity, limit of detection, intermediate accuracy, repeatability and reproducibility in the verification of sensitivity tests for brucellosis antigens in the LFDA-MG. No difference was verified in the results of the various replicates. The ATA test, using a stable and sensitive antigen that shows high agreement with the confirmatory test, has a high potential to contribute to the identification of positive animals and sanitation of the herd, as well as to reduce the frequency of brucellosis and its subsequent eradication of the bovine herd.

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“…It is a highly contagious bacterial disease, which is considered the second most important zoonotic disease after rabies and has gained prominence over years since its discovery in island of Malta (Hashem et al, 2020). It has a great economic and social importance because of the huge losses it can cause in the livestock industry (Quintero et al, 2018). This disease is caused by four to six members of the genus Brucella.…”

Section: Introductionmentioning

confidence: 99%

Clinicopathological and Immunological studies on brucellosis

Eldin

Abdou

Farid

et al. 2021

Benha Veterinary Medical Journal

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Our view refers to clarify the seroprevalence of brucellosis infection in dairy cows in Menoufiya Governorate and to evaluate the hematological, biochemical and immunological parameters changes in blood of infected cows compared to healthy group. Blood samples of 100 dairy cows (3-5) years from private farm and Menoufiya abattoir were screened for Brucella infection using (BAPAT) test and groups, the first group consistent of ( 25) samples which serologically positive to brucella and the second group consistent of (75) samples which brucella negative. Hematological analysis revealed normocytic anemia and Lymphopenia. Biochemical analysis of brucella positive serum infected cows when compared with negative control revealed significant elevation in (AST), (ALT), (ALP) and (GGT) activity in addition to non-significant increases in creatinine level, however a significant decrease in serum urea in diseased cows was recorded. Total protein, α1 globulin, β2 globulin revealed significant decrease while non-significant change was observed in α2, β1 and γ globulin in infected group. Immunologically IL-1 β and IL-10 showed significant elevation in infected group when compared with negative control group. Brucella infectionBuffered acidified plate agglutination test.

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Evaluation of two rapid immunochromatographic tests for diagnosis of brucellosis infection in cattle

Cited by 3 publications

References 14 publications

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

Preparation and evaluation of a lateral flow immunochromatographic nanogold diagnostic kit for brucellosis in sheep

Sensitivity of acidified tamponed antigens in quality control test validation methodologies

Clinicopathological and Immunological studies on brucellosis

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