Evaluation of usage of silicon-doped hydroxyapatite ceramics for treatment of fragmented bone fractures in dogs

Rublenko, M.; Chemerovskiy, V. A.; Andriiets, Volodymyr; Ulyanchich, N. V.; Kolomiets, V. V.; Koryak, A. S.

doi:10.32718/nvlvet9905

Cited by 5 publications

(10 citation statements)

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“…E-mail: tatyana.todosyuk@gmail.com Постановка проблеми та аналіз останніх досліджень і публікацій. Серед хірургічної патології у тварин значну частку займають різноманітні травматичні ушкодження скелета, наслідком яких є переломи кісток [1][2][3][4][5][6][7][8]. Травми опорно-рухового апарату мають складний патогенез, спричиняються багатьма безпосередніми і сприяючими чинниками, що в результаті призводить до довготривалого періоду відновлення і, як наслідок, значних матеріальних та моральних витрат власників [9,10].…”

Section: білоцерківський національний аграрний університетunclassified

Clinical-radiological, hematological and biochemical assessment of bone replacement with germanium-doped calcium-phosphate ceramics for fragment fractures of tubular bones in dogs

Todosiuk,

Rublenko

2023

Nauk. vìsn. vet. med.

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Abstract. Among surgical pathology in animals, asignificant share is occupied by various traumatic injuries of the skeleton, the consequence of which are bonefractures. The results of X-ray and biochemical indicators of blood for bone fragment fractures in dogs arepresented.The purpose of the work is to clinically, radiologically, and pathochemically justify bone replacementwith germanium-doped calcium-phosphate ceramicsfor bone fragment fractures in dogs.In the injured animals of the experimental group(n=10), bone defects were replaced with germanium-doped calcium-phosphate ceramics (ГТлГег-700),in the control group (n=10) with unalloyed ceramics(ГТг-700). The activity of total alkaline phosphatase (ALP)and its bone isoenzyme (KiLP), tartrate-resistant acidphosphatase (TrKF) was determined in blood serum.Measurements were made with a Stat Fax 4500 spectrophotometer.On the 60th day, in the animals of the experimentalgroup, the filling of the bone defect with regeneratedmaterial of high X-ray density was noted, with the absence of periosteal reaction, which indicated the complete consolidation of the fracture and was the reasonfor the removal of the means of fixation. In the control,the area of the bone tissue defect that was not filledwith regenerate was visualized.The activity of tartrate-resistant acid phosphatase(TRP) in both groups tended to increase. On the 14th day,the animals of the experimental group had a peak activity of TrKf (33.8±0.31), which was 1.3 times (p<0.001)higher than the level in the control group and 1.5 times(p<0.001) of clinically healthy animals, respectively. Onthe 30th day of research, a 1.2-fold (p<0.001) decreasein the level of TrCF was noted in the experimental groupcompared to the control group, in which the indicatorreached peak values at 34.9±0.25 units/l.The maximum activity of the bone isoenzyme alkaline phosphatase (KiLF) in the experimental groupwas observed on the 14th day of reparative osteogenesis and was 41.7±0.43 units/l, which is 1.2 times(p<0.001) higher than the indicator of the controlgroup. On the other hand, in the last group, the peakactivity of the bone isozyme was noted only on the30th day (40.9±0.48 units/l), while the indicator of theexperimental animals decreased dynamically duringthis period.The use of calcium-phosphate ceramics for fragment fractures of the bones of the tibia in dogs helpsto reduce the intensity of the inflammatory-resorptivestage of reparative osteogenesis and accelerates consolidation by 1.4 times. The dynamics of biochemicalmarkers of bone metabolism testifies to the optimizedcourse of reparative osteogenesis during osteoreplacement of bone defects with germanium-doped calcium-phosphate ceramics. Key words: bioceramics, germanium, total alkaline phosphatase, bone isoenzyme of alkaline phosphatase, tartrate-resistant acid phosphatase, bonetissue.

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Section: білоцерківський національний аграрний університетunclassified

Clinical-radiological, hematological and biochemical assessment of bone replacement with germanium-doped calcium-phosphate ceramics for fragment fractures of tubular bones in dogs

Todosiuk,

Rublenko

2023

Nauk. vìsn. vet. med.

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“…Також встановлено (Shaganenko, 2012), що протягом двох тижнів після травми розвивається ендотеліальна дисфункція, із зниженням NO, що негативно впливає на формування судинної сітки у місці перелому, подовжуючи репаративні процеси. Також нами встановлено (Rublenko et al, 2020), що заміщення кісткових дефектів за осколкових переломів трубчастих кісток у собак керамікою на основі гідроксиапатиту і β-трикальційфосфату, легованої кремнієм, репаративний остеогенез прискорюється в 1,5 раза за рахунок скорочення його запально-резорбтивної стадії та прискорення проліферативної, головним чином з боку ендоосту з ранньою мінералізацією кісткового регенерату.…”

Section: вступunclassified

“…В післяопераційний період проводили клінічні та рентгенологічні дослідження, які висвітлені у попередній статті (Rublenko et al, 2020).…”

Section: матеріал і методи дослідженьunclassified

“…В попередніх дослідженнях (Rublenko, et al, 2014;Rublenko, et al, 2015) було показано, що використання кальціє-фосфатної кераміки з різними частками αта/чи β-трикальційфосфатів забезпечує надійний остеокондуктивний ефект, завдяки чому гістоморфологічно скорочується запально-резорбтивна фаза репаративного остеогенезу, стадія ретикулофібринозного і хрящового регенератів, більш ранніми виявляються процеси ремоделювання кісткового регенерату. В дослідженнях на собаках це верифікується низкою маркерів кісткового метаболізму -кісткової лужної фосфатази, тартрат -резистентної кислої фосфатази, остеокальцина і с-телопептидів колагену І типу.…”

Section: обговоренняunclassified

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Dynamics of cytokines in osteosis splinter fractures replacement in dogs with hydroxyapatite ceramics silicon-doped

et al. 2021

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Among the entire public of fractures of long bones, more complications of reparative osteogenesis occur fragmental fractures. Their significant defects, which arise as a result of the removal of non-viable fragments, cause disturbances in local hemodynamics and microcirculation and loss of osteoconduction in the area of injury, lead to a loss of the reparative potential of bone tissue in the defect zone, and the methods of osteosynthesis do not provide restoration of osteoconduction and do not have osteointegration properties. For osteosis, a number of composite materials have been proposed, among which ceramics based on calcium phosphates are considered the most promising, which is confirmed by previous clinical, radiological and histomorphological studies. The aim of the study is to determine the dynamics of the blood levels of TGF-β and interleukins IL-1β and IL-10 for osteosis replacement of splinter bone fractures in dogs with calcium phosphate ceramics doped with silicon. Material and research methods: a control (n = 7) and research group (n = 7) dogs with accidental splinter fractures of long bones were formed. In both groups, extracortical osteosynthesis was performed with a support plate from an unalloyed titanium alloy. In the control group, bone defects were left to heal under a blood clot, and in the experimental group they were replaced with ceramics (GTlKg-3). Blood samples were taken after trauma no later than on the 48th day, and on the third, twelfth, twenty-first, forty-second and 60th days after surgical treatment. The enzyme immunoassay was used to determine the serum levels of interleukins IL-1β and IL-10 and transforming growth factor (TGF-β) тhat for a bureta reaction is a bit of a home-made bottle. The study results showed an anti-inflammatory cytokine profile with physiological norms in dogs. In case of bone injury, it becomes pro-inflammatory in nature, triggers the process of osteoresorption in the fracture area. At the same time, during reparative osteogenesis after extracortical osteosynthesis for splinter fractures, proinflammatory cytokinemia acquires a permanent character with a peak on the forty-second day, and this implies inhibition of metaplasia of cartilaginous regenerate into bone, that is, it slows down the consolidation of the fracture. However, in the case of osteosis with ceramics, the level of pro-inflammatory cytokinemia is significantly lower and continues during the first 12 days with a peak on the third day, during the inflammatory-resorptive stage. However, the key in understanding the effectiveness of osteosis was the dynamics of TGF-β, which showed its biphasic hyperreactivity in control animals, while in experimental animals, its balanced production was noted. Conclusion. Osteosis replacement of bone defects in long tubular bones in dogs with hydroxyapatite with β-tricalcium phosphate ceramics doped with silicon is accompanied by moderate cytokinemia in accordance with the course of the inflammatory-resorptive stage and the phase of remodeling with a peak of induction of transforming growth factor during the period of active osteogenesis forty-second course of reparative osteogenesis.

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“…These requirements are mostly met by the materials based on calcium-phosphate ceramics, which for their biological properties are called bioactive ceramics (Huryn et al, 2012;Oliveira et al, 2017;Deveci et al, 2020). Earlier, we (Rublenko et al, 2018;Chemerovskyi, 2020;Rublenko et al, 2020) conducted X-ray, macromorphological and hematological evaluations of osteointegrational properties of samples of calcium-phosphate ceramics of hydroxyapatite and β-tricalcium phosphate and alloyed with Si with various physical-chemical properties, which were used on rabbits with modeled fractures and dogs with complex diaphyseal bone fractures of long bones, which were brought to the University Clinic of Small Pets.…”

Section: Introductionmentioning

confidence: 99%

Effect of implants of hydroxyapatite with tricalcium phosphates alloyed with Si on histomorphological and biochemical parameters in cases of bone defects of rabbits

Chemerovskіy

Rublenko

et al. 2021

Regul. Mech. Biosyst.

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Complex comminuted fractures are accompanied by development of bone defects and loss of reparative potential of the bone tissue in the region of the trauma. This brings the necessity of using implants with optimum osteoconductive and osteointegration properties. The objective of the study was determining the condition of biochemical bone markers and peculiarities of histomorphological changes under the influence of ceramic hydroxyapatite (HA) implants with various physical-chemical properties in the conditions of diaphyseal bone defects in rabbits. We composed control and experimental groups of rabbits with 10 individuals in each with diaphyseal bone defects (3 mm) of the radial bones formed under general anesthesia. In one experimental group, they were filled with granules of hydroxyapatite with α-tricalcium phosphate, and in the second group – with β-tricalcium phosphate, alloyed with Si. In the control rabbits, the defects healed under a blood clot. Blood was analyzed on the 3rd, 7th, 14th, 21st and 42nd days, and as reference we used biochemical parameters of blood of clinically healthy rabbits (n = 10). Bone biopsied materials were taken on days 21–42 under general anesthesia. When using hydroxyapatite with β-tricalcium phosphate, alloyed with Si, we determined early intensification of the levels of nitrogen oxide, angiogenesis and development of bone regenerate in conditions of shortening of inflammatory resorption phase, which was verified according to the level of tartrate-resistant acid phosphatase. According to the level of bone isoenzyme of alkaline phosphatase in the blood serum of animals of the control group, the reparative osteogenesis developed slowly and peaked on day 42, whereas in animals implanted with α-tricalcium phosphate, its development peaked peaked on days 14–42, and when using Si-alloy – on days 7–14. Histomorphologically, on the 21st day, in the case of replacement of bone defect with hydroxyapatite with α-tricalcium phosphate, coarse-fibered type of bone regenerate developed with no dense contact with the elements of the regenerate, while spongy bone trabeculae occurred when hydroxyapatite was applied with β-tricalcium phosphate alloyed with Si, and the control rabbits were observed to be in the stage of cartilaginous callus. On the 42nd day, under the influence of implants of hydroxyapatite with α-tricalcium phosphate, the spongy bone tissue transformed into compact tissue with further mineralization. With implants alloyed with Si, there occurred compact bone tissue, and bone regenerates of the control animals were regions of coarse-fibered and spongy bone tissue without dense contact with the parent bone. This study revealed that hydroxyapatite with β-tricalcium phosphate alloyed with Si had notable osteoinductive and osteointegrating properties, as indicated by early angiogenesis and osteoblast reaction, positive dynamics of the marker biochemical parameters with faster and better development of bone regenerate as spongy bone trabeculae.

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Evaluation of usage of silicon-doped hydroxyapatite ceramics for treatment of fragmented bone fractures in dogs

Cited by 5 publications

References 11 publications

Clinical-radiological, hematological and biochemical assessment of bone replacement with germanium-doped calcium-phosphate ceramics for fragment fractures of tubular bones in dogs

Clinical-radiological, hematological and biochemical assessment of bone replacement with germanium-doped calcium-phosphate ceramics for fragment fractures of tubular bones in dogs

Dynamics of cytokines in osteosis splinter fractures replacement in dogs with hydroxyapatite ceramics silicon-doped

Effect of implants of hydroxyapatite with tricalcium phosphates alloyed with Si on histomorphological and biochemical parameters in cases of bone defects of rabbits

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